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At last, the genetic relationship between Brassica genera and Arabidopsis thaliana was summarized.
最后，文章对芸薹属不同基因组和拟南芥基因组之间的亲缘关系进行了综述。
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We retrieve the amino acid sequence by BLAST software in NCBI server to compare the homology and found that in GenBank+EMBL+DDBJ+PDB there are 100 sequence have the homology with this sequence, these 100 sequence are all sequence of PI. Among this the Amaranthus hypochondriacus have the maximum homology, then the Cucurbita, Arabidopsis thaliana, Linum, Euphorbiaceae, etc.
将BPI-2基因所推导的氨基酸序列在NCBI服务器上用BLAST搜索软件进行同源性基因检索，结果在GenBank+EMBL+DDBJ+PDB数据库中显示100条有同源性的序列，这些序列均为蛋白酶抑制剂序列，其中同源性较高的依次是苋属、南瓜属、拟南芥、亚麻属、大戟属等，相应氨基酸序列的同源性在分别为50％～63％之间。
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Sequence analysis showed that the complete CDS encodes a putative Rab5B protein with 199 amino acid residues and have high similarity to other three plant Rab proteins: LjRab5B from Lotus japonicus, McRab5B from Mesembryanthemum crytallinum and Ara6 from Arabidopsis thaliana.
序列排比分析表明，它与百脉根属的Rab5B蛋白(LjRab5B)，、龙船海棠属Rab5B蛋白(McRab5B)和拟南芥的Rab5B蛋白(Ara6)有很高的序列相似性，分别达到78％，79％和77％的一致值。
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The concrete work include: First, we consult a lot of herbarium specimen from PE, NAS, IFP and XJA etc.; Second, we collect more than 100 species of Brassicaceae from Xinjiang, Jilin and Shandong; Third, we study the phylogeny of Brassicaceae from China to approach some divergence on the tribe, genera and species of Brassicaceae using many ways including morphology, micromorphology and molecular systematics, furthermore, estimate the significance of every index.
2确定了两种模式植物-拟南芥与盐芥的系统学位置和系统学关系，认为两者分别归于拟南芥属和盐芥属，其亲缘关系较远，拟南芥属靠近须弥芥属、荠属、亚麻荠属、旗杆芥属、无苞芥属、糖芥属以及垂果南芥等，而盐芥属靠近山嵛菜属和沟子荠属，对于它们的深入认识，为两种模式植物在进化领域研究物种抗逆适应与趋异分化提供了一个不可多得的比较模式系统，以及更多可供比较的物种，具有重要的应用价值。
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In model plant Arabidopsis thaliana, also from Brassicaceae, the TRANSPARENT TESTA 12 (TT12, AtTT12) gene is seed coat-specific and encodes a vacuolar flavonoid/H+ antiporter, transporting cytoplasm-synthesized glycosylated flavan-3-ols to the vacuoles for further polymerization into seed coat pigments.
同属十字花科的模式植物拟南芥中，种皮特异表达的TT12（透明种皮12，AtTT12）基因编码一种液泡型类黄酮/H+-逆向转运蛋白，将胞浆内合成的糖苷化的黄烷-3-醇转运到液泡中，供进一步聚合形成种皮色素。
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In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.
为了从分子水平阐明十字花科植物间的亲缘进化关系，给植物种质资源的创建提供理论依据，试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物，运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列，经比较分析，结果表明：这些同源序列的相似性达80%以上，所推导的氨基酸序列相似性达70%以上，且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%，属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%；由氨基酸序列构建的分子系统树可知，在亲缘进化关系上芸薹属与萝卜属较近，其他依次为蔊菜属、拟南芥属、荠菜属，而与诸葛菜属最远。
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Experiment was conducted with cpSSR primers obtained from Arabidopsis thaliana for studying the germlasm of genus Castanea Mill. The optimization of main influential factors of cpSSR reaction system in Castanea was studied.
用1对来源于拟南芥的cpSSR引物研究栗属资源，对栗属植物cpSSR反应体系的主要影响因子进行了优化筛选。
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Arabidopsis：鼠耳芥属
十字花科(Brassicaceae) 鼠耳芥属(Arabidopsis) 拟南芥(A. thaliana)microRNA(miRNA)是拟南芥研究中近几年来最值得注意的热点之一. miRNA是高等真核生物中一类非翻译RNA,由基因组编码. miRNA前体的转录过程与普通基因mRNA的转录过程基本类似.