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Ointment extracted from Plastrum Testudinis was separated by silica gel column chromatography, gradiently eluted by petroleum ether-ethyl acetate, and 16 (Ts-1~Ts-16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells. Results showed that Ts-12 induced proliferation compared with the control group ( p <0.05), while Ts-4 showed inhibitive effect. Other groups showed no significance ( p >0.05). GC-MS was used to analyze the chemical components in different groups.
中药龟板提取物浸膏经硅胶柱层析,用石油醚-乙酸乙酯作洗脱剂,梯度洗脱,得到16个组分;采用MTT法和流式细胞技术研究了它们对鼠骨髓间充质干细胞的增殖作用,实验结果显示组分Ts-12具有促进rMSCs增殖的作用( p <0.05),而组分Ts-4则表现出抑制rMSCs增殖的作用( p <0.05),其它样品对rMSCs的增殖作用不显著( p >0.05),不具统计学意义。
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objectiveto predict the promoting effect of chinese medicine on proliferation of rat bone marrow mesenchymal stem cells by bioactivity fingerprint in vitro.methodsthe ointment of plastrum testudinis was extracted by petroleum ether,ether and dichloromethane in burn to gain the extracts.the effects on the proliferation of bmmscs were examined by mtt assay and flow cytometry analysis, and each sample was studied by gc-ms.
目的运用生物活性指纹图谱预测其它中药能否促进骨髓间充质干细胞(bone marrow mesenchymal stem cells,bmmscs)。方法龟版浸膏依次用石油醚、乙醚、二氯甲烷提取,得到溶剂提取物,用mtt 比色法及流式细胞仪器研究了溶剂提取物干细胞活性,采用gc-ms 技术研究每一个提取物,根据总离子流色谱图的相同保留时间和相应峰面积,获得龟版浸膏 gc-ms 生物活性指纹图谱。
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Methods: the SMMC7721 cancer cell line were cultured in the absence of differente concentration Daphne odora Var.Marginata Jingao and assary the cell cycle by flow cytometry . MTT method was used to examine the effects of different concentration Daphne odora Var.
采用流式细胞术,观察不同浓度金边瑞香浸膏对SMMC7721细胞增殖周期的影响;采用MTT法检测不同浓度金边瑞香浸膏对SMMC7721, NKM45, A549, S180肿瘤细胞株增殖作用的影响。
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ConclusionThe extracts of Phrynium capitatum Willd flower with butyl alcohol and with water have excitatory effects on the non-pregnant or diethylstilbestrol-injected animal uterus in vitro ,which is more potent than that of Herba Leonuri fluid extract.
结论棕叶花正丁醇、水溶性组分对家兔、豚鼠、大鼠、小鼠离体子宫有明显兴奋作用。对离体子宫兴奋作用比益母草流浸膏显著。
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MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract.
目的探讨棕叶花不同提取物对家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用。方法采用BL-410生物机能实验系统,观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用,并用EC50与益母草流浸膏进行比较。
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ConclusionThe extracts of Phrynium capitatum Willd flower with butyl alcohol and with water have excitatory effects on the non-pregnant or diethylstilbestrol-injected animal uterus in vitro ,which is more potent than that of Herba Leonuri fluid extract.
结论棕叶花正丁醇、水溶性组分对家兔、豚鼠、大鼠、小鼠离体子宫有明显兴奋作用。对离体子宫兴奋作用比益母*流浸膏显著。
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MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract.
方法采用BL-410生物机能实验系统,观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用,并用EC50与益母草流浸膏进行比较。
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Results:The prescription of Schisandra liquid extracthave the distinguishing characteristics of the thin-layer chromatography.
结果:处方中的五味子流浸膏(五味子乙素、五味子甲素)的薄层色谱具有鉴别特征。
- 更多网络解释与流浸膏相关的网络解释 [注:此内容来源于网络,仅供参考]
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belladonna root:颠茄根
belladonna liquid extract 颠茄流浸膏 | belladonna root 颠茄根 | belladonna 颠茄
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liquid face powder:增白粉蜜
liquid extract 流浸膏 | liquid face powder 增白粉蜜 | liquid fertilizer injection system 液体肥料喷射系统
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liquid face powder:增****蜜
liquid extract 流浸膏 | liquid face powder 增****蜜 | liquid fertilizer planter 液肥播种机
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fluidextract:流浸膏 流浸膏剂 液体栲胶
fluiderosion 流体侵蚀 | fluidextract 流浸膏 流浸膏剂 液体栲胶 | fluidexudation 液体渗出
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fluidextract:流浸膏
floccules絮凝物、絮凝块 | Fluidextract流浸膏 | flux流量、通量
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Ipecac Extract:吐根流浸膏
扑尔敏 Chlorphenamine Meleate | 吐根流浸膏 Ipecac Extract | 利血平 Reserpine
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liver fluid extract:肝流浸膏
liver flap 扑翼样震颤 | liver fluid extract 肝流浸膏 | liver function 肝功能,肝机能
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malt liquor:麦芽浆
malt liquid extract 麦芽流浸膏 | malt liquor 麦芽浆 | malt meal 麦芽粉
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ergotaminine:麦角胺宁 麦角异胺
ergotaminetartrateinjection 酒石酸麦角胺注射液 酒石酸麦角胺注射液 | ergotaminine 麦角胺宁 麦角异胺 | ergotfluidextract 麦角流浸膏
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Licorice Root Extract:甘草流浸膏
103.006 风信子浸膏Hyacinth concrete | 103.007 甘草流浸膏Licorice root extract | 103.008 桂花浸膏Osmanthus concrete