消化液培养基

词组短语

digest medium

与消化液培养基相关的网络例句 [注：此内容来源于网络，仅供参考]

The plasmid of pEGFP-N1 was preserved in our laboratory.②The rat vibrissa follicles were dissected under a stereomicroscope. The dermal sheath was moved by incubated in dispase. The bulge regions of the hair follicles in anagen phase were carefully cut between the arrector pili muscle and the sebaceous gland, and then incubated with a mixture of trypsin and EDTA. The cell suspension was selected, and cultured in 10% fetal bovine serum DMEM/F12 FAD medium. After 7 days culture, HFSC was obtained by rapid adhering on collagen Ⅳ.

实验方法：大鼠在体视显微镜下分离出含真皮鞘的完整毛囊，dispase消化，将毛囊从真皮鞘中挤出，收集形态完好且处于生长期的毛囊，分别在毛球部上端、皮脂腺下端横切毛囊，取中间部分置于胰酶和EDTA中联合消化，向所得细胞悬液中添加含10%胎牛血清DMEM/F12完全FAD培养基，常规培养7 d后，采用IV型胶原快速贴壁法两次筛选以分离纯化大鼠毛囊干细胞。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence ＋ serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁＋无血清条件培养液纯化的实验。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

This can be done by adding 1 mL of not less than 10 3 dilution of a 24-hour broth culture of the microorganism to the first dilution (in pH 7.2 Phosphate Buffer, Fluid Soybean–Casein Digest Medium, or Fluid Lactose Medium) of the test material and following the test procedure.

方法如下：将1mL该微生物24小时肉汤培养物的10 3倍稀释液加入供试品的首次稀释液（于pH7.2磷酸盐缓冲液，液体大豆酪蛋白消化物培养基，或液体乳糖培养基中）。

With either method, first dissolve or suspend 10.0 g of the specimen if it is a solid, or 10 mL, accurately measured, if the specimen is a liquid, in pH 7.2 Phosphate Buffer, Fluid Soybean–Casein Digest Medium, or Fluid Casein Digest–Soy Lecithin-Polysorbate 20 Medium to make 100 mL.

不管使用哪一种方法，首先将10.0g固体样品或精密量取的10mL液体样品，放入pH 7.2的磷酸盐缓冲液，或液体大豆酪蛋白消化物培养基，或液体酪蛋白消化物-大豆卵磷脂-聚山梨酯20中，制成100mL的溶液或悬浮液。