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Based on the genome conservative sequence of tabacoo mosaic virus, cucumber mosaic virus and potato X virus, special primers were designed respectively. With the RT-PCR, the target fragments about 313,326 and 392 bp were ampilfied separately from the infected sample, but not health sample.
设计烟草花叶病毒、黄瓜花叶病毒和马铃薯X病毒的特异性引物，RT-PCR检测，从感病叶片中分别扩增出313，326，392 bp的目的片断，而健康叶片中无此扩增带。
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Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.
本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。
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There were no significant differences in the relative consumption rate and approximate digestibility between the 6 instar larvae reared with two tobacco species.
26龄幼虫对两种烟草的相对取食量和近似消化率无显著差异，但是对黄花烟草的利用率、转化率及其相对生长率显著下降。
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Put the spotless potatoes, tomatoes and toba cco atoms into the hot pot.
把无斑点的土豆，番茄和烟草微粒放进热锅里。
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The vast majority of adult smokers taking up the habit as teens, and are more attracted to flavored cigarettes and tobacco products than to unflavored ones, according to the FDA, which is also mulling over regulating menthol cigarettes and flavored tobacco products other than cigarettes, the agency said.
根代理商说，据禁烟中心的调查，大部分成年人吸烟的习惯是青少年时期养成的，相对于无香味的烟草来说，他们更容易被有香味的烟草吸引，同时禁烟中心还
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The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.
其中甲醇提取物的抑菌活性较乙酸乙酯好，在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用，抑制率在62.9%~85.1%；抑制孢子萌发试验表明：菌丝体甲醇提取物对烟草赤星病菌的毒力较好，其EC50仅为62.5328μg/mL；抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用，而对大肠杆菌无明显的抑制作用。
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Oral HP infection is strongly associated with oropharyngeal cancer among subjects with or without the established risk factors of tobacco and alcohol use.
在有或无烟草和酒精已确定风险因素的受试者中，口腔人乳头状瘤病毒感染与口咽癌高度相关。
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The results from insect bioassay with peach aphid showed that the transgenic plants of pBI121AVA-GUS and pBI121AVAc-GUS were aphid resistant, evidenced by a 60.81%~50.63 reduction in insect population density, some plants were more than 97%.
抗蚜实验表明含内含子和无内含子的AVA基因在转基因烟草中的抗蚜能力不同，转AVA和AVAc烟草对蚜虫的抑制率分别为60.81%和50.63%，有的植株高达97%。
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The recombinant plasmid pBI121-VP6 was transformed into Agrobacterium tumefaciens strainLBA4404 by freeze-thawing method and the introduced into the sterile tube culture shoot of tobacco NC89 and baoding alfalfa by leaf disc infection method.
以冻融法将pBI121-VP6重组质粒导入根癌衣杆菌LBA4404，用叶盘法转化于NC89烟草和保定紫花苜蓿的无菌试管苗。
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PHap1 was first transformed into Agrobacterium tumefaciens strain LBA4404 by electroporation first and then introduced into the sterile tube culture seedlings of different susceptible potato eultivars'Mira','Zhong-5-1'and'Jinguan', and tobacco cultivar NC89, respectively, by the leaf disc infection method.
以电激法将pHap1导入根癌农杆菌LBA4404，用叶盘法分别转化马铃薯青枯病感病品种米拉、中-5-1、金冠以及烟草感病品种NC89的无菌试管苗。
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roasting：焙
经高温烘焙(Roasting)后,烟草的性质得以调校,变得更加圆熟和圆润,并带股浓郁的坚果味和细致的芳香. 调味Burley抽起来,早段芳醇,无刺激,且有股微微的坚果味. 若烟草本身质量好,并经适当处理的话,烟草会产生出饱满、香郁的风味,
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shebeen：下等酒店;无执照的酒店
sheathing护套;铠装;外壳;覆盖层;外膜 | shebeen下等酒店;无执照的酒店 | shedburn(烟草)棚焦
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shed burn：(烟草)棚焦
shebeen 下等酒店;无执照的酒店 | shed burn (烟草)棚焦 | shed damage (烟草)晾干时的损伤