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Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.
抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞,导致细胞生长的抑制和凋亡罗伯托利,斯特凡Purgato ,苏埃洛卡默琳,拉菲尔Fronza ,梅奥尼博洛尼亚,西蒙娜Alboresi ,莫妮卡弗兰索尼,罗伯托Corradini ,斯特凡诺斯福尔扎,安德烈Faccini ,杰森先生Shohet , Rosangela Marchelli ,和安德烈Pession儿科,博洛尼亚大学,圣' Orsola -马尔皮基医院,博洛尼亚,意大利;有机部和工业大学化学帕尔马,帕尔马,意大利和3Center的细胞和基因治疗,德州儿童癌症中心, Baylor医学院,得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞,针对一个独特的序列反义DNA链的第2外显子,并与在MYCN 其氨基端为核定位信号肽。