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The amount of vimentin IF per cell was higher than those of tubulin and F-actin at 12-24h of culture, but it broken down and decreased steadily when the cells became differentiated into late erythroblast at 36-48h of culture, thus facilitated the eccentric nucleation which we regard it as the initial step of denucleation. Both of the fluorescence intensity of tubulin and actin exhibited a significant rise and aggregated between the extruding nucleus and incipient reticulocyte before and during denucleation, and finally play a role in the commitent to ennucleation, the second phase of denucleation.
在培养24小时前，每个细胞中波形纤维含量均高于同期微丝和微管蛋白含量，但在培养24-36小时的晚幼红细胞阶段，波形纤维蛋白降减和解聚，为核偏位创造了条件，这是排核的第一阶段，而随后微丝和微管蛋白的荧光染色强度则有所增加，并逐渐聚集在外排中的核与预期形成网织红细胞之间的缢痕区，在将细胞核排出的排核第二阶段起主要作用。
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ResultsMorphological features with diagnostic value were established as follows:①Irregular or round-mesh basket type nucleolus;②Malformed nucleus and lobate nucleus with fine bridge;③Perinuclear microfilaments;④Nuclear bodies;⑤Nuclear inclusion bodies;⑥Marked cell pleomorphism arranged irregularly,like a disordered stone pavement;⑦Strange cell and/or giant cancer cell were found;⑧Peculiar papillae or cell clusters were formed;⑨Specific function structure on the surface of cell differentiated poorly.
结果 确定了具有诊断意义的形态学特征：①不规则奇形核仁或网孔状核仁；②畸形核，分叶核伴细桥－核间桥形成；③核旁微丝；④核体；⑤核包涵体；⑥细胞重度多形性，排列紊乱，似乱石铺堆；⑦出现奇形细胞和/或癌巨细胞；⑧形成奇形乳头和奇形细胞簇；⑨细胞表面特异功能结构分化不良。
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A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.
研究发现，次生壁形成早期，细胞核具有双层核膜，染色质凝聚，可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中；随后，双层核膜消失，细胞器将逐渐解体，多泡体开始出现在纤维细胞的细胞质；随着年龄的增加，纤维细胞壁逐渐增厚，并出现多层结构现象，而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。
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The injured gill cells had a different ultrastructure, with endoplasmic reticulum dilated and vesiculated, mitochondrial cristaes partially disintegrated, gill fibre cuticles denatured and dropped. The ultrastructural damages to hepatopancreatic cells were characterized by the decrease of microvilli, swelling and breaking up of mitochondria, expansion of endoplasmic reticulum, disruption and vacuolization of nucleus, tumefaction of nuclear membrane and increase of lipid droplets. As for the toxicological changes of myocardial cells, the mitochondria were swelling, having a lot of vacuolus and disintegrating, while the myofibrils were irregular with endoplasmic reticulum dissolved.
鳃细胞的损害表现为鳃丝水肿，细胞器溶解，角质层变薄，线粒体、内质网的肿胀、解体；肝胰脏细胞的主要损害特征为肝管微绒毛减少，线粒体水肿解体，内质网扩张，细胞核空泡化，核膜水肿和脂肪滴增加；心脏细胞的毒理变化为线粒体内嵴肿胀、瓦解，肌原纤维不规则，内质网溶解。
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Before treatment,there are abundant microvtlli on the cell surface,many filoplodia on the cell rim,few of endoplasmic reticulums , Golgi complexes and mitochondrions with abnormal structure and lots of free ribosomes in the cytoplasm. The shape of nucleus is irregular with high nuclear-cytoplasmic ratio, many dark pellets of heteromatin and a few of nucleoluses in which there ar...
经1.5×10~2g/L地塞米松处理后，细胞表面微绒毛和丝状伪足显著减少或消失，出现皱褶状和小泡状结构；细胞核形规则，核质比值减小，异染色质团块减少，核仁数目减少、结构致密；细胞质中粗糙型内质网、高尔基体、线粒体数量增多，形态结构典型，游离核糖体减少，这些变化说明地塞米松改变了MGc80-3细胞的超微结构特征，具有明显的诱导分化作用。
- 更多网络解释与核网丝相关的网络解释 [注：此内容来源于网络，仅供参考]
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capillitium：孢丝
孢子囊柄伸入囊内的部分,称囊轴(columella),囊内有孢丝(capillitium)交织成孢网. 然后原生质团中的许多核进行减数分裂,原生质团割裂成许多块单核的小原生质,每块小原生质分泌出细胞壁,形成1个孢子,藏在孢丝的网眼中. 成熟时,包被破裂,
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centromere plate：着丝粒板
nuclear reticulum 核网 | centromere plate 着丝粒板 | chromatoid body 拟染色体
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karyomitome：核网丝
karyomicrosome 核微粒体 | karyomitome 核网丝 | karyomitosis 有丝分裂
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nuclear reticulum：核网
microcentrum 中心粒团 | nuclear reticulum 核网 | centromere plate 着丝粒板
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karyomicrosome：核微粒体
karyometry 核测定法 | karyomicrosome 核微粒体 | karyomitome 核网丝
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karyomitosis：有丝分裂
karyomitome 核网丝 | karyomitosis 有丝分裂 | karyomorphism 细胞核形态