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A ratio spectra derivative spectrophotometry for simultaneous determination of quaternary mixture of aminopyrine, phenobarbital, caffeine and phenacetin was studied.
提出了可应用于四元混合体系同时测定的比光谱-导数分光光度法,阐述了该方法的基本原理,并将其应用于氨基比林、苯巴比妥、咖啡因和非那西丁四元混合体系中各组分的同时测定。
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For example: antimalarials primaquine morpholine, flutter malaria quinoline, quinine, chloroquine, quinacrine; antibacterial drugs have sulfa drugs, chloramphenicol and furans; antipyretic analgesics are acetyl salicylic acid, phenacetin, acetanilide, antipyrine, aminopyrine; other drugs such as vitamin K, probenecid, hydralazine, quinidine, methylene blue, toluene and so on.
抗疟药伯氨喹啉、扑疟喹啉、奎宁、氯奎、阿的平;抗菌药有磺胺类药物、氯霉素及呋喃类;解热镇痛药有乙酰水杨酸、非那西丁、乙酰苯胺、安替比林、氨基比林;其它药物如维生素K、丙磺舒、肼苯哒嗪、奎尼丁、美蓝、甲苯等。
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Results: The retention time of phenacetin, paracetamol and IS were 4.1, 1.4 and 2.8 min, respectively.
结果:非那西丁、对乙酰氨基酚和内标的保留时间分别为4.1、1.4和2.8 min。
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Sulfamonomethoxine was used as internal standard. Phenacetin, paracetamol and IS were monitored by multiple reaction monitoring mode with ion transitions of m/z 180→138, 152→110 and 281→156, respectively.
以磺胺甲氧嘧啶为内标,非那西丁、对乙酰氨基酚和内标的多反应监测离子对分别为m/z180→138、152→110和281→156。
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Results Four components including caffine, aminophyrine, phenacetin and phenobarbital were completely separated and determined.
结果 样品中咖啡因、氨基比林、非那西丁、苯巴比妥等4组分完全分离且呈良好线性关系,其加样回收率为98.26%~101.87%。
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The accuracy of phenacetin and paracetamol was 87.1%-101.5% and 89.5%-107.3%, the precision was 0.85%-7.83% and 2.53%-8.74%, respectively. The extraction recovery ranged from 58.25% to 69.85%, and matrix effect was 99.0% to 103.6%.
非那西丁和对乙酰氨基酚的准确度分别为87.1%~101.5%和89.5%~107.3%,精密度分别为0.85%~7.83%和2.53%~8.74%,提取回收率为53.0%~76.0%,基质效应为92.0%~106.3%,均符合生物样本分析方法的有关要求。
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Methods Paracetamol was analyzed with HPLC,ODSC18 was packed in the analytical column,and phenacetin as internal standard.the mobile phase consisted of methanolwater(70:30)and the flow rate was 1.0 ml/min,The UV detection wave length was 257 nm.
采用ODSC 18色谱柱,内标为非那西丁,流动相为甲醇水(70:30,v/v),流速1.0 ml/min,检测波长257 nm,测定泰诺片中对乙酰氨基酚的含量。
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The mean recovery of phenacetin,caffeine,phenobabital and aminophenazone were 100.4%,100.3%,99.5%,and 100.4% respectively.
非那西丁、咖啡因、苯巴比妥、氨基比林的平均回收率分别为100.4%,100.3%,99.5%和100.4%,RSD分别为0.90%,1.9%,1.8%,1.6%(n=6)。
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The experiential mathematical model was built up by using stepwise regression and optimized with modifiedsimplex method, then five components- amidopyrine ,phenacetine,theophylline, theobromine and caffeine in compound theophyllinitablets were determined simultaneously.
利用逐步回归分析法建立经验数学模型,再运用改良单纯形法进行优化,同时测定复方茶碱片中的五种组分——氨基比林、非那西丁、茶碱、可可碱和咖啡因的含量,其回收率分别为99.5%、100.2%、101.2%、100.2%和99.2%,RSD分别为1.51%、1.03%、4.44%、2.47%和3.12%。