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Results The amaranthine nuclei had a distinct outline and distributed evenly on the slide. The area of nuclei in liquidbased preparation was much smaller and chromatin was more condensed in comparison with those in conventional smear. The nuclei in conventional smears had the largest value of area and the value of AOD, and CV of IOD, the IOD ratio among diploid, tetraploid and octaploid DNA was most close to 2 and 4, and the CV of IOD exceeded 6%.
结果 三种涂片内肝细胞核分布均匀,轮廓清晰,呈紫红色,与传统涂片法相比,液基法内肝细胞核面积明显减小,染色质高度浓缩;相同倍体的肝细胞核在传统涂片中面积最大,平均光密度最低,各倍体间的比值最接近2和4,积分光密度CV值最低(。5)。
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In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.
结果表明,嗜热链球菌在营养丰富的培养基中的生长延滞期缩短,到达对数生长顶点的时间变短,稳定期的时间延长,当经过二次培养后,嗜热链球菌几乎不经过延滞期直接到达对数期,平稳期持续的时间较短,很快到达衰亡期;嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系,菌数多,其代谢旺盛,菌数降低其代谢速度减慢;嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化,并可能存在着细胞凋亡,与菌体增殖和产酸规律密切相关,随着培养时间延长,培养基中营养物质变得贫乏,菌体形态变化和细胞凋亡的情况愈加明显。
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The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.
结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。
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Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.
结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。
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Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.
结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。
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N in vivo study, Application of wildtype N-Ihh to the interdigital spaces in chick embryos resulted in up-regulated activity of Ihh signal pathway in mesenchyme and elongation of penultimate phalange, even an appearance of extra phalange.
hh在鸡胚中的表达模式分析表明在指顶端的浓缩体中表达的Ihh可能调控了指的形成。
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The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.
结果:(1)通过细胞培养和MTT法,表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用,加药组与对照组相比其生长抑制率有显著性差异(P<0.01),而且这种抑制作用呈现浓度和时间的依赖性;(2)通过集落形成率的测定结果表明,加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P<0.01),说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用;(3)光镜观察结果表明,加药组可见细胞脱水浓缩伊红染色增强,胞体缩小,内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态;经AO/EB荧光染色观察结果表明,当终浓度为1.0mg/ml的姬松茸提取物作用于MGC-803胃癌细胞24h后,其凋亡率和破膜率与自然凋亡率与破膜率相比,均有显著性差异,其凋亡率86.3%(P<0.001),破膜率为41.6%(P<0.01),说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用,同时也有细胞毒作用,但以诱导细胞凋亡为主;(4)免疫组化结果表明,用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P<0.001)。
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The former formed a kind of KR cyst, while the later formed a kind of NKR cyst. All the kinetosomes, ciliary shafts and microtubules were resorbed in the former, meanwhile, the cell lost most water and cell volume decreased distinctly. In the later, only part of kinetosomes and part of microtubules were resorbed. Cell lost water slightly and volume decreased slightly.
期间,细胞吸收了全部的毛基体、纤毛杆和微管结构,伴随着细胞剧烈的失水浓缩,细胞体积显著缩小;游仆虫在形成毛基体非吸收型包囊时,细胞仅经历了部分的脱分化过程,期间,细胞仅吸收了部分的毛基体及部分微管结构,并且细胞仅发生了轻微的脱水和浓缩,细胞以折叠的形式容纳在包囊壁内。
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In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.
在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后,先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时,此时卵母细胞已进入第一次减数分裂的前中期,并且在DIC倒置显微镜下可以看到浓缩的染色体,用Polscope可以发现明显的纺锤体,借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换,经过三次直流电脉冲作用后,分别有91.6%的胞质—MI核质体对融合,经过进一步的培养后,超过80%的重组卵母细胞排出第一极体,体外受精后分别有79%和77%的重组卵形成双原核,受精后的胚胎在KSOM胚胎培养液中体外培养4天后,超过60%的胚胎发育至囊胚。
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Brent and shutter of pulverized coal concentrators was investigated and tested.
对两种煤粉浓缩器丘体和百叶窗的浓缩能力进行了试验研究。
- 更多网络解释与浓缩体相关的网络解释 [注:此内容来源于网络,仅供参考]
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acrosome:顶体
精子形成的主要变化是:①细胞核染色质极度浓缩,核变长并移向细胞的一侧,构成精子的头部;②高尔基复合体形成顶体泡,逐渐增大,凹陷为双层帽状覆盖在核的头端,成为顶体(acrosome);③中心粒迁移到细胞核的尾侧(顶体的相对侧),发出轴丝,
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arthrospore:节孢子
3.节孢子(arthrospore)菌丝生长到一定阶段,长出许多横隔,然后从横隔处断裂,产生许多单个孢子,即节孢子,又称粉孢子. 4.厚垣孢子(chlamydospore)在菌丝顶端或中间,一部分原生质浓缩、变圆,细胞壁加厚,形成厚垣孢子,又称厚壁孢子,是繁殖体,
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Inclusion bodies:包涵体
为此,本文在原核细胞里表达nectin-2 V结构域的包涵体(inclusion bodies),经体外复性和纯化后得到可溶性蛋白,将其浓缩至浓度为5mg/ml和10mg/ml,使用悬滴气相扩散法生长晶体,并对晶体生长的条件进行优化.
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Hyla chinensis:雨 蛙
研究了中国雨蛙(Hyla chinensis)精子的超微结构,并初步探讨其在系统发育上的意义. 中国雨蛙精子由头部和尾部两部分组成. 头部有一棒状的细胞核,核内染色质高度浓缩. 细胞核前方有顶体. 顶体圆锥状. 顶体下腔之中有一圆锥状的顶体下锥和细小的囊泡.
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Hyla chinensis:中国雨蛙
摘 要 研究了中国雨蛙(Hyla chinensis)精子的超微结构,并初步探讨其在系统发育上的意义. 中国雨蛙精子由头部和尾部两部分组成. 头部有一棒状的细胞核,核内染色质高度浓缩. 细胞核前方有顶体. 顶体圆锥状. 顶体下腔之中有一圆锥状的顶体下锥和细小的囊泡.
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Pilus:菌毛
(三)菌毛(pilus) 许多革兰阴性菌和少数革兰阳性菌菌体上附着的比鞭毛更细、短而直,数目较多的丝状物,称为菌毛. 普通光镜下不可见. (四)芽胞(spore) 某些革兰阳性菌在一定环境条件下,胞浆脱水浓缩,在菌体内部形成一个圆形或卵圆形的小体,
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hemoconcentration:血浓缩 血液浓缩
hemocoel 昆虫血腔 | hemoconcentration 血浓缩 血液浓缩 | hemoconia 血尘 血尘 苗勒氏尘状体
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hemoconia:血尘 血尘 苗勒氏尘状体
hemoconcentration 血浓缩 血液浓缩 | hemoconia 血尘 血尘 苗勒氏尘状体 | hemoconiosis 血尘病
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mitotic spindle:有丝分裂纺锤体
1)标志前期开始的第一个特征是染色质开始浓缩(condensation)形成有丝分裂染色体(mitotic chromosome)2)第二个特征细胞骨架解聚,有丝分裂纺锤体(mitotic spindle)开始装配4)染色体由两条染色单体(chromatid)构成1)所有染色体排列到赤道板(Metaphase Plate)上,
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luteinizing hormone:促黄体素
Preti在费城的研究小组发现,当他们把男性腋下的分泌物浓缩至棉垫后,让志愿接受的女性实验者携带长达六小时,接着再观察他们体内促黄体素(luteinizing hormone)的变化.