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The RAPD system of Gladiolus was optimized. Of the 12 random primers analyzed in this research, 9 primers can show steady and polymorphic amplification profiles.
建立了适用于唐菖蒲RAPD分子标记的反应体系，从12条随机引物中筛选出9条可扩增出多态性条带的引物。
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With this RAPD condition, the bulked segregant analysis was used toidentify RAPD markers differentiating strains avirulent and virulent on Tsuyuake.
利用这一体系和集群分离分析方法从420个随机引物中，筛选出2个引物能扩增出与Avr-Tsuyu连锁的2个标记：〓和〓。
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The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.
利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。
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In screens 20 from 100 10bp along with the machine-drawn to increase the effect to direct the thing well to carry on increases, altogether has 188 bandings,118 are the polymorphism belt, accounts for 62.8%, average each DNA polymorphism belt number which directs the thing to increase is 5.90, has promulgated the sweet potato variety heredity multiplicity; construct the shape chart called UPGMA to reflection variety diverse , will supply to try 31 variety divisions will be 4 broad headings, has promulgated between Hunan sweet potato variety from the molecular level and with the foreign country variety between sibship.
从100个10bp的随机引物中筛选出20个扩增效果较好的引物进行扩增，共产生188条带，其中118条为多态性带，占 62.8%，平均每个引物扩增出的DNA多态性带数为 5.90条，揭示了甘薯品种的遗传多样性；根据扩增结果构建出反映品种多样的UPGMA聚状图，将供试31个品种划分为4大类，从分子水平上揭示了湖南甘薯品种之间及其与外域品种之间的亲缘关系。
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Genetic diversity among 7 populations of Trillium tschonoskii were analyzed by using inter SSR and amplified fragment length polymorphism molecular markers. The results were as follows:(1)ISSR: 12 out of the 100 primers showed consistent banding patterns and amplification, a total of 135 bands were produced with an arrange of 250-2500bp, 46 of which (34.07%) were polymorphic.
应用锚定SSR和限制性片段多态对7个延龄草居群的遗传多样性进行了分析，结果如下：从100个引物中筛选出12个ISSR引物，在250-2500bp之间共扩增出的135个条带，其中多态性带为46，占34.07％。
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Fourteen wampee cultivars collected from Guangdong and Guangxi were used for RAPD analysis in this study. 16 primers selected from 150 random primers were used to amplify 87 DNA fragments and 57 bands (54.0%) showed polymorphisms.
摘 要：对14个黄皮品种进行 RAPD 分析，从150个随机引物中筛选出16个能在种质间表现出多态性的引物，共扩增出 87 条谱带，其中多态性带47条，多态性比例为54.0%。
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Fingerprints of broiler chickens divergently selected for very low den sity lipoprotein in fat line and lean line of the third generation were studied using inter simple sequence repeated technique. Geneti c polymorphism and genetic similarity coefficient between two groups were analyze d. Ten primers were screened from 60 SSR primers and 62 sites were deprived. Of which 39 bands were polymorphic sites and the frequency was 63 percent.
通过对初步进行血浆极低密度脂蛋白双向选择的三世代高、低脂群肉鸡进行ISSR指纹分析，评定其遗传多态性及两群体间的遗传相似系数。60个引物筛选出10个引物用于检测分析；共扩增出62条谱带，其中39个是多态性片段，多态率为63%。
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The average percentage of polymorphic bands are 72.06% for ISSR and 66.67% for RAPD respectively. The genetic diversity of ginkgo revealed by ISSR (the average effective number of alleles is 1.8780, the average Nei"s gene diversity is 0.4652, and the average Shannon"s information index is 0.6574) are all higher than those by RAPD (the average effective number of alleles is 1.7842, the average Nei"s gene diversity is 0.4317, and the average Shannon"s information index is 0.6211), while the standard errors of the parameters estimated by ISSR were lower. Therefore, ISSR molecular markers are more suited than RAPD molecular markers when testing the genetic diversity of ginkgo populations and determining the genetic relationship of among populations or among individuals which are much similar hereditarily.
本文应用RAPD和ISSR两种分子标记技术，选取中国5个可能的野生银杏居群共计75个样品，对其遗传多样性进行了研究，得出以下结论：(1)用筛选出的12个RAPD引物和10个ISSR引物进行PCR扩增，分别扩增出65条和68条重复性高、清晰的条带，多态性位点百分率分别66.67%和72.06%，ISSR揭示的银杏遗传多样性(平均有效等位基因数目为1.8780，平均基因多样度为0.4652，平均信息指数为0.6574)高于RAPD所得到的结果(平均有效等位基因数目为1.7842，平均基因多样度为0.4317，平均信息指数为0.6211)，其所估算参数的标准差要低于RAPD所估算出的值，因此，在研究亲缘关系非常近的银杏物种的遗传多样性并试图确定居群间或个体间的遗传关系时，ISSR分子标记技术比RAPD分子标记技术更为合适。
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ResultsThe major occupational hazardous factors in the project were coke dust, carbon monoxide, benzopyrene, benzene soluble matter and noise. The detection results showed that MAC of benzopyrene on coke pushed side, TWA of benzene soluble matter of two posts and noise level of coke griddle were overproof the rest were eligible.
结果 该扩建项目主要职业病危害因素是焦炭尘、一氧化碳、苯并芘、苯溶物、噪声等；检测结果示焦侧苯并芘的最高接触浓度、炉盖工、出炉工苯溶物的时间加权平均浓度及输焦振筛的噪声强度超标，其余检测点检测结果均符合国家职业卫生标准。
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The present invention is one comprehensive innocuous city solid refuse treating system. In the system, the city solid refuse is first stoved, sterilized and sorted, then magnetically separated to separate out iron containing waste, and finally sieved into the undersize fed to one mixing tank and the oversize further sorted, with the rest matter being crushed and fed also to the mixing tank for fermentation to produce organic fertilizer.
城市固体垃圾无害化、资源化、再生综合利用处理系统，首先将含有大量水分的城市固体垃圾进行烘干、杀菌消毒，进行初级分选，把织物纸类、玻璃、砖头瓦块、废塑料、废橡胶类、废电池、木质类等分拣出来，进入磁选分检出废铁制品，再进入分级筛，筛下物被输送到一个预混罐，筛上物进行再次分选，剩余的垃圾粉碎后也被送入预混罐，在预混罐中加入发酵菌进行混合固体发酵，发酵后成为有机肥。
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Zingiber officinale：姜
摘要 利用RAPD技术对20个姜(Zingiber officinale)品种进行了遗传多样性的研究. 从200多个引物中筛选出25个有效引物,共扩增出171条DNA带,其大小分布在100~3 500 bp之间,其中111条为多态性,约占总数的64.91%,平均每个引物的扩增带为6.8条.