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The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.
检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性，最低可以检测到10个TCID50/100μl的病毒，说明该方法的有较好的特异性和敏感性。
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In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.
本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。
- 更多网络解释与狂犬病状的相关的网络解释 [注：此内容来源于网络，仅供参考]
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heat apoplexy：热射病
如脑炎(cephalitis)、脑膜炎(cephalitis meningica)、日射病(heliosis)、热射病(heat apoplexy)、中毒(intoxation)及某些传染病的初期. 如同时伴有踢咬和攻击人畜,则是狂犬病地特征. 典型的木马状站立姿势:呈头颈平伸、肢体僵硬、四肢关节不能屈曲、尾根挺起、鼻孔开张、瞬膜露出、牙关紧闭等形象,
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Rabies virus：狂犬病病毒
病原 狂犬病病毒(Rabies virus)属于弹状病毒科(Rhabdoviridae)的狂犬病病毒属(Lyssavires). 病毒的核酸为单股RNA,病毒含有一种糖蛋白(GP),一种核蛋白(NP)和两种膜蛋白(M1和 M2). GP是一种跨膜糖蛋白,构成病毒表面的纤突,
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rabiform：狂犬病状的
rabieshydrophobia 恐水病 | rabiform 狂犬病状的 | race-base 种族立场