- 更多网络例句与核酸组蛋白相关的网络例句 [注：此内容来源于网络，仅供参考]
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Methods:Immunohistochemistry and nonradioactive in situ hybridization methods were used to detect the expression of p27mRNA and protein in 58 cases of osteoplastic tumors.
采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中p27蛋白和mRNA的表达状况。
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Japonicum, covering 90% of its total genes; find 8400 protein-coding genes; set up the largest functional gene database and clone libraries in the world; for the first time, identify as many as 3260 proteins from variety of life stages of S. japonicum; analyse the eggshell proteins and tegument proteins, which were the putative targets for drugs and candidates of vaccines; reveal a large quantity of genetic polymorphisms in the genes of S.
对人CD34+造血干／祖细胞进行了转录组和蛋白质组学研究，鉴定了370个蛋白质，这是迄今为止鉴定最多的CD34+细胞蛋白，并且鉴定了各种转录组研究没能发现的133个CD34+细胞蛋白，发现了CD34+细胞具有可塑性的新的证据，证明了反义核酸未能完全阻止其相应基因的表达，并提出一些蛋白的异质性是由于翻译后修饰造成；2。
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The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.
应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。
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Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.
采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型，同时以何首乌饮灌胃作为延缓衰老组；造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况；测定各组Fas和FasL的信使核糖核酸水平；SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。
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The Center for Advanced Biomolecular Research is primarily dedicated to education and research in biomolecular including mechanism and regulation of enzymes, Protein folding and stability, proteomics, enzyme regulation and design, mechanisms of enzyme allostery, nuclear magnetic resonance, structural biology, proteins and RNA folding thermodynamics.
高级生物分子研究中心主要致力于生物分子的教学与研究，其研究领域包括酶作用机理，蛋白折叠和稳定性，蛋白组学，酶调控和设计，酶变构机制，核磁共振，结构生物学，蛋白质和核糖核酸折叠热力学等。
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Any of a group of RNA-containing viruses, including those that cause influenza, typically having an affinity for certain mucins and causing agglutination of red blood cells.
粘液病毒一组含核醣核酸病毒的任一种，包括引起流行性感冒的病毒，通常与某种粘蛋白有关，而且会导致血红细胞的凝集
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The proteins that combine with DNA are generally of characteristic types called histones and protamines.
与去氧核糖核酸结合者通常属于被称为组蛋白或鱼精蛋白的特殊种类。
- 更多网络解释与核酸组蛋白相关的网络解释 [注：此内容来源于网络，仅供参考]
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thymus histone：胸腺组织蛋白;胸腺组朊
胸腺 thymus gland | 胸腺组织蛋白;胸腺组朊 thymus histone | 胸腺核酸 thymus nucleic acid; thymonucleic acid
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nuclease：核酸酶
端粒酶与真核生物线形DNA末端的复制有关,它有一种--ARNA聚合酶( RNA polymerase)B逆转录酶(reverse transcriptase)C核酸酶(nuclease)D 核糖核酸酶(ribonuclease rnase)13.真核生物的组蛋白上的修饰作用,除了乙酰基化、甲基化和磷酸化外,
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micrococcal nuclease：微球菌核酸酶
RKO细胞转染cyclin G基因后,对微球菌核酸酶(micrococcal nuclease)敏感性增强,与亲本细胞相比,转染细胞染色质结构去凝集. 组蛋白H1的磷酸化是cyclin g转染细胞染色质去凝集的焦虑可能机理. 从cyclin G过表达细胞分离的cdk2免疫复合物在体外组蛋白H1的磷酸化能力增强,
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nucleohistone：核酸组蛋白
nucleocapsid [病毒]核壳(体),核衣壳 | nucleohistone 核酸组蛋白 | nucleolar 核仁的
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pyridoxine：吡哆醇
维生素B6包括六种可以互相变换[de]吡哆醇(pyridoxine)、吡哆胺(pyridoxamine)、吡哆醛(pyridoxal)和它们各自[de]5-磷酸化物. 吡哆醛5-磷酸盐是这一组中[de]重要部分,在脱羧作用及氨基转移作用中作为酶系统[de]辅酶参与体内氨基酸、蛋白、脂类、核酸及糖原[de]代谢.
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nucleolar：核仁的
nucleohistone 核酸组蛋白 | nucleolar 核仁的 | nucleoli (复数)核仁
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thymus nucleic acid; thymonucleic acid：胸腺核酸
胸腺组织蛋白;胸腺组朊 thymus histone | 胸腺核酸 thymus nucleic acid; thymonucleic acid | 甲杓肌;盾瓢肌 thyreoarytaenoideus muscle