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Based on a new skeleton of pyrrol coumarin, we synthesized 21 derivatives with electrowithdrawing and electrodonating groups at several sites on two classes of core structures.
同时我们还设计了一类新型的高效荧光素,它的基本骨架是由吡咯并香豆素构成,是现代FRET技术的理想供体荧光团。
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Thin leaf section were prepared from fresh tissues of YS-lnfected and healthy citrus plants, when observed under a fluorescence microscope (Type, Olympus AH-FL-2), a yellow fluorescence was distinctly observed in the YS-infected phloem tissues and not in the phloem tissuses of healthy plants and of those infected with tristeza, exocortis, canker, scab, greasy...
将健叶和黄龙病叶叶柄用保险刀片徒手切片,在透射式荧光显微镜下检查,可看到健叶叶柄的木质部导管细胞壁发黄色荧光,韧皮纤维细胞发绿色荧光,而病叶叶柄韧皮部中有1~多个鲜明的黄色或黄绿色荧光团块。这种特异性的荧光,在健叶柄韧皮部中是没有的,其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦没有此种特异性荧光。
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The major contents in this thesis are described as follows:(1) Seven new thiol fluorescence reagents containing 2-phenylbenzoxazole (or 2-phenylbenzothiazole) derivatives or benzanthrone as fluorphore and iodinacetylamine or maleimide as reaction groups have been synthesized.
本论文的研究工作主要分为以下几个方面:(1)以碘乙酰胺或马来酰亚胺为反应基团和以2-芳基苯并噁唑类化合物或苯并蒽酮为荧光团,首次合成了七种新的碘乙酰胺类和马来酰亚胺类巯基荧光试剂。
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fluorogen:荧光团
fluorofibre 含氟纤维 | fluorogen 荧光团 | fluorogenicsubstrate 荧光底物