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Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.
体外培养SH-SY5Y人脑神经母细胞瘤细胞,在培养液中加入不同浓度的氟化物或加入抗氧化剂,培养48h后用测定细胞MTT的方法来了解细胞的损伤程度,用高效液相色谱法分离和测定培养液中脂质过氧化物水平,用过柱和比色法测定细胞生物膜磷脂含量,用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。
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Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.
动脉瘤管壁厚薄明显不均,全层或局部区域显著变薄向外膨出,内皮细胞空泡变性或坏死脱落,部分内皮细胞剥离并突入管腔成游离状,可见血栓形成及部分血栓机化;内弹力板变薄、消失或突然中断;在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原;部分动脉瘤壁呈均质状玻璃样变,平滑肌细胞明显减少;中膜和外膜可见大量的炎性细胞浸润;瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞,胞浆内充满脂类物质及胆固醇结晶;部分动脉瘤腔内充满血栓,有的血拴已经纤维化,血栓基部机化,血栓表面有红细胞和纤维素。
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lipoblastoma:脂母细胞瘤
lipoblast 成脂细胞 | lipoblastoma 脂母细胞瘤 | lipoblastomaliposarcoma 脂肪肉瘤
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lipoblastoma:脂肪肉瘤 脂母细胞瘤
lipoblast 成脂细胞 | lipoblastoma 脂肪肉瘤 脂母细胞瘤 | lipoblastomatosis 脂母细胞增生症