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P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.
丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。
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It provides a route tothe compounds which contains three functional groups, two carbon-nitrogentriple bonds and one carbon-carbon double bond. In water, metallic indiumpromotes carbon-sulfur bond coupling reaction of aromatic sulfonyl chloride withsuitable organic halides to afford sulfones. In the last chapter, metallic tin mediated allylation of aldimines with allylbromide was revealed.
第三章，我们研究了在水相介质中金属铟促进的烯丙基溴对1，1-二氰基-2-芳基乙烯的Michael加成反应，生成一个含碳碳双键和二个碳氮叁键的三官能团化合物，另外，金属铟在水介质中还可以促进芳基磺酰氯与卤代烃发生碳硫键的偶联，生成砜。
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RCM reactions of 49 and 50 are chosen to evaluate the catalytic performance ofthe complexes 46-48 because they are informative and simple to manipulate. The resultsindicated that ruthenium benzylidene complexes 46 and 48 possess significant thermalstability and cyano group tolerance.
由于烯烃49、50的闭环交互置换反应简单、清晰，故用来考察新钌卡宾配合物的催化性能，结果表明配合物46、48具有良好的热稳定性及耐氰基性能。
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Based on studies of RCM reaction of 50 and CM reaction ofacrylonitrile with 1-octene or 1-decene catalyzed by pyridine derivative-coordinatedruthenium benzylidene complexes, it can be concluded that ruthenium benzylidene complexwith the more labile dissociating ligand exhibits the higher initial activity and better catalyticperformance.
吡啶衍生物配位的钌卡宾配合物催化含氰基烯烃50闭环交互置换反应和丙烯腈与1-辛烯、1-癸烯交叉交互置换反应表明，催化剂中吡啶衍生物配体越易解离，其初活性越高，催化活性越好。
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In this catalyst system, the Knoevenagel condensations of a series of aromatic aldehydes with malononitrile or ethyl cyanoacetate were programmed and a mechanism was postulated as well. When aromatic aldehydes reacted with ethyl cyanoacetate, the corresponding alkenes proved to be E-isomers by 1H NMR.
在该反应体系中考察了一系列芳香醛和丙二氰、氰基乙酸乙酯的缩合反应，并推测了Knoevenagel缩合反应的机理，芳香醛与氰基乙酸乙酯缩合产物经1H NMR检测为E式烯烃。
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Chemical elements and their compounds, excluding the hydrocarbons and their derivatives (except carbides, carbonates, cyanides, cyanates and carbon disulfide).
不含除碳化物，碳酸盐，氰化物，氰酸盐及二硫化碳外的烃基及烃基衍生物的化学元素及其化合物。
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These substituents are either small groups such as phenoxy groups,aryls,cyclic secondary amines,cyano groups,and halogens or large functional moieties such as PDI,Phthalocyanines, porphyrins,BODIPY,cyclodextrin,Fullerene,calixarene,and so on.
这在过去的工作中，人们把一些小分子的，如酚氧基、仲胺基、苯基、氰基以及卤素氟、氯、引入到PDI中，同时也把一些较大功能团，如PDI本身、卟啉、酞菁、环糊精、BODIPY、杯芳烃、富勒烯等和PDI连接在一起。
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dicyclic hydrocarbon：双环烃
dicyandiamide 双氰胺 | dicyanobutylene 二氰基丁烯 | dicyclic hydrocarbon 双环烃
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cyanocarbon：氰基烃
cyanite /蓝晶石/ | cyanocarbon /氰基烃/ | cyanogen /氰/
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polyesters：多元酯
并应符合下列要求:为聚异氰酸盐(Polyisocyanate)与聚合醚聚合烯烃(Polyether Polyols)或其他如聚醚(Polyethers)、多元酯(Polyesters)、两剂混合并加三碳氢基氨(Tertiary Amines)及锡盐(Tin Salts)等以控制反应速率及胶结与发泡速率