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The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.
测定了它们对自由基的清除率和对四种细菌的抑菌效果,研究结果表明,总色素和褐藻黄素的浓度与自由基清除率成正比,当总色素浓度为0.52mg.ml~(-1)时,清除率达到65.819%,高于陆生植物黑米色素和紫甘薯色素;当褐藻黄素浓度为0.27mg.ml~(-1)时,自由基清除率达到19.85%,低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素;对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示,海带总色素对金黄葡萄球菌的抑菌活性最强,抑菌圈直径为18.9mm;褐藻黄素对八叠球菌的抑菌活性相对较强,抑菌圈直径为10.2mm。
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The biological effect of pUCP18/lasR was detected by RT-PCR, NAD method and the assay of pyocyanin.
RT-PCR检测LasB基因和LasI基因mRNA的表达,NAD法测定外毒素A的活性,紫外分光光度计测定绿脓菌素的产生水平。
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An example of such a mediator is pyocyanin (Mavrodi et al., 2001), produced by an isolated Pseudomonas aeruginosa species from the fuel cell.
这样斡旋人的例子是绿脓菌青素(等Mavrodi, 2001),生产由一个被隔绝的绿浓杆菌种类由燃料电池。
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The results indicate that the presence of GSH increased the expression of exoY、exoS、phzA1 and gacA,while no difference was observed with others virulence factor genes tested.PAO1 produced markedly less pyocyanin than the wild type strain PAOIP<0.01Treating with GSH, the PCN production of PAOI(△gshB was increased from 0.47μg/mL to 1.73μg/mL (P<0.01),the PCN production of PAO1 increased from 1.3μg/mL to 1.9μg/mL(P<0.05),this result indicates that GSH upregulates PCN production.
结果发现,GSH对启动子库中exoS、exoY、phzA1和gacA等致病性因子的表达有促进作用,对其余测试过的致病性因子则没有明显影响;铜绿假单胞菌分泌的重要致病因子绿脓菌素(pyocyanin,PCN)的含量,在PAO1中远低于PAO1P<0.01加入GSH后,PCN平均含量在PAO1(△gshB中从0.47μg/mL提高到1.73μg/mL,差异十分显著(P<0.01),在PAO1中从1.3μg/mL提高到1.9μg/mL,差异显著(P<0.05),表明GSH对铜绿假单胞菌生产PCN有促进作用。
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We beliee that, based on our analysis, the inclusion of a polymyxin in the initial, empirical antimicrobial regimen of an ICU patient with feer that is thought to be due to infection is justified in a hospital where P4 is approximately 0.5; that is, the probability that A.
基于我们的分析,我们相信感染性发热的ICU病人起初给予排除多粘菌素类的抗生素经验治疗被证明在P4大约为0.5的医院中,也就是鲍氏不动杆菌,绿脓杆菌和肺炎克雷伯菌仅对多粘菌素类敏感的概率大约为50%。
- 更多网络解释与绿脓菌素相关的网络解释 [注:此内容来源于网络,仅供参考]
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Escherichia coli:大肠杆菌
23、绿脓菌素(pyocyanin)测定用培养基(PDP琼脂)(1) 大肠杆菌(Escherichia coli) 取胆盐乳糖培养基3份,每份100ml,2份分别加入规定量的供试液,其中1份加入对照菌50~100个作阳性对照,第3份加入与供试液等量的稀释液作阴性对照.
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heteromorphic chromosomes:异形染色体
armature insulation 电枢绝缘 | heteromorphic chromosomes 异形染色体 | pyocyanolysin 绿脓菌溶素
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phenazine:吩嗪
它对许多抗生素具备很高的内在抗药性,并可产生许多致病因子,如碱性蛋白酶(alkalineprotease)和吩嗪(phenazine)等,是难以治疗的病原菌. 绿脓菌素作为吩嗪的一种衍生物,不仅是一种致病因子,更重要的是作为铜绿假单胞菌中的一种信号分子.
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abortin:流产菌素
链球菌素 streptocin | 流产菌素 abortin | 绿脓[菌]素 pyocyanin
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pyocyanin:绿脓(菌)素
配制其它糖、醇发酵培养基时,将各种糖、醇分别配成l0%溶液,与基础液同时灭菌. 以无菌操作将5ml糖、醇溶液加入100ml基础液内,分装于灭菌小试管中. 1.26 绿脓菌素(pyocyanin)测定用培养基(PDP琼脂)1.34 哥伦比 (Columbia)琼脂培养基
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pyocyanin:绿皮菌素
pylorus 幽门 | pyocyanin 绿皮菌素 | pyocyte 脓细胞
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pyocyanin:绿脓菌色素,脓绿素
"绿脓菌抗毒素","Pyocyaneus antitoxin" | "绿脓菌色素,脓绿素","Pyocyanin" | "绿脓菌溶血素","Pyocyanlysin"
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pyocyte:脓细胞
pyocyanin 绿皮菌素 | pyocyte 脓细胞 | pyramid 锥体
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polymyxin B:多粘菌素
(7)复方多粘菌素B和磷霉素钠滴耳剂(又名耳炎灵);每毫升含多粘菌素(polymyxin B)l万u、磷霉素钠30mg(phosphomycin sodium)、地塞米松0.1mg等成分. 作用 有强大的抗菌作用,对G-杆菌和绿脓杆菌的作用显著. 对金葡菌、肺炎链球菌均有抗菌作用.