- 更多网络例句与荧光相关的网络例句 [注：此内容来源于网络，仅供参考]
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It is found that the fluorescence intensity is increased with the elevation of temperature when the quencher diphenylamine is added to PU-DFY, which is an inverse phenomenon against the general fluorescent materials.
研究还发现，加入二苯胺猝灭剂的PU-DFY，荧光强度随温度的升高而增强，与一般荧光物质荧光强度的温度特性相反。关键词：聚氨酯-分散荧光黄；高分子荧光染料；水分散体；荧光
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Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.
该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。
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The two Fraunhofer lines at 688nm and 760nm are obvious in the radiance spectra by ASD FieldSpec Pro NIR spectrometer,which largely overlap the chlorophyll fluorescence emission spectrum of leaves.
第三，研究并分析了夫琅和费暗线方法计算的688nm和760nm波段的荧光特性，结果表明该方法计算的荧光是可靠的，它与光合有效辐射关系密切，复相关系数达到了0.9；冬小麦冠层荧光光谱在760nm和688nm波段的荧光大小基本相等，而地锦冠层荧光光谱在688nm波段的荧光强度是760nm的3倍左右，表明荧光光谱能够更加敏感地反映植被物种或生理生化状况的差别。
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Firefly can give rise to luminescence during the night, the basic reaction involved the firefly luciferase and luciferin and ATP in the body of firefly.
萤火虫在夜晚可以发出荧光，其基本的生物化学反应是虫荧光素酶可以催化荧光素生成氧化荧光素并且放出荧光，可以用以下这个反应方程表示：荧光素+ATP+O_2？
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Results (1) CCD fluorescent microscope was superior to laser scanning confocal microscope in collecting the fluorescent image of DCF. CCD fluorescent microscope was chosen to be the system applied in this research with the consideration of operation, cost and et al. The mercury-arc lamp of fluorescent microscope was fit for the requirement of our study. The wavelength range of the light output was 460-490 nm and the power density was about 100mW/cm in the condition of our study. Organelle-cell fluorescence intensity ratio analysis was more suitable than other methods.
结果：(1)CCD荧光显微镜采集到的DCF荧光图像的质量优于共聚焦显微镜，同时综合考虑包括采图质量、光照射剂量的可控性、实验成本和操作简便性等诸多因素，最终确定了CCD荧光显微镜作为实验研究的工具；荧光军医进修学院硕士学位论文中文摘要显微镜的汞灯输出功率稳定，光斑均匀，经本实验所选取的光路系统后输出波长范围为460一490nm，功率密度约为1 00 mw/cm；细胞器一细胞荧光强度比值法对于荧光分布位置的确定优于其他方法。
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In this paper, we studied the relationshipbetween the concentration, acidity, temperature, heavy metalic ions and the fluorescencequenching of fluorescein. The result showed that when the concentration was over 1. 55 ×10-5 mol/L, the fluorescence quenching strengthened with the increase of f...
结果表明：荧光素浓度大于1.55×10－5mol·L－1时，随着浓度增大，荧光猝灭作用增强；酸度对荧光猝灭作用有明显作用，PH值小于8.5时，随着PH值升高而减弱，PH值大于8.5时，随着PH值升高而增强；温度对荧光猝灭作用呈线性关系，温度升高，荧光猝灭作用减弱；重金属离子对荧光猝灭有显著的作用，是影响眼底血管造影质量的重要因素。
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The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.
结果表明，胭脂红在波长330～430 nm的光激发下，产生较强荧光，荧光峰值波长为621 nm，最佳激发波长为376 nm；苋菜红在波长300～440 nm的光激发下，产生较强荧光，荧光峰值波长为643 nm，最佳激发波长为370 nm；柠檬黄在波长280～380 nm的光激发下，产生很强荧光，荧光峰值波长为565 nm，最佳激发波长为315 nm；日落黄在波长310～410 nm的光激发下，产生较强荧光，荧光峰值波长为592 nm，最佳激发波长为348 nm；亮蓝在波长320～390 nm的光激发下，产生较强荧光，荧光峰值波长为456 nm，最佳激发波长为350 nm。
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The results showed that: three major peaks,humuslike fluorescence peak A,proteinlike fluorescence peaks B and C appeared on the threedimensional fluorescence spectroscopy;the DOM fluorescence intensity was well related with DOC and CODMn,indicating that fluorescence dissolved organic matter content can reflect both the organic carbon content and the organic matter content by oxidation in the water in August,during the nonalgae bloom outbreak period,fluorescence intensity had a poor correlation with chlorophyll a,indicating that phytoplankton was not the main source of the fluorescent substances.
