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HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.
HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的,尽管硫氧还蛋白有很强的助溶作用,该蛋白依然不可溶形式存在;NS5B—dc21蛋白通过C末端疏水序列的删除,获得了可溶表达;NS5AB蛋白以不可溶形式表达,需要复性。
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It objects the literary theory of neo-classicalism arid is greatly influenced by the pantheism of naturalism. Emotionalism has greatly promoted 19th century Romanist poetry, which is expressed in the immanent amalgamation of aestheticism with the "deity" of nation.
它反对新古典主义文论,并受到自然主义泛神论的极大影响,对19世纪英国浪漫主义诗歌有很大的促进作用,集中表现为审美主体与自然的"神性"的内在融合。
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Results A significant decrease in the three-dimensional kinematics of talonavicular joint was observed(P〈0.01)in cadaver model following calcaneocuboid arthrodesis.
结论跟骰关节融合对距舟关节三维运动度有较大的限制作用,临床应用时不可忽视此负面效应。
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Majority of acute leukemias in infant, either acute lymphoblastic leukemia or acute myeloblastic leukemia, posses a chromosomal translocation affecting the 11q23 chromosome region which specifically inoles the mixed-lineage leukemia gene.1-3 Most pediatric leukemias with MLL rearrangement clearly hae a remarkably short latency.1,4 MLL gene rearrangement is also associated with secondary leukemias of patients preiously treated with the topoisomerase II inhibitors.4 The latency of these secondary leukemias is similarly ery short.4 Of note, the concordance rate of leukemia with MLL rearrangement in infant monozygotic twins approximates to 100%,1,4 and identical breakpoint in the MLL gene was shared in these pairs of identical twin infants with concordant ALL.1,4 Moreoer, the unique and clonotypic MLL fusion gene was detectable in neonatal blood spots for Guthrie cards from non-twined indiiduals who subsequently deeloped ALL.1,4 These obserations indicate not only that MLL fusion is generated in utero but also that MLL fusion proteins could be capable of inducing leukemic transformation with few, if any, secondary mutations.2,3,4 Greaes et al speculate that an MLL fusion protein somehow promotes rapid transition to full-blown disease in patients ia ery rapid clonal expansion, genetic instability, or inhibition of DNA damage repair.4 In general, for clonal expansion of malignancies, tumor cells often hae acquired strategies that escape immune sureillance of the hosts.5,6 Immune escape mechanisms also contribute to the failure of graft-ersus-leukemia effect after allogeneic hematopoietic stem cell transplantation.7 Therefore, leukemia cells could acquire some immune escape mechanisms during leukemogenesis.
绪论 绝大多数的婴儿白血病,不管是急性淋巴性白血病或是急性骨髓性白血病,在染色体11q23部位有染色体易位的情况;这个部位的染色体易位牵连了混合谱系白血病基因。大多数具有MLL基因重排的儿童白血病潜伏期明显短很多。MLL基因重排也和经拓扑异构酶II抑制剂治疗后的继发性白血病有关。这些继发性白血病的潜伏期类似地都非常的短。很重要的是,单卵双胞胎婴儿同时患有或同时免于MLL基因重排阳性的白血病的一致性接近100%;并且同样患有ALL的同卵双胞胎的MLL基因的断裂点是一致的。而且,这种独特的克隆特异性的MLL融合基因能够从那些得ALL的非双生个体出生时的血斑标本中检测到。这些发现表明MLL融合基因产生在胎儿还在子宫的是后,而且MLL融合蛋白能过和其他的基因突变一起诱导白血病的产生。Greaes 等推测MLL融合蛋白在某种情况下同过快速克隆增殖,遗传的不稳定性或是DNA损伤修复的抑制促使疾病迅速地全面爆发。恶性肿瘤细胞的克隆增殖通常已经获得了逃避机体免疫监视的能力。免疫逃避机制也归因于异体外周血干细胞移植后移植物抗白血病作用的失效。所以,白血病细胞在白血病的产生过程中可能获得了某些免疫逃脱机制。
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By the affinity chromatography,fusion protein is purificated. The antiserum of chicken and rabbit were prepared with the fusion protein and Ghrelin-KLH.With active immunity and passive immunity,confirmed that Ghrelin of chicken have no obviously impact on ultimum metabolite,plasma Insulin,glucagons, T_3 and T_4.It entrances plasma GH,but inhibit food intake and increase of fat and body weight.These conclusion are obviously different from the mammal.
