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Gracilis were applied to investigate the effect of proplastid on adaptability, in which such structure was found to be important to the competitiveness during heterotrophic growth in the dark; 2 a new procedure for differential display technology was developed and used to study the influence of plastid on nuclear gene expression, with which it was shown that the loss of plastid could cause remarkable changes in expression of certain nuclear genes.
本文主要有两点突出之处:1)应用裸藻褪色突变株与野生型的生长竞争实验探讨了原质体对于裸藻的适应意义,发现在黑暗环境和异养生长条件下原质体对于细胞的生存竞争有重要作用;2)改进了一种差异显示技术并利用该技术来研究质体的有无对基因表达的影响,证明质体丢失可导致某些核基因表达的显著变化。
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Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.
将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇/卵磷脂/十八胺脂质体包裹,免疫2周龄SPF鸡,4周后用同型禽流感病毒进行人工感染,1周后采集泄殖腔分泌物分离病毒,结果未免疫组6/6分离到病毒,裸质粒DNA免疫组1/6分离到病毒胆固醇/卵磷脂/十八胺脂质体包裹DNA免疫组1/6分离到病毒,DC-chol脂质体DNA免疫组没有分离到病毒(0/6):人工感染后1周各组的HI抗体(Log2)分别为6.38±0.98,7.00±1.26,7.83±1.17,8.00±0.89,2周后为8.50±0.55,8.67±0.82,8.68±0.45,9.33±0.52,脂质体包裹组在同期均高于未免疫组和裸DNA免疫组,表明脂质体包裹质粒DNA免疫动物后,能增加动物对病毒感染的抵抗力和反应能力。
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Perhaps it is due to its role in adaptation that A. longa and other bleached species of euglenoids retained their proplastid structure.
或许正由于原质体结构在竞争适应中的效应,长变胞藻和自然界某些裸藻褪色种仍保留有这一结构。
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gymnosophist:秘密信仰的信奉者/修行者/裸体主义者
gymnoplast /裸质体/ | gymnosophist /秘密信仰的信奉者/修行者/裸体主义者/ | gymnosperm /裸子植物/
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gymnosophist:秘密信仰的信奉者
gymnoplast 裸质体 | gymnosophist 秘密信仰的信奉者 | gymnosophistnudist 裸体主义者
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gynandromorph:雌雄嵌合体
gymnoplast|裸质体 | gynandromorph|雌雄嵌合体 | gynandromorphism|雌雄嵌合体
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periplast:周质体
裸藻细胞的最外层是原生质膜,在质膜内,由蛋白质构成周质体(periplast),因此,周质体是细胞的内部构造. 电子显微镜下观察,周质体是由多条壁纹(stripe)密接组成的,这些壁纹螺旋状包围着藻体. 有些种的周质体薄,易弯曲,藻体能变形.
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periplast:[生]质膜,(尤指裸藻的)周质体
crescent screen 月牙筛, 扇形筛 | periplast [生]质膜,(尤指裸藻的)周质体 | trumpetcall 集合号声 紧急召唤
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Euglenales:裸藻目
本纲分两个目,即裸藻目 (Euglenales)和柄裸藻目 (胶柄藻目)(Colaciales). 二、裸藻门的代表植物 (一)裸藻属(Euglena) 属于裸藻目. 细胞纺锤形、长纺锤形或圆柱形,前端宽而钝圆,后端锐, 无甲鞘. 周质体的弹性大小,因种而异. 有两根鞭毛,
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gymnoplast:裸质体
guttation|吐水 | gymnoplast|裸质体 | gynandromorph|雌雄嵌合体