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It is very difficult to isolate chymopapain from the latex of Carica papaya because chymopapain is very similar to the other three cysteine proteinases in the latex at the structure and properties.
由于番木瓜乳汁中含有的四种巯基蛋白酶在结构、性质等方面十分相似,因而分离木瓜凝乳蛋白酶比较困难。
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Methods Using means of collection, 20% and 40% saturated ammonium sulfate fractionation, SP-sephadex C 50 column chromatography and hydroxyapatite chromatography to purify papain from Carica papaya.
采用初步采集处理,20%,40%硫酸铵分级沉淀、SP-sephadex、C50柱色谱、羟基磷灰石柱色谱,从番木瓜乳汁中分离纯化木瓜蛋白酶。
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Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.
方法从生番木瓜中提取mRNA后进行反转录,得到木瓜凝乳蛋白酶的cDNA,PCR产物克隆到pET22-b载体中,重组质粒转化至大肠杆菌BL21,进行诱导表达及表达产物的检测。
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papain:木瓜蛋白酶
木瓜蛋白酶:(Papain)简称木瓜酶,又称为木瓜酵素. 是利用未成熟的番木瓜(Carica papaya)果实中的乳汁,采用现代生物工程技术提炼而成的纯天然生物酶制品. 对动物的蛋白纤维(毛)有较强的水解能力
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papain:木瓜酶
木瓜酶(Papain)全称木瓜蛋白酶. 是利用未成熟的番木瓜果(Caricapapaya)表皮的白色乳汁,采用现代生物工程技术提炼而成的纯天然生物酶. 它是一种含巯基(-SH)肽链内切酶,具有蛋白酶和酯酶的活性,有较广泛的特异性,
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papain peptidase I:木瓜肽酶
papain 木瓜酶;番木瓜蛋白酶 | papain peptidase I 木瓜肽酶I | papain peptidase II 木瓜肽酶II
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papayotin:番木瓜汁;番木瓜酶,番木瓜蛋白酶
papaya番木瓜树;番木瓜汁;番木瓜 | papayotin番木瓜汁;番木瓜酶,番木瓜蛋白酶 | chymopapain 木瓜凝孔蛋白酶
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papaya; melon tree; Carica papaya L:番木瓜;万寿果;木瓜(俗)
派朴果 papaw; Asimina triloba Dunal. | 番木瓜;万寿果;木瓜(俗) papaya; melon tree; Carica papaya L.; | 番木瓜素;番木瓜蛋白酶 papayin; apain