- 更多网络例句与牛舌相关的网络例句 [注:此内容来源于网络,仅供参考]
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ObjectiveTo optimize the extraction process of total flavonoids in Anchusa italica Retiz.
目的研究维吾尔药牛舌草中总黄酮的最佳提取工艺。
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Any of several usually hairy Old World plants, especially in the genera Anchusa, Brunnera, and Echium, having blue or violet flowers.
牛舌草一种多毛的东半球植物,尤指牛舌草属和蓝蓟属,有蓝色或紫色的花
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objectiveto optimize the extraction process of total flavonoids in anchusa italica retiz.methodsthe content of total flavonoids was determined by uv spectrophotometer.the influence of each factor was measured by orthogonal experiments.
目的研究维吾尔药牛舌草中总黄酮的最佳提取工艺。方法采用正交实验确定各因素的影响大小,确定维吾尔药牛舌草中总黄酮的最佳提取条件,通过紫外分光光度法测定总黄酮含量。
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Any of various hairy plants of the Eurasian genus Anchusa, having blue or violet flowers grouped on elongated cymes.
牛舌草欧亚牛舌草属多种毛状植物的任一种,其蓝色或紫色的花朵聚集于细长的聚伞花序上
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Zhangzhishan's son , liked bull's tongue cake most . He must left dregs only . At that time , most children were greedy , although all of the cakes were eaten by him , my feeling is sorrow .
当年住在天坛南里的张志善的儿子最爱吃的就是牛舌饼,这小子,每次都给我们剩下一对渣,那个时候孩子们缺嘴,都给他吃了,我心里还是挺难受呢。
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Bull's tongue is gigantic but the key point is not the weight and height. It cannot be believed that sturdy fiber accompanying with soft texture contrasts sharply with its huge body, quite different from Fu Shan Korean food and Japanese-Korean roast restaurant.
牛舌巨大,但关键不是个头,貌似牛排一般厚实硕大的身材里居然是粗壮的纤维质伴随松软的口感,和釜山料理或是什么日韩的烧烤味道真的是完全不同啊。
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Steak mushroom bacteria, also known as tongue, liver fungus, pig tongue fungus, Japan said Velvet liver, blood Velvet, Mushroom soft flesh, juicy, red-brown, short-throw type or not, flavor concentration, can be used as medicine, the role of therapeutic blood.
牛排菇 又名牛舌菌、猪肝菌、猪舌菌、日本称肝脏茸、鲜血茸,菇肉质松软、多汁、红褐色、短柄式或无、香味浓,可以入药,食疗补血作用。
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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.
为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株;随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。
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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.
为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株:随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。
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The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.
研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术,研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异,初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带:晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合,分别为SF25*SR2和SF3*SR26;晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14;NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14,烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合,研究了普通烟草不同类型间的亲缘关系,将普通烟草类型间8个不同供试材料划分为3类别。I:烤烟类型G-28II:白肋烟类型(MD40、By21、大白筋599III:香料烟类型(Basma、Sumsun、小牛舌、千层塔)。
- 更多网络解释与牛舌相关的网络解释 [注:此内容来源于网络,仅供参考]
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alkannin;alkanin;alkanna-red:牛舌草红质
牛舌草属(紫草科) Alkanna;Anchusa | 牛舌草红质 alkannin;alkanin;alkanna-red | 黑尿症;夺碱质尿症 alkaptonuria;alcaptonuria
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Alkanna;Anchusa:牛舌草属(紫草科)
烷属烃;烷属碳化氢 alkane | 牛舌草属(紫草科) Alkanna;Anchusa | 牛舌草红质 alkannin;alkanin;alkanna-red
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Anchusa capensis Thunb:好望角牛舌草
Anchusa azurea Mill. 'Loddon Royalist' 天蓝牛舌草'伦敦保皇党' | Anchusa capensis Thunb. 好望角牛舌草 | Anchusa capensis Thunb. 'Blue Angel' 好望角牛舌草'蓝天使'
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Tongueoxin aspic:牛舌冻
牛肉杂碎=Beef chop suey | 牛舌冻=Tongueoxin aspic | 牛舌多士=Tongue toast
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Curry oxtongue RMB:和牛上等咖喱牛舌
和牛上等厚切盐烤 Roasted thick meat with salt RMB 180 | 和牛上等咖喱牛舌 Curry oxtongue RMB 180 | 秘制和牛牛舌 House secret oxtongue RMB 88
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House secret oxtongue RMB:秘制和牛牛舌
和牛上等咖喱牛舌 Curry oxtongue RMB 180 | 秘制和牛牛舌 House secret oxtongue RMB 88 | 和牛肝 Liver RMB 40
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Roasted oxtongue withy salt RMB:和牛上等盐烤牛舌
椒盐京葱沫牛小排 Salted beef steak with scallion RMB 98 | 和牛上等盐烤牛舌 Roasted oxtongue withy salt RMB 180 | 和牛上等厚切盐烤 Roasted thick meat with salt RMB 180
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Anchusa azurea Mill. 'Loddon Royalist:天蓝牛舌草'伦敦保皇党
Anchusa azurea Mill. 'Feltham Pride' 天蓝牛舌草'费尔瑟姆' | Anchusa azurea Mill. 'Loddon Royalist' 天蓝牛舌草'伦敦保皇党' | Anchusa capensis Thunb. 好望角牛舌草
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Fistulina:牛舌菌属
18.Fistulinaceae牛舌菌科 | Fistulina牛舌菌属(179) | 19.Bondarzewiaceae瘤孢多孔菌属
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Fistulina:(六十二)牛舌菌
(六十一)耳匙菌类Auriscalpium | (六十二)牛舌菌Fistulina | (六十三)假牛舌菌Pseudofistulina