组织切片术

基本解释 (translations)

histotomy

与组织切片术相关的网络例句 [注：此内容来源于网络，仅供参考]

Materials and Methods: From January of 2007 through December of 2007, 6 patients underwent subcutaneous liposuction with shaving for bromhidrosis.

目的与目标：藉由病理组织切片观察顶浆腺的数量来评估皮下脂肪抽取术结合旋转刀刮除术能否有效治疗狐臭。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

A firm tumor was identified adjoining the capsule of the superficial lobe of the parotid gland. Both frozen section and pathologic reports showed histiocytic necrotizing lymphadenitis.

术中发现紧邻腮腺浅叶被膜有一坚实、大小3×3cm的肿瘤，冰冻切片及永久切片报告均为组织球性坏死性淋巴结炎。

Pre-operative diagnostic methods included detailed ophthalmologic examination, coronary CT scan of orbit, and tissue biopsy.

术前诊断方法包括详细眼科学检查、电脑断层扫描及组织切片等。

Most techniques in vivo monitoring the distribution, prolification and migration of transplanted cells. To objectively evaluate curative effect after cells transplantation, most techniques for the study of stem cell transplantation in animal models require histological analysis to determine the fate and migration of cells.

监测活体内移植入细胞的分布、增殖、迁移的方法虽然有多种，但传统方法都需要在离体状态下进行组织学切片分析和鉴定，无法准确地观察植入细胞的生长分化情况，从而难以对干细胞移植术的疗效作出客观评价。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

与组织切片术相关的网络解释 [注：此内容来源于网络，仅供参考]

Image analysis：图像分析术

在组织切片上显示某种物质的存在和分布状态即核酸分子杂交组织化学术,检测基因(DNA片段)的有无,基因的表达活性(mRNA)图像分析术(image analysis)又称形态计量术(morphometry):应用数学和统计学原理对组织切片提供的平面图像进行分析,

microtomy：组织切片术

microtome knife holder 切片机刀架 | microtomy 组织切片术 | microtonometer 微测压计

histotomy：组织切开术

histotome 组织切片器 | histotomy 组织切开术 | histrionic 戏剧的

histotomy：组织切片术

histotome 组织切片机 | histotomy 组织切片术 | hollow director 有槽探子

组织学切片fixation n.f. 固定：coupe histologique n.f

microscopie optique n.f. 光学显微镜(术) | coupe histologique n.f. 组织学切片fixation n.f. 固定 | inclusion n.f. 包埋