以纯化的融合蛋白和人工合成的Ghrelin-KLH为抗原,制备了鸡、兔抗血清:通过主动免疫、被动免疫中和实验证实禽类Ghrelin对鸡血浆中代谢产物、胰岛素、胰高血糖素、甲状腺素浓度没有明显的影响,对GH的分泌有促进作用,但与哺乳动物中不同,Ghrelin抑制鸡的脂重、体重的增加,抑制鸡的摄食。
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Unfortunately, there's little you can do to encourage squad gelling.
不过不幸的是,你的激励对球队融合基本么有什么作用。
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Berbamine could inhibit the growth of leukemia cell line NB4. The induction of cell apoptosis may be one of the mechanisms for suppressing the growth of leukemia cell line NB4. Inhibition of Survivin mRNA and upregulation of Caspase 3 protein might be also involved in cell apoptosis.
小檗胺对NB4细胞有增殖抑制作用,诱导细胞凋亡可能是其抗肿瘤作用的重要机制之一,但不是通过作用于NB4细胞特异性融合基因PML/RARα发挥作用的,其抗凋亡的可能分子机制之一是抑制Survivin基因的表达和增加Caspase3的表达。
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On the one hand, there are lots of association rules theory of application-oriented is waiting for explore and creative in these big theory framework; on the other hand, with the development of data mining and relevance technology, it must will birth new mining theory, and it will have impetus to some particular application, it may merge particular application target, such as marketing, custom relationship managements electronic business.
一方面,在这些大的理论框架下有许多面向实际应用目标的关联规则挖掘理论等待探索和创新;另一方面,随着数据挖掘技术本身和相关技术的发展,新的挖掘理论的诞生是必然的,而且可能对特定的应用产生推动作用,可能融合特定的应用目标,如市场营销、CRM、电子商务等。
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Cell-cycle synchronization between the donor cell and recipient oocyte determines the embryo development in nuclear transfer. In the present study, we microinjected primary spermatocyte into the perivetelline space of oocyte. 37% pairs fused after electric stimuli and the resulting oocytes were culture for 2 h in MEM with or without CB.
在本研究中,我们将小鼠的初级精母细胞显微注入MI卵母细胞的透明带下,经直流电脉冲作用后有37%的初级精母细胞融入卵母细胞,然后将融合的卵母细胞分成两组在MEM和含有CB的MEM培养液中分别培养,2小时后将在CB培养液中培养的卵母细胞转入正常培养液中。
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The effects of curcumenol on induction of differentiation of K562 cell line may be due to its prevention of chromosome damage and decreasing of bcr/abl gene mRNA expression.
莪术醇对畸变的K562细胞染色体有作用,影响bcr/abl融合基因的表达,使K562细胞向成熟分化。
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bottle:瓶
FABBRI保鲜液FABBRI保鲜液混合了葡萄糖浆、柠檬酸、抗氧化剂等多种成分融合而成,具有保质、保鲜作用,让产品更加新鲜有光泽,保质期限更耐久. 是保存水果、蔬菜的好帮手. 产品类别:其他产品(PTHERS)产品名称:保鲜液产品(英文)名称:CHIARAFRUTTA产品编号:FB9964009-K19产品产地:意大利(ITALY)产品规格:900g/瓶(bottle).
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coated vesicle:有衣小泡
通过细胞内吞作用(endocytosis) ,将复合物包封于有衣小泡(coated vesicle) 内. 进入细胞质后,有衣小泡形成内吞小泡(endosome),与其它细胞内囊泡融合,又将内吞物转送到溶酶体(lysome)内,实现内吞作用介导的脂质体与细胞的融合.
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Aspidisca costata:有肋楯纤虫
腹面扁平,纤毛融合成触毛,分布在腹面一定的地区,又分前触毛,腹触毛,臀触毛,尾触毛和缘触毛,用触毛支撑虫体,负有"足"的作用.观察触毛在虫体的分布可区别其种类.常见种类有肋楯纤虫(Aspidisca costata),尖毛虫(Oxytricha),
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Oligochaeta:寡毛纲
环节动物门(annelida) 寡毛纲(oligochaeta) :寡毛纲约有3000 种蚯蚓,但只有少部分为淡水生. 蚯蚓的体呈长圆筒形,由若干体节组成. 由于蚯蚓体内具有丰富的酶系统,使得被吞食的物质经过2 - 3h体内酶的作用就形成了高度融合的有机无机复合肥--蚓粪[52 ] .