- 更多网络例句与胰肽酶相关的网络例句 [注:此内容来源于网络,仅供参考]
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However, commercially available CPB purified from porcine pancreas was very expensive, and was not totally free of other proteases, such as trypsin and chymotrypsin.
到目前为止,商业途径获得的羧肽酶B 主要从猪胰脏中提取,使得产品价格昂贵,同时也不可避免地混杂有其它蛋白酶,如胰蛋白酶,胰凝乳蛋白酶。
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In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.
第一部分,我们按照交替氧化酶(Alternative Oxidase,AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列,用固相法合成由12个氨基酸残基组成的多肽,将此多肽与α-牛胰凝乳蛋白酶原A相连,用作半抗原,免疫家兔制备人工合成12肽的抗体。
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The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.
根据氨基酸自动分析仪测定的氨基酸组成和精确分子质量计算出NSPC分子总共有331个氨基酸残基,分别是由40个Asp、22个Thr、18个Ser、22个Glu、1个Gly、31个Ala、27个Cys、17个Val、13个Ile、22个Leu、5个Tyr、7个Phe、7个Lys、24个His、51个Arg、14个Pro、1个Met和9个Trp组成。胰蛋白水解酶分别酶解NSPC-1和NSPC-2后,分别采用MALDI-TOF-MS分析,其肽指纹谱图显示胰蛋白酶可将NSPC-1和NSPC-2水解成大小不等的多条肽段,质量数在800以上的NSPC-2有11段,即水解的肽质量分别为899.54、1454.77、1544.9、1683.87、1803.93、1811.91、1987.92、2003.90、2536.26、2915.42和3492.72。
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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2~0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%~80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0~0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.
本研究考察了各种纯化方法,将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合,建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺:胰酶用pH4.5醋酸缓冲溶液提取后,粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5,洗脱缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5)含0.2~0.35mol/LNaCl分两步洗脱,收集激肽释放酶的洗脱峰;DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液:0.01mol/LTris-HCl缓冲液(pH7.5,洗脱液:0.5mol/LNaCl溶液,一次洗脱,收集胰蛋白酶洗脱峰;最后,亲和层析未吸附液用30%~80%硫酸铵分级盐析处理,沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5含1mol/L(NH_4)_2SO_4,洗脱缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5)含0~0.6mol/L(NH_4)_2SO_4,分两步洗脱,收集糜蛋白酶的洗脱峰。
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PACAP is a pleiotropic neuropeptide that belongs to the secretin/glucagon/vasoactive intestinal peptide family.
垂体腺苷酸环化酶激活肽是多效性的神经肽,属于肠促胰液素/胰高血糖素/肠血管活性肽家族。
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We make chloroacetylaion to N-terminal of Asp, and then convert this compound to GD dipeptide by aminolysis. Followed the lipase -catalyzed peptide synthesis of RGD in aqueous water-miscible organic cosolvent is studied. We set up a new efficient reaction method to synthesize RGD tripeptide. The problem that peptide containing polar amino acid can not be synthesized readily in organic solvent was solved. We offer a new experimental proof for PPL-catalyzed peptide synthesis and make a more general analysis for the effect of some factors in theory.
采用氯乙酰基保护天冬氨酸,然后进行氨解生成GD二肽,利用猪胰脂肪酶催化合成RGD三肽,着重解决了亲水氨基酸/肽片段底物的溶解性与合成产率低的问题,建立了一种新的有效合成RGD三肽的反应模型,并探索解决了极性较强的氨基酸的酶促肽合成的问题,对脂肪酶催化合成肽的影响因素从理论上作了较全面的分析,为肽片段的缩合反应提供了一种新思路。
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Methods : The levels of fasting blood glucose and fast plasma insulin, the activities of superoxide dismutase and glutathione peroxidase and level of lipid peroxidate were measured in the pancreas, heart and liver of normal and streptozotocin-induced diabetes-prone mice exposed to multiple stressors for 6 weeks. Results : The FBG level in STZ mice was elevated by stress, while the FINS level was not increased.
用链脲佐菌素复制糖尿病倾向小鼠模型,予以6周慢性多相性应激,检测其空腹血糖、空腹胰岛素水平和胰、心、肝组织超氧化物歧化酶、谷肽甘肽过氧化物酶活性及脂质过氧化物含量。
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Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.
以3+2的合成路线使用α-胰凝乳蛋白酶和嗜热杆菌蛋白酶完成了N-保护的亮-脑啡肽的酶法全合成,并在第三戊醇中首次以嗜热杆菌蛋白酶为催化剂得到了Z-保护的亮-脑啡肽酰苯肼衍生物。
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The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin were analyzed by high performance capillary electrophoresis.