结果显示：小江回水区DOM的三维荧光光谱主要表现出3个峰，类腐殖质荧光峰A、类蛋白荧光峰B和C；DOM荧光强度与DOC、CODMn相关性较好，表明荧光溶解有机物质的含量可以较好反映水体中有机碳和可被氧化有机物的含量；在8月份非水华暴发时期荧光强度和叶绿素a相关性较差，表明DOM荧光物质主要不是由浮游植物产生。
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Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.
本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。
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The intensity ratio of TO and LO inMCT was observed to be different. Such difference was explained in terms of the different Ramangeometry arrangement.〓. The laser-induced micro-photoluminescence in the range of 1000～5000〓(1.34eV～1.83eV) was found for the first time in LPE MCT epilayer. The center of photoluminescence wasat 2750〓 or 1.62eV and the FWHM of luminescence was 2000〓 or 0.25eV. We assume thatthe photoluminescence is due to recombination of electron from an anion vacancy resonance levelto the top of valance. In addition, new Raman shift was observed at 750〓 in LPE MCTepitaxial film.〓. The laser-induced micro-photoluminescence with quasi-periodic structure was observed forthe first time at room temperature in one of MOVPE MCT epitaxial film samples. The range offluorescence was from 1.46eV to 2.21eV, i.e., 1.73eV above the conduction band edge.
2首次在LPE生长的碲镉汞外延薄膜的显微Raman谱中，在1000～5000〓范围发现了激光激发显微荧光，该荧光的发光范围换算为电子伏特标度为1.34eV～1.83eV，荧光的发光中心大约位于2750〓，即1.62eV，发光的半峰高宽约为2000〓或0.25eV；指出该显微荧光来源于碲镉汞薄膜中的阴性离子空位共振能级的激光激发发光；观察到了碲镉汞外延薄膜中一个新的Raman散射峰，位于750〓位置； 3首次在一块用MOVPE方法生长的〓Te外延薄膜的显微Raman谱中，发现了1.46eV至2.21eV范围并伴随有周期结构的显微荧光峰，该发光峰对应的能带中心位于〓Te材料导带底上方1.73eV，通过研究得出样品在1.46eV至2.21eV范围的显微荧光峰是由于改进 MOCVD 生长工艺，提高了碲镉汞外延薄膜的结构质量所致；通过分析指出该显微荧光来源于外延层中的阴性离子空位的共振能级发光。
- 更多网络解释与荧光相关的网络解释 [注：此内容来源于网络，仅供参考]
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bioluminescence：生物荧光
荧光素酶可以催化luciferin氧化成oxyluciferin,在luciferin氧化的过程中,会发出生物荧光(bioluminescence). 然后可以通过荧光测定仪也称化学发光仪(luminometer)或液闪测定仪测定luciferin氧化过程中释放的生物荧光.
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fluorescein：荧光素,荧光黄
fluorenylmethyloxycarbonyl|芴甲氧羰基[可用作氨基保护剂] | fluorescein|荧光素,荧光黄 | fluorescein isothiocyanate|异硫氰酸荧光素
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fluorescein isothiocyanate：异硫氰酸荧光素
fluorescein|荧光素,荧光黄 | fluorescein isothiocyanate|异硫氰酸荧光素 | fluorescence body|荧光小体
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fluorescein test：荧光素试验
fluorescein paper 荧光素试纸 | fluorescein test 荧光素试验 | fluorescein 荧光素;荧光黄
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fluorescence microscope：荧光显微镜
1.荧光显微镜 荧光显微镜(fluorescence microscope)是用来观察标本中的自发荧光物质或以荧光素染色或标记的细胞和结构. 荧光显微镜是以高压汞灯产生的短波紫外线为光源,并配有激发、阻断、吸热和吸收紫外线等滤片系统,标本中的荧光物质在紫外线激发下产生各种颜色的荧光,
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fluorescent screen：荧光板,荧光屏
fluorescent radiation 荧光放射,荧光辐射 | fluorescent screen 荧光板,荧光屏 | fluorescent staining 荧光染色法
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fluorimeter：荧光光度计/荧光计
fluoride /氟化物/ | fluorimeter /荧光光度计/荧光计/ | fluorimetry /荧光测定法/
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fluorometer：荧光光度计
根据光学系统结构的不同,荧光测定装置可分为荧光光度计(fluorometer)、荧光分光光度计(fluorospectrophotometer)分光荧光光度计(Spectrofluorophtometer)三大类.
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luciferase：荧光素酶
组织特异性基因表达: 荧光素酶(luciferase)是一类生物发光酶, 其中的renilla荧光素酶和firefly荧光素酶分别识别不同的底物, 一种细胞可被这两种荧光素酶标记: renilla 荧光素酶基因由一组成性稳定表达的启动子驱动, 作为内参,
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fluorescin：荧光生/氢化荧光素
fluorescer /荧光剂/荧光增白剂/ | fluorescin /荧光生/氢化荧光素/ | fluorescite /荧光素钠/