细胞基因

基本解释 (translations)

cytogene

与细胞基因相关的网络例句 [注：此内容来源于网络，仅供参考]

Methods: Human osteoblasts were cultured on flexible-bottomed plates and subjected to 8% elongation by stress unit at 6 cycles/min (i.e. 5-s elongation and 5-s relaxation) for 24 hours in the experimental groups. Control group did not exert any stress.

通过体外细胞加载系统对培养的人成骨细胞施加8%形变率、6周／min的周期性牵张力24 h，应用基因表达谱芯片技术对人成骨细胞中mRNA表达水平进行对比杂交分析，检测周期性牵张力加载组成骨细胞基因表达谱的变化。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Crocin can induce the significant alteration of gene expression profile of T24 cell. It is suggested that the widly konwn anti-tumor effects of crocin are medicated at least in part by regulating the cell cycle controlling gene expression.

藏花素可以诱导T24细胞基因表达谱广泛的改变，并可能通过调控细胞周期相关基因的表达来抑制T24细胞的增殖。

OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34+ and CD133+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34+ and CD133+ hematopotic stem cells/hemapoietic progenitor cells in vitro.

目的：利用基因芯片比较脐血CD34+和CD133+细胞基因表达的差异，并探讨碱性成纤维细胞生长因子体外诱导脐血CD34+和CD133+造血干/祖细胞分化的基因表达变化，及其对碱性成纤维细胞生长因子反应性差异。

Intracytoplasmic sperm injection, which is a new technique of embryo engineering of domestic animals, can solve the problem of porcine polyspermy.

用GFP基因转染的精子注射后化学激活的ICSI卵母细胞基因表达率显著高于电激活处理的ICSI卵母细胞的基因表达率。

Objective: The purposes of the present study are as follows: 1 To study the effect of direct contact with VSMC on transdifferentiation of MSC; 2 To construct MSC of Dual-stable expression of AT2R gene medicated by tetracycline regulatable system.; 3 To investigate the effects of AT2R gene medicated by tetracycline regulatable system on neointimal hyperplasia in rat carotid arteries after balloon angioplasty.

本实验体外观察MSC与VSMC在直接接触培养下的分化情况，并以MSC为载体，通过体外细胞基因转染及筛选，获得双重稳定受到强力霉素调控表达AT2R基因的MSC，将此受到调控表达的AT2R基因导入大鼠颈动脉球囊损伤动物模型中，观察MSC细胞移植实现AT2R基因在体可调控表达在新生内膜中形成的作用及潜在意义。

Meanwhile, in order to explore the stability of gene expression and the properties of gene-modified bone marrow cells further, we have observed the capability of reconstituting hematopoietic system of gene-modified and the expression of transduced gene in vivo by using of BMT to engraft lethally irradiated mouse with the gene-modified bone marrow cells.

同时，为进一步探讨转导细胞基因表达的稳定性以及生物学特征，本实验通过骨髓移植的方法，将基因标记骨髓细胞移植给受致死量照射小鼠，观察了基因转导细胞重建体内造血的能力，以及造血重建后外源基因在脾结节及骨髓细胞的表达。

We used R1 cell line, primary established by Nagy in Canada, as ES cell to establish culture environment for ES cell, and successfully subculture R1 cell with LIF and gelatinized dishes and observed its growth characteristic.

同时，也初步建立包括129小鼠基因组文库筛选获得基因组DNA片段、基因组DNA结构分析、基因打靶载体构建、ES细胞体外培养、电穿孔基因转移、重组事件的药物选择和分子鉴定在内的ES细胞基因打靶技术。

与细胞基因相关的网络解释 [注：此内容来源于网络，仅供参考]

cytogene：细胞基因 细胞质基因

cytoflavin细胞黄素 | cytogene细胞基因 细胞质基因 | cytogenesis细胞发生

house-keeping gene：管家基因

细胞内与分化有关的基因按功能可以分为两类:一类是管家基因(house keeping gene),它是为维持各种细胞基本活动所必需的结构和功能蛋白质编码的基因,这种基因是各种类型细胞所共有的,如为各种参与细胞分裂和能量代谢的蛋白质编码的基因.

house-keeping genes：管家基因

研究显示,一些真核细胞启动子具有延长和调节靶细胞基因表达作用,从而被称为管家基因(house keeping genes),但真核细胞启动子的表达水平一般都低于病毒启动子.

housekeeping gene：看家基因

其中某些基因表达产物是细胞或生物体整个生命过程中都持续需要而必不可少的,这类基因可称为看家基因(housekeeping gene),这些基因中不少是在生物个体其它组织细胞、甚至在同一物种的细胞中都是持续表达的,可以看成是细胞基本的基因表达.

retinoblastoma：成视网膜细胞瘤

RB基因于70年代最先在成视网膜细胞瘤(retinoblastoma)中被发现,并于1980年由Friend等[1]克隆出其所在的DNA片段. RB基因是目前人们较普遍接受并作了较深入、广泛研究的抑癌基因之一. 现有研究表明,RB基因是细胞周期控制及细胞分化中重要的调控因子[2].

retinoblastoma：视网膜胚细胞瘤

这些基因依其在受感染细胞中表现的先后顺序而分为早期基因与晚期基因,L1、L2等两个晚期基因负责病毒壳体的蛋白质,早期基因E6、E7所制造的蛋白质为主要的致癌蛋白质,分别与p53以及视网膜胚细胞瘤(retinoblastoma)家族之蛋白质结合并

suicide gene：基因

将某些细菌及病毒中特有的药物敏感基因转导入肿瘤细胞,使肿瘤细胞产生某些酶类,将原来无毒的抗病毒药物或化疗前体药物代谢转化成细胞毒性产物而杀伤宿主细胞,这种使肿瘤细胞自杀的基因称为"自杀基因"(suicide gene).

cytogenic damage：细胞基因损伤

Cortex magnoliae officinalis厚朴 | cytogenic damage细胞基因损伤 | damage损伤,损害,毁坏

transforming gene：转化基因

并在特定情况下它们在细胞中的变异或异常2.上述肉瘤和急性白血病基因加上腺病毒的Ela、Elb区域,以及乳多孔病毒(Papovavirus)的初期基因区域等,虽非来源于细胞的基因,但含有使细胞致癌的基因,属于广义的致癌基因,也称为转化基因(transforming gene).

oncogene：癌基因,原癌基因

原癌基因 原癌基因(oncogene)是细胞内与细胞增殖相关的基因,是维持机体正常生命活动所必须的,在进化上高等保守. 当原癌基因的结构或调控区发生变异,基因产物增多或活性增强时,使细胞过度增殖,从而形成肿瘤. 原癌基因的产物主要包括(表13-2,