淋巴细胞增殖的

基本解释 (translations)

lymphopoietic

与淋巴细胞增殖的相关的网络例句 [注：此内容来源于网络，仅供参考]

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

The effects of Cortex phellodendri alkaloid on splenic lymphocyte proliferation after heat stress were detected by MTT chromatometry.On the basis of the above detection,CPAs extracted by limewater,ethanol,sulfuric acid water and the mixture of sulfuric acid-and-ethanol were choosen.

采用MTT法测定了热应激后小鼠脾淋巴细胞增殖的情况，以此作为醇提、碱提、酸醇提、酸提4种提取方法所得黄柏生物碱的筛选依据；采用ELISA法测定了醇提黄柏生物碱对热应激后小鼠脾淋巴细胞IFN-γ、IL-4分泌水平的动态影响。

Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenodide-Ro on the production of cyrokines interleukin-2 (IL-2), interferon-γ and interleukin-4 (IL-4) from mutine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of TH1 cytokine IFN-γ and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction analysis.

方法[3H] TdR参人法检测人参皂有－Ro对小鼠脾淋巴细胞增殖的影响；酶联免疫吸附法检测人参皂苷－Ro对小鼠脾淋巴细胞产生细胞因子白介素－2、干扰素－γ和白介素-4的影响；逆转录聚合酶链式反应分析法研究人参皂苷-Ro对小鼠脾淋巴细胞引干扰素－Υ、白介素-4mRNA表达的影响。

Igniarius were carried out. It will be of scientific significance in researching and developing rare medicial fungi and be useful for searching new natural antitumor medicine.The crude polysaccharides of P. igniarius PIP30、PIP60、PIP80 and PIWE1-10、PIWE10-100、PIWE100 were obtained by ethanol precipitation and ultrafiltrate respectively after hot water extracting.

首先对桑黄子实体多糖进行了分离纯化，通过热水提取、乙醇分级沉淀分离得到粗多糖PIP30、PIP60和PIP80，通过超滤分离得到粗多糖PIWE1-10、PIWE10-100和PIWE100；6种粗多糖经体外刺激淋巴细胞增殖实验表明均有体外刺激淋巴细胞增殖的作用。

Allergenic mixed T lymphocytes reaction was used to observe the influence on leukomonocyte proliferation.

以混合淋巴细胞反应强度观察树突状细胞对淋巴细胞增殖的影响。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

Allogeneic and syngeneic fresh bone, autolyzed antigen-extracted bone, bone matrix gelatin, demineralized bone matrix were implanted into the muscle pouch of mice, at different times after implantation, the immunological, histological and alkaline phosphatase assay were conducted. The results revealed that all four kinds of allogeneic implants activated specific cellular and humoral immune responses, most notably in fresh bone group, the AAA, BMG and DBM inhibited the proliferation of the lymphocytes in vitro and BMG had the most powerful inhibiting action, allogeneic AAA, BMG and DBM might induce heterotopic osteogenesis in vivo, however, there were obvious difference in ALP and histomorphometry between allografts and isografts.

在小鼠肌肉内植入同种异体或同系新鲜骨、自消化抗原去除骨、骨基质明胶、脱钙骨基质，术后免疫学、碱性磷酸酶、组织学检查发现：4种同种异体植入物均可引发特异性细胞免疫反应和体液免疫反应，其中以新鲜骨移植引发的免疫反应最为显著；自消化抗原去除骨、骨基质明胶、脱钙骨基质在体外具有抑制淋巴细胞增殖的作用，骨基质明胶对淋巴细胞增殖的抑制作用最显著；同种异体与同系4种植入物在体内成骨活性有显著差异；自消化抗原去除骨、骨基质明胶、脱钙骨基质均有异位诱导成骨活性，其中骨基质明胶骨诱导活性最佳。

Objective To investigate the effects of aloe-emodin on PHA-induced proliferation and intracellular Ca2 mobilization of T lymphocytes.Methods The proliferation of T cell was tested by MTT assay.

