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The evolutionary relationships were investgated by comparing the results from molecular data alone or molecular data combined morphological characters using different analysis methods: maximum parsimony by PAUP(superscript *) 4.0 and Bayesian inference by MrBayes 3.0B4. Base composition and ti/tv of 28S rDNA D2 regions were analyzed by PAUP(superscript *) 4.0. The results showed that GC content of the 28S rDNA D2 gene sequences of subfamily Euphorinae ranges from 40.00% to 49.25%. Transversion frequency of sequence variable sites among species of Euphorinae was higher than that of transition. The subfamily Euphorinae was proved to be a paraphyletic group with close relationship to subfamilies Neoneurinae and Cenocoelinae; only two tribes, Meterorini and Microctonini, were proved to be monophyletic groups; Centistini, Cosmophorini, Euphorini and Dinocampini were paraphyletic groups; the basal position of tribe Meterorini within Euphorinae was partially supported by our phylogenetic trees, and tribe Microctonini was proved to be a group at the top of the trees.
结果表明：优茧蜂亚科的28S rDNA D2基因序列片段的GC%含量在40.00%~49.25%之间变动，而对于碱基替代情况来讲，优茧蜂亚科各个成员间序列变异位点上颠换大于转换；不同的分析和算法所产生的系统发育树都表明目前根据形态定义出的优茧蜂亚科Euphorinae不是一个单系群，而是一个与蚁茧蜂亚科Neoneurinae和高腹茧蜂亚科Cenocoelinae混杂在一起的并系群；在优茧蜂亚科内部，悬茧蜂族Meterorini和食甲茧蜂族Microctonini（排除猎户茧蜂属Orionis）为单系群，而宽鞘茧蜂族Centistini、大颚茧蜂族Cosmophorini、优茧蜂族Euphorini、瓢虫茧蜂族Dinocampini为并系群；悬茧蜂族Meterorini在优茧蜂亚科Euphorinae内位于基部位置的观点得到部分的支持，同时食甲茧蜂族Microctonini被判定为相对进化的类群。
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Chrysididae：(七)青蜂科
黑蚁 Lasius niger Linnaeus | (七)青蜂科 Chrysididae | 上海青蜂 Chrysis sbanghaiensis Smith
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Chrysis shanghaiensis Smith：上海青蜂
多寄生于单栖性蜂类,如上海青蜂(Chrysis shanghaiensis Smith)寄生于刺蛾的幼虫. 蜾蠃科采蜜生活,青蜂科,葉蜂科昆虫,叶蜂科昆虫,青蜂科昆虫生活,青蜂科昆虫,膜翅目昆虫科,膜翅目蜂科昆虫,胡蜂科昆虫生活,蜾蠃科昆虫生活,蚁科昆虫
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Eucharitidae：蚁小蜂科
Eucharist /圣餐/ | Eucharitidae /蚁小蜂科/ | Euchorda /真脊索动物亚门/
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Ichneumonidae：茧蜂科
蚁科Formicidae 665 45118 + + + 688 48101 + + + 4115 93142 + + + | 茧蜂科Ichneumonidae 8 0154 + 11 0177 + 16 0136 + | 细蜂科Proctotrupidae 4 0127 +
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Mutilla：蚁蜂属
Mutilacea 贻贝亚目 | Mutilla 蚁蜂属 | Mutillidae 蚁蜂科
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Mutillidae：蚁蜂科
有4个重要的独栖黄蜂类群为寄生性,并不营巢,这些类群是肿腿蜂超科青蜂科(Chrysididae)的杜鹃黄蜂和土蜂超科臀沟土蜂科(Tiphiidae)的臀沟土蜂、土蜂科(Scoliidae)的土蜂及蚁蜂科(Mutillidae)的蚁蜂.
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Mutillidae：蚁蜂科;土蜂科
裸兽组 MUTICA | 蚁蜂科;土蜂科 MUTILLIDAE | 突变元;突变单位 muton
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Pselaphidae：蚁冢虫科
鳖甲蜂科;玳瑁蜂科 PSAMOCHARIDAE | 蚁冢虫科 PSELAPHIDAE | 触角鞘;须鞘 pselaphogheca
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Scoliidae：土蜂科
北半球温带地区最为人熟知的社会性黄蜂是长脚黄蜂属(Polistes)、大胡蜂属(Vespa)和小胡蜂属(Vespula)的种有4个重要的独栖黄蜂类群为寄生性,并不营巢,这些类群是肿腿蜂超科青蜂科(Chrysididae)的杜鹃黄蜂和土蜂超科臀沟土蜂科(Tiphiidae)的臀沟土蜂、土蜂科(Scoliidae)的土蜂及蚁蜂科(M
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Xylocopidae：木蜂科
是昆虫纲中较大的目,但危害木材的只有三个科,即蚁科(Formicidae)、大木蜂科(Xylocopidae)和树蜂科(Siricidae). 主要有船蛆(Teredo)、节铠船蛆(Bankia)、和马特海笋(Martesia)等三属的一些海生生物蛀蚀在海水中的木材.