用高效毛细管电泳测定了重组人促红细胞生成素的微多相性、肽图,同时采用毛细管电泳质谱联用技术鉴定了部分非糖基化胰酶消解片段和O-126糖基化肽的结构。
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The results showed that: 1 SOD and GSH-PX activity of hepatopancreas and serum were significantly or extremely significantly increased when 5% or 10% alfalfa meal was added to the diet, whereas the MDA content of the two groups decreased. 2 The concentration of TC and TG in serum, hepatopancrac and muscle dropped as the amount of alfalfa meal increased.
结果显示,1添加5%和10%的苜蓿草粉能显著或极显著提高黄河鲤鱼血清和肝胰脏中超氧化物歧化酶和谷胱甘肽过氧化物酶活力,同时也能显著或极显著降低丙二醛含量,说明苜蓿草粉能有效地提高黄河鲤鱼的抗氧化能力。2血清、肝胰脏和肌肉中总胆固醇和甘油三酯含量都随着苜蓿草粉添加量的增加依次降低。
- 更多网络解释与胰肽酶相关的网络解释 [注:此内容来源于网络,仅供参考]
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erepsin:肠肽酶
[成分]主要含蛋白质、脂肪、甲壳质、糖类及蛋白质分解酵素. 蛋白质分解酵素为肠肽酶(erepsin)及胰元酶(trypsin).
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leucine aminopeptidase:亮氨酸氨基肽酶
有时患者血清中胰蛋白酶、亮氨酸氨基肽酶(leucine aminopeptidase)、淀粉酶等均增高,但由于其结果均不恒定,在临床诊断上不常应用. 有报告约80%~90%患者血清CEA检出阳性,但因CEA的特异性不强,亦不能作为诊断本癌的根据. 术前可以进行ERCP胰管细胞刷片或活检;
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pancreozymin:促胰酶素
CCK-PZ:是由十二指肠及空肠粘膜提取的一种肽类物质,它可刺激胰腺分泌大量胰液,称之促胰酶素(pancreozymin). 它与缩胆囊素(choletystokinin)相同,同时有收缩胆囊和松驰括约肌的作用,故称为缩胆囊素-促胰酶素(cholecystokinin-pancreozymin,
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pancreozymin:胰酶素
则在血浆中可以保持活性30~40小时,使大鼠的胰腺炎明显减轻.CCK-PZ:是由十二指肠及空肠粘膜提取的一种肽类物质,它可刺激胰腺分泌大量胰液,称之促胰酶素(pancreozymin).它与缩胆囊素(choletystokinin)相同,
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rennin:凝乳酶
由木瓜与凤梨中分别抽取出来的木瓜酵素(papain)及凤梨酵素(bromelin),均是分解蛋白质的酵素. 藉由水解作用,它们将蛋白质分解成较小的胜肽链. 其它分解蛋白质的酵素还包括胃蛋白酶(pepsin)、胰蛋白酶(trypsin)、凝乳酶(rennin)、胰酶(pancretin)及
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trypsin:胰朊酶
蛋白酶为肠肽酶(Erepsin)及胰朊酶(Trypsin). 性味咸、寒,清热解毒、消积食、健脾胃,主治消化不良、脘腹胀满、体倦无力、热病神昏、毒痢作呕、小儿疳积诸症;用量1.6~3.2g,虚寒者不宜.
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monomeric enzyme:单体酶
1.单体酶 单体酶(monomeric enzyme)一般由一条肽链组成,如溶菌酶、牛胰核糖核酸酶. 单体酶种类较少,一般多是催化水解反应的酶,相对分子量在(13~35) 103之间. 2.寡聚酶 寡聚酶(olimomeric enzyme)是由2个或2个以上亚基组成的酶,
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pancreatolipase:胰酯酶
pancreatin 胰酶(制剂) | pancreatolipase 胰酯酶 | pancreokinin 胰激肽
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E elastase:胰肽酶
7576 肌氨酸激酶MB同工酶 creatine kinase MB isozyme | 7580 胰肽酶E elastase | 7582 嗜中性胰肽酶E neutrophil elastase
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kallikrein kinin prostaglandin system KKPGS:激肽释放酶-激肽-前列腺素系统
kallidin KD 胰激肽 | kallikrein kinin prostaglandin system KKPGS 激肽释放酶-激肽-前列腺素系统 | kallikrein KK 激肽释放酶