- 更多网络例句与色析法的相关的网络例句 [注:此内容来源于网络,仅供参考]
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Methods The protein -binding rate of Qingxin tong was estimated by protein equili-bration dialyzator and was determin ed by HPLC.
方法应用平衡透析法了解青心酮在早、晚孕妇血清中的蛋白结合率,并用HPLC色谱法进行定量检测,对结合率及相关参数进行比较。
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Methods: Take β-sitosterol,succinic acid and guanosine as targets,quality criteria was studied on collected Rhizome Pinelliae Preparata by TLC and HPLC.
以β-谷甾醇、琥珀酸和鸟苷为指标,采用薄层层析法和高效液相色谱法对收集的姜半夏进行质量标准研究。
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Wβe measured the levels of MDA in plasma and ultrafiltrate, Scr and 2-MG of 23 ESRD patients undergoing regular maintained on HD before and after CAVH compared with 23 healthy people as control by using TAB chromatometry, radioimmunoassay and base saturation picric acid test.
MDA方法:应用硫代巴比妥酸比色法、放射免疫法、饱和苦味酸法分别检测例维持性血液透析23ESRDHD的患者治疗前后血浆和超滤液中、血清βCAVHMDA2微球蛋白(β-2)和血清肌酐水平,并与例健康人对照。
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The results showed that the variation of the color display were not significantly corelated with the composition of the photopigments of their zooxanthella.
研究方法主要是藉色层分析法,如滤纸色层分析法,薄层色层分析法及高压液相层析法等方法以达分离、分析和定量色素的目的。
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These three kinds of Bile extraction were qualitatively detected by using the paper chromatography and infrared spectral scanning method, and the results show that These extractions have the same Rf value and infrared spectra's characteristic absorption peak as choleic acid, glycocholic acid and taurocholic acid standard preparation which can prove that These three kinds of Bile extraction are choleic acid, glycocholic acid and taurocholic acid respectively.
采用薄层层析法和红外光谱扫描法对提取物进行定性检测,结果证明三种提取样品分别与胆酸、甘氨胆酸及牛磺胆酸标准品显色斑点Rf值相同,红外光谱特征吸收峰均一致,表明提取出的三种样品分别为胆酸、甘氨胆酸、牛磺胆酸结晶体。
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Methods Five different ultraviolet absorption components were isolated from the filtrate of Botrytis cinerea isolate 7–3 culture. Of the five components, one showed strong inhibitory to Digitaria sanguinalis.
在前期薄层层析法、柱层析法和高效液相色谱法分析的基础上对灰葡萄孢诱变菌株BC7–3的代谢产物中具有除草活性的5个不同组分分别进行了液相色谱制备。
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The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.
实验部分采用以石油醚脱脂,甲醇为溶剂的回流提取法来提取植物多酚,用显色反应检识酚羟基;采用紫外分光光度法对多酚粗体物进行总多酚含量的测定;选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌,采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验,并测定其最低抑菌浓度;采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析,根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。
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It is a organic hexahydric weak acid. In different acidity, the dissociation degree and the color of reagent solvent were different also. Determined and draw the absorption curve of reagent in varies acidity, and figured out the dissociation constant by Perisic-Janjic method; the complex ratio of the reagent and bismuth was determinated by mole ratio method and continuous variation method; The apparent stability constant of the complex was calculated, the mechanism of dissociation and coordination were discussed.
2,6—二溴4—硝基偶氮氟胂是本实验室合成的测定铋的一个较好的试剂,对其进行了进一步的研究,在用柱层析法对其进行了提纯后,测定了试剂在不同酸华东师范大学硕:l:学位论文摘要度下的吸光度和最大吸收波长,绘制了其吸收曲线,用Perisic一Janjic法计算了试剂的六级离解常数;用摩尔比法和连续变化法测定了试剂与秘形成的配合物的络合比:计算了配合物的表观稳定常数,研究了其离解和配合机理,为筛选高效有机显色剂提供了理论依据。
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Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.
