- 更多网络例句与核酶相关的网络例句 [注：此内容来源于网络，仅供参考]
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Computer was used to analyze the possible secondary cleavage sites on HPV11 E2 mRNA and to predict the secondary structures of substrate and ribozymes. According to the theory of Symons headhammer ribozyme, there are 32 sites to targetting sequences.
遵循Symons锤头状核酶结构和GUX剪切位点原则，靶序列存在32个剪切位点，通过计算机软件分析核酶的最佳剪切位点，并对底物及核酶的二级结构进行预测及进行相应基因生物学功能和基因同源性分析，筛选出2个锤头结构核酶。
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Furthermore, this novel approach would have potential in the research on human disease detection and diagnosis synchronously.
为核酶活性分析、核酶动力学研究提供新型有效的手段，也为核酶基因药物的深入研究提供了新的方法和思路。
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Methods: Five 10-23 deoxyribozymes (DZ1-DZ5) with 2 phosphorothioate groups at 5' and 3' end were designed and synthesized. Full sequence ET-1 RNA was transcripted and FAM-labeled 10-23 deoxyribozyme was used to select cleavable 10-23 deoxyribozyme in vitro and to detect intracellular uptake. The content of ET-1 mRNA was measured by semi-quantitative RT-PCR after 10-23 deoxyribozyme was transfected into cultured neonatal rat cardiomyocytes.
体外转录ET-1全长RNA底物，设计并合成5条ET-1 10-23脱氧核酶(DZ1~DZ5)，其5'及3'端各有2个核苷酸硫代修饰，体外切割ET-1RNA底物，筛选有效脱氧核酶；5'标记荧光素FAM的10-23脱氧核酶瞬间转染新生大鼠心肌细胞以观察对10-23脱氧核酶的摄取；采用半定量RT-PCR检测ET-1基因的表达。
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Ribozyme can inhibit the expression of HBeAg in the cotransfected cells by cleaving the mRNA of HBV.
在核酶与HBV共转染细胞中，核酶通过对HBV mRNA的剪切作用，使HBeAg的表达量明显受到抑制。
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The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.
分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。
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We construct the eukaryon expression vector containing the tRNA-CTE hybrid ribozyme gene successfully which is the foundation for further study.
结论tRNA-CTE复合核酶真核表达载体的成功构建，为开展利用tRNA-CTE复合核酶切割HCV RNA的研究奠定了基础。
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Using in situ hybridization, we found that ribozyme transcripts were predominantly located in the nucleus of transgenic potatoes, consistent with the hypothesis that ribozymes were more efficient against nuclearly replicative viroid.
原位杂交结果表明核酶转录产物主要分布在转基因马铃薯细胞的细胞核中。这一结果支持我们提出的核酶对在核内复制的类病毒更为有效的假说。
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The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.
根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育 2h。
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ABSTRACT］ObjectiveTo construct a eukaryotic expression vector of M1RNA ribozyme against telomerase RNA and transfect to hepatocellular carcinoma cell lines.
摘要］ 目的构建抗人端粒酶RNA的M1RNA核酶的真核表达载体，并筛选稳定转染核酶的肝癌细胞株。
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Objective To construct a eukaryotic expression vector of M1RNA ribozyme against telomerase RNA and transfect to hepatocellular carcinoma cell liness.
目的 构建抗人端粒酶RNA的M1RNA核酶的真核表达载体，并筛选稳定转染核酶的肝癌细胞株。
- 更多网络解释与核酶相关的网络解释 [注：此内容来源于网络，仅供参考]
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hammerhead ribozyme：锤头状核酶
抗HPV16 e6/E7的锤头状核酶(hammerhead ribozyme)已被证实可在某种程度上抑制体外培养的肿瘤预防细胞抑郁的生长. 另一种发夹状核酶(hairpin ribozyme)则被认为是极具潜力的抗病毒咨询制剂. 设计合成的抗HPV16的发夹状核酶在体外试验中具有很高的催化裂解靶mRNA的效率;
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nucleoside diphosphatase：核苷二磷酸酶
核苷 nucleoside,riboside | 核苷二磷酸酶 nucleoside diphosphatase | 核苷焦磷酸酶 nucleoside pyrophosphatase
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Ribozyme Mechanisms：核酶机制
Ribosome Structure核糖体结构 | Ribozyme Mechanisms核酶机制 | Ribozymes and Evolution核酶和进化
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Ribozyme Structural Elements: Group I Introns：核酶结构元件:I型内含子
Ribozymes and Evolution核酶和进化 | Ribozyme Structural Elements: Group I Introns核酶结构元件:I型内含子 | Ribozyme Structural Elements: Hairpin Ribozyme核酶结构元件:发夹型核酶
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Ribozymes and Evolution：核酶和进化
Ribozyme Mechanisms核酶机制 | Ribozymes and Evolution核酶和进化 | Ribozyme Structural Elements: Group I Introns核酶结构元件:I型内含子
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ribozyme：核酶
3.5 催化作用 近几年来发现某些RNA具有酶的催化功能,这类具有酶活性的RNACech将它命名为"核酶"(ribozyme). 最早发现某些低等真核生物的细胞核rRNA前体及线粒体mRNA前体和rRNA前体在除去自身居间顺序(IVS)时是自我催化进行的.
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ribozyme：核酶基因
另外,Han等将一个核酶基因(ribozyme)融合到一段能与水稻矮缩病毒(Rice dwarfvirus,RDV)5号片段杂交的序列后转入粳稻Tongling 1号,转基因植株表现高度抗病.
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axehead ribozyme：斧头(状)核酶[见于delta肝炎病毒]
avirulent strain减毒株,无毒株 | axehead ribozyme斧头(状)核酶[见于delta肝炎病毒] | axenic cultivation纯性培养
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axehead ribozyme：斧头(状)核酶
avirulent strain 减毒株,无毒株 | axehead ribozyme 斧头(状)核酶 | axenic cultivation 纯性培养
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chimeric ribozyme：嵌合体核酶
嵌合启动子:chimeric promoter | 嵌合体核酶:chimeric ribozyme | 嵌合分子:Chimeric molecular