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RESULTS: The collapse was obvious at weeks 2 and 6 after modeling, with only few granulation tissues and fibrous tissues. No chondrocytes were observed. At weeks 12 and 18, fibrous tissues were found at the defect site, and bone tissue and cartilage proliferation was observed at the bottom and margin. However, it was greatly different from normal cartilage. Moreover, there was still no chondrocytes.
结果:实验兔后腿关节的股骨髁关节面的关节软骨缺损后,在制模后2,6周缺损部位凹陷明显,只有少许肉芽组织和纤维组织生长填充,未见软骨细胞爬行生长,12,18周见缺损部位以纤维组织增生为主,底部及边缘可见骨组织和软骨增生,但是镜下形态仍与正常软骨组织区别较大,表面仍未见软骨细胞爬行生长。
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The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, which neans tuberculous restoration,and scar formation.
炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特征;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。
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The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells,caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells,and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue,cells and mucous oweing to hardness to get puncture,which neans tuberculous restoration,and scar formation.
炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见量坏死组织及碎屑、少残碎不全类上皮样细胞,此期主要能查到抗酸菌特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。
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The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, which neans tuberculous restoration,and scar formation.
炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。
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The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, neans tuberculous restoration,and scar formation.
结核初期-炎性增殖期60例,占5.5%;结核早期-淋巴结节期130例,占11.9%;结核中期-结核性结节期有590例,占54.1%;结核晚期-干酪样脓样坏死期有280例,占25.7%;结核恢复期-纤维素增殖期30例,占2.8%。炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。
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Results Among the 52 cases, 30 cases were confirmed to fibrosarcomas, which were identical with primary dingnoses;22 cases were revised to malignant fibrous histocytoma (8 cases), malignant nerve sheath tumor (4 cases), monophasic spindle cell synovial sarcoma (3 cases), leiomyosarcoma(3 cases), rhabdomyosarcoma(2 cases) and abdominal desmmoid tumor (2 cases). Of the 18 cases, only two cases were misdiagnosed and were revised to malignant fibrous histocytoma and spindle cell synovial sarcoma, respectively.
结果:1980年前52例中维持原诊断30例,修改诊断22例(42.3%),分别为恶性纤维组织细胞瘤8例、恶性神经鞘膜瘤4例、单相性梭形细胞滑膜肉瘤3例、平滑肌肉瘤3例、横纹肌肉瘤2例、韧带状纤维瘤2例;1980年后纤维肉瘤18例中,维持原诊断16例,修改诊断2例(11.1%),分别为恶性纤维组织细胞瘤和滑膜肉瘤各1例。
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Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.
结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。
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Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.
目的:探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率,分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响;方法:69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究;并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变;分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响;结果:15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生,所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁;12例PV、4例ET、4例IMF中检测到JAK2V617F;有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L,比较不同纤维增生程度的PV、ET患者的白细胞数均值,P值为0.367。
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HE staining revealed that collagen fiber, fibroblasts and granulation tissue and synechia belt around tendons and stomas were apparently less in contrast to control group.
术后4周苏木精-伊红染色结果表明,实验组腱吻合口被胶原纤维连接,腱周及吻合口周围胶原纤维、成纤维细胞及肉芽组织明显较对照组少,腱周纤维组织较对照组粘连轻,粘连带稀少。
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A 46-year-old man presented with a gradually growing nodule on the left dorsal foot, which had been present for over 10 years. In the beginning, the lesion was treated as a keloid. Because of poor response to treatment, biopsy was arranged. Sections showed a dermal tumor composed of numerous foamy cells, hyalinized wiry collagen, spindle cells and entrapped collagen. The foamy cells were positive for KP1(CD68) and negative for S-100 protein and HMB-45. The histological and immunohistochemical results were consistent with the diagnosis of lipidized fibrous histiocytoma.
一位46岁男性从十年前开始於左足背上出现一个逐渐长大之结节,一开始被当作蟹足肿给予治疗,因为对治疗的反应不好,故安排了切片检查,病理显示一个由大量泡沫状细胞,坚硬而透明之胶原纤维,梭状细胞,胶原纤维束所构成的真皮肿瘤,免疫组织化学染色显示KP1(CD68)阳性,S-100 protein与HMB45阴性,综合病理与免疫组织化学染色结果,诊断为脂肪性纤维组织细胞瘤。
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fibrosarcoma:纤维组织: 纤维肉瘤
fetoprotein; AFP 胎蛋白 | fibrosarcoma 纤维组织: 纤维肉瘤 | FIGO: International Federation of Gynecology and Obstetrics 国际妇产科学联盟
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fibrositis:纤维组织炎
骨髓纤维变性 fibrosisfibrosis,bone marrow | 纤维组织炎 fibrositis | 纤维变性的,纤维组织生成的 fibrotic
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fibrositis:纤维组织炎 (名)
fibrosarcoma 纤维肉瘤 (名) | fibrositis 纤维组织炎 (名) | fibrotic 纤维化的 (形)
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fibrous structure:纤维组织
fibrous insulant 纤维绝缘材料 | fibrous structure 纤维组织 | fictitious force 虚拟力
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collagenous tissue:胶原组织, 白纤维组织
collagenous fibril | 胶原纤丝 | collagenous tissue | 胶原组织, 白纤维组织 | collagenous | 成胶的, 产生成胶的
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endocardial fibroelastosis:心内膜弹性纤维组织增生
endocardial electrode 心内膜电极 | endocardial fibroelastosis 心内膜弹性纤维组织增生 | endocardial fibrosis 心内膜心肌纤维化
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fibromatosis:瘤样纤维组织增生
瘤形成neoplasia | 瘤样纤维组织增生fibromatosis | 瘤状的tuberculate
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fibroplastic proliferation:纤维组织增生
fibromuscular dysplasia of cerebral artery 脑动脉纤维肌肉发育异常 | fibroplastic proliferation 纤维组织增生 | fibrosarcoma of bone 骨纤维肉瘤
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fibrotic:纤维变性的,纤维组织生成的
纤维组织炎 fibrositis | 纤维变性的,纤维组织生成的 fibrotic | 纤维性 fibrous
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inogenesis:纤维组织形成
inogen 肌收缩原 | inogenesis 纤维组织形成 | inoglia 纤维胶质