针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。
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Quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E wereisolated from the flower of Acanthopanax senticosus. Searching bioactive natural compound and establishing quality evaluation standard arefocused on this experiment. Using cold immersing of 70% methanol to extract from flower ofAcanthopanax senticosus and integrating macroporous resin, silica gel column chromatography,sephadex gel to abstract. We obtained five monomer compounds (compoundⅠ-compoundⅤ),quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E. That five compoundswere determined by HPLC in eight parts of Acanthopanax senticosus (flower, root, leaf fruit,shoot, new shoot, phloem of shoot, skin of shoot), which provide science bases for establishingquality evaluation system and settle infarctate foundation.
本文以从刺五加花蕾中寻找具有重要生物活性的天然化合物和建立质量评价标准为主要目的,对其化学成分进行研究,采用70%甲醇冷浸的方法对刺五加花蕾中的成分进行提取,运用大孔树脂吸附色谱法、硅胶柱层析法以及葡聚糖凝胶柱层析相结合的方法进行分离,得到5种单体化合物,分别为槲皮素、槲皮素鼠李糖苷、金丝桃苷、紫丁香苷和刺五加苷E;利用高效液相色谱法对这5种化合物在刺五加花蕾中的含量进行了测定;考察了8个部位(花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮)中这5种化合物的含量分布情况,并作一比较,为今后建立刺五加质量评价体系提供了科学依据,奠定了坚实的基础。
- 更多网络解释与色析法的相关的网络解释 [注:此内容来源于网络,仅供参考]
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adsorption chromatography:吸附层析
他用此法从而证明了绿叶中不仅有叶绿素,还存有其他色素,并将此法定名为色层分析法,继后他研究了百余种吸附剂,奠定了吸附层析(adsorption chromatography)的一些基础.
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affinity chromatography:亲和层析法
(四)根据配体特异性的分离方法-亲和色谱法 亲和层析法(affinity chromatography)是分离蛋白质的一种极为有效的方法,它经常只需经过一步处理即可使某种待提纯的蛋白质从很复杂的蛋白质混合物中分离出来,而且纯度很高.
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chromatography:层析法
[目录] 基本知识 特点 基本原理 层析分类 [原文] 基本知识 层析法(Chromatography)又称色谱法、色层法或层离法,是目前广泛应用的分离技术. 层析法是利用不同物质的某些理化性质的差异而建立起来的技术. 组成 固定相
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paper chromatography:纸色谱
纸色谱(paper chromatography)又称为纸层析,是在滤纸上进行的色谱分析法. 滤纸被看作是一种惰性载体,滤纸纤维素中吸附着的水分或其他溶剂,在层析过程中不流动,是固定相;在层析过程中沿着滤纸流动的溶剂或混合溶剂是流动相,又称展开剂.
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displacer:置换剂
是指样品输入色谱柱后,用一种与固定相作用力极强的置换剂(displacer)通人色谱柱,去替代结合在固定相表面的溶质分子. 样品在置换剂的推动下沿色谱柱前进,使样品中各组分按作用力强弱的次序. ③亲和层析法(aflinity chromatography)是分离蛋白质的一种极为有效的方法.
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GC:气相色谱法
方法: 根据文献分类综述,内容包括样品处理和分析方法如高效液相色谱法(HPLC)、气 相色谱法(GC)、气相色谱-质谱联用法(GC-MS)、放射免疫分析法(RIA)等,并对微 量透析法被引入经皮给药动力给药系统研究的体内分析方法进行综述.
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Achatina fulica:非洲大蜗牛
利用硅胶柱层析法和薄层层析法(TLE)分离乙酸乙脂浸泡非洲大蜗牛(Achatina fulica)得到的粗提物,对各纯化物的抗菌活性进行测定,并用气相色谱-质谱法技术分析抗菌物质的主要化学成分.结果表明其头足部和内脏团的主要抗菌物质成分不同,
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chromatographic:色析法的
chromatographer 色析法 | chromatographic 色析法的 | chromatographically 色析法地
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chromatographic:色析法的, 层离法的
chromatographic technique | 色谱技术 | chromatographic | 色析法的, 层离法的 | chromatographically | 色析法地, 层离法地
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Cholesterol from Human Gallstones:(从人的胆结石中提取胆固醇)
3. Melting and Boiling Points (熔点,沸点的测定). | 4. Cholesterol from Human Gallstones (从人的胆结石中提取胆固醇). | 5. Thin-layer Chromatograph (薄层析色谱法).