目的研究芦荟大黄素对植物血凝素引起的大鼠T淋巴细胞增殖和细胞内Ca2 浓度([Ca2 ]i)变化的影响，探讨芦荟大黄素对T淋巴细胞增殖的作用和机制。

ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.

目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

与淋巴细胞增殖的相关的网络解释 [注：此内容来源于网络，仅供参考]

duct：管

因为通常是将分泌物引至体表和体内的管腔中,所以附有排出管--导管(duct). 这样的腺体称外分泌腺. 另外,没有导管,而将分泌物放到血管中的分泌细胞群,也同样是腺...淋巴细胞在对抗原和促细胞分裂剂(mitogen)进行反应而增殖分化中,

lymphoma：淋巴瘤

淋巴瘤(lymphoma)是起源于淋巴结和淋巴组织的免疫系统恶性肿瘤,其发生大多与免疫应答过程中淋巴细胞增殖分化产生的某种免疫细胞恶变有关,以无痛性进行性的淋巴结肿大和局部肿块为其特征性的临床表现,并可有相应器官压迫症状.

lymphopoiesis：淋巴细胞增殖

lymphopenia 淋巴细胞减少 | lymphopoiesis 淋巴细胞增殖 | lymphopoietic 淋巴细胞增殖的

phosphonium：磷

Kegley等(1996)研究发现,随日粮磷(Phosphonium)水平增加,断奶仔猪SRBC(致敏红细胞)抗体和卵清蛋白抗体水平都呈线性下降;PHA刺激的淋巴细胞增殖反应显著增强;皮下注射PHA后,皮褶厚度(超敏反应)呈曲线增强,表明提高日粮磷水平,

clonal selection：克隆选择

为解决这个问题,接触抗原后被刺激的淋巴细胞进行增殖(图2.6b),形成一个大的浆细胞克隆,其产生的抗体与最初淋巴细胞的抗体一致. 通过这个克隆选择(clonal selection)的系统可以产生足够的抗体,有效地对抗感染(要事2.1、图M2.1.1 ).

LAK：自然杀伤细胞

1.本品具有广谱抗病毒作用和明显的免疫增强功能,可快速恢复机体外周T淋巴细胞的增殖,激活自然杀伤细胞(LAK)生理活性,分泌抗体,诱生干扰素(IFN)干扰病毒DNA和DHA的合成;对病毒具有直接灭杀、阻挡侵染和抑制病毒繁殖作用.

lymphopoietic：淋巴细胞增殖的

lymphopoiesis 淋巴细胞增殖 | lymphopoietic 淋巴细胞增殖的 | lymphosarcoma 淋巴肉瘤

hybridoma cell：杂交瘤细胞

单克隆抗体技术的核心是用骨髓瘤细胞(myeloma cell)与经特定抗源免疫刺激的B淋巴细胞(antigen stimulated B lymphoblast)融合得到杂交瘤细胞(hybridoma cell),杂交瘤细胞既能像骨髓瘤细胞那样在体外无限增殖,又具有B淋巴细胞产生特异性抗体的能力.

mitogen：促细胞分裂剂

A细胞 (A cell)淋巴细胞在对抗原和促细胞分裂剂(mitogen)进行反应而增殖分化中,在大多数的情况下,并不单纯出于淋巴细胞的反应,而在细胞膜表面出现的Ia非淋巴细胞与之共存是必要的,这些细胞统称为A细胞或副卫细胞(accessory cell).

APB：成人外周血

(CB)、成人外周血(APB)的单个核细胞(MNC)和CD56+淋巴细胞亚群,发现IL-15对CB和APB的MNC的扩增效应优于IL-12,且IL-12联合IL-15促进3种细胞株的增殖效果强于两者单用这些发现为CB移植的患者进行细胞因子治疗提供了理论依据.