次培养

基本解释 (translations)

subculture

与次培养相关的网络例句 [注：此内容来源于网络，仅供参考]

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明，嗜热链球菌在营养丰富的培养基中的生长延滞期缩短，到达对数生长顶点的时间变短，稳定期的时间延长，当经过二次培养后，嗜热链球菌几乎不经过延滞期直接到达对数期，平稳期持续的时间较短，很快到达衰亡期；嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系，菌数多，其代谢旺盛，菌数降低其代谢速度减慢；嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化，并可能存在着细胞凋亡，与菌体增殖和产酸规律密切相关，随着培养时间延长，培养基中营养物质变得贫乏，菌体形态变化和细胞凋亡的情况愈加明显。

This is the first time I cultivated Narcissus and is only thing placed to celebrate the Spring Festival at home.

这是我第一次培养水仙花﹐也是春节唯一的应节品。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

With involv ement of essential elements of ind ividual moral q ualities such as virtu es, to lerance and

在此基础上，结合中国当前中小学教育的状况，可以通过以下三种方式，分层次培养

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Results a conception of nursing researcher cultivation according to level and its specific methods were suggested, which could lay a solid foundation for the development of nursing research in sanitariums.

结果 提出了分层次培养护理科研人才的构想和具体落实方法，从而为疗养院护理科研的发展奠定坚实基础。

Subsequently, the performance of the Pastorex Staph-Plus agglutination test with presumptive MRSA isolates taken directly from chromogenic agars was compared to that of the Pastorex Staph-Plus agglutination test with isolates from blood agar subcultures (conventional_Pastorex agglutination).

紧接着，使用Pastorex Staph-Plus葡萄球凝集试剂检测直接从显色平板上挑取的疑似MRSA菌落（称之为直接Pastorex 凝集），并与次培养到血平板上的菌落的Pastorex Staph-Plus凝集试验（称之为传统Pastorex 凝集）的结果进行比较。

Results In 136 patients, a total of 72cases sputum culture positive, the positive rate of 52.9% sputum culture for three consecutive single bacteria were 16 cases, the positive rate of 11.8%.16 patients sputum results were:hemolytic streptococcus 6 cases, 4cases of Pseudomonas aeruginosa , Klebsiella pneumoniae in 2 cases and gas production Bacillus 2 cases , Enterobacter Bacillus 2 cases, 136 patients with 386 cases sputum culture times , 106 times positive(27.5%), and consecutive three or more times a train 4 the same bacteria ,only 42 cases(10.8%); the same single-64 cases sputum culture , which amounts to 16.6%.

结果 在136例患者，共72例痰培养阳性，阳性率52.9%，痰培养连续3次为单一细菌者为16例，阳性率为11.8%。16例患者痰菌结果分别为：溶血性链球菌6例，绿脓杆菌4例，克雷伯氏杆菌2例，产气杆菌2例，阴沟杆菌2例，136例患者386例次痰菌培养，106例次阳性（27.5%），而连续3次以上培养为相同一细菌，仅为42例次（10.8%）；非相同单一痰菌培养64例次，占16.6%。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

The next frontier for Nike is to move even deeper into the subcultures by making the actual equipment

Nike的下一步计划是通过制造实际的设备将触角伸入到次培养中。

与次培养相关的网络解释 [注：此内容来源于网络，仅供参考]

AFB：抗酸杆菌

(2)细菌原则学标准:有下列情况之一者特色可以确诊:A.1年内至少有3次痰/支气管冲洗标本,3次培养阳性,抗酸杆菌(AFB)涂片阴性;或2次培养阳性,1次AFB涂片阳性.

primary crystallization：一次结晶

primary crushing 初次破碎 | primary crystallization 一次结晶 | primary culture 初级培养物,原始培养,原代培养

duckweed：浮萍

当鸭子从盖满 浮萍(duckweed)的池塘突然走出时,我曾两次看到这些小植物附着在它们的背上;并 且曾经发生过这样的事情:把一些浮萍从一个水族培养器移到另一个水族培养器里时, 我曾无意中把一个水族培养器里的贝类移入到另一个.

feeding：饲养

24h后倾去培养皿内种植培养液,改用饲养(Feeding)培养液培养. 接种第3d, 在培养皿中分别加入细胞分裂抑制剂5-氟-2'-脱氧尿苷15μg/ml和尿苷35μg/ml, 作用48 h后更换新鲜饲养培养液,以后每周换液两次, 每次更换一半新鲜饲养培养液.

GIT：生长抑制试验

长出的菌落没有明显周边. 多次传代后生长加快,菌落成典型荷包蛋样. 分离的支原体可经形态、血细胞吸附、生化反应以及特异性抗血清作生长抑制试验(GIT)进行鉴定. 培养阳性率差,血清学诊断阳性者培养仅64%阳性.

Penicillium citrinum：橘青霉

橘青霉(Penicillium citrinum)经培养,深层发酵、提取、多次过滤和纯化精制而成. 蜂蜜曲霉(Aspergillus melleus)经培养,深层发酵、提取、多次过滤和纯化精制而成. 蜂蜜曲霉(Aspergillus melleus)经培养,深层发酵、提取、多次过滤和纯化精制而成.

secondary cell culture：次代细胞培养

secondary cell 二次电池=>二次電池,蓄電池 | secondary cell culture 次代细胞培养 | secondary cell wall 次生细胞壁

secondary culture cell：次生培养细胞

secondary culture 次级培养 | secondary culture cell 次生培养细胞 | secondary effect 二次酌

subculture：传代培养

从体内取出组织接种培养到第一次传代,随不同的组织时间长短不一,一般为1~4w 2.传代培养(Subculture)阶段:或称继代培养. 也就是细胞系(Cell line)阶段,细胞增殖旺盛,一般细胞可传代10-50代).

mycobacteria：分枝杆菌

但培养14天和进行肉汤末次接种会更有效. 当临床症状提示为弗朗西丝菌(Francisella)感染时,阳性血液培养物需要接种到BCYE琼脂培养基,所有操作应在生物安全三级的层流罩超净台中进行. 3、 分枝杆菌(Mycobacteria) 使用常规血培养基不能分离.