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Methods: Adopting pig bile culture medium, the inhibiting or killing effects on pig bladder worm in vitro were observed;by using the methods of xylene-induced ear inflammation in mice and granuloma swelling induced by cotton ball in rats, the effects of anti-inflammation were observed;by using the methods of body twisting and hot-plate in mice, the effects of analgesia were observed. Through the mice auricle microcycle, the rats thrombus law in vitro and bleeding time, clotting time determination to the mice, the function of invigorating the blood were observed;adopting the penicillin abdominal cavity injection method, the mice epilepsy model were prepared, and epilepsy mice brain organization MDA content and SOD, ATPase vigor be measured by using chemical colorimetry.
利用猪胆汁培养基观察该方在体外对猪囊尾蚴的抑制或杀灭作用；采用二甲苯所致小鼠耳肿胀法、大鼠棉球肉芽肿法以观察该方的抗炎作用；采用热板法和化学刺激扭体法观察该方的镇痛效果；通过小鼠耳廓微循环法、大鼠体外血栓法和对小鼠出、凝血时的测定观察该方的活血作用；采用青霉素腹腔注射法制备小鼠癫痫模型，并利用化学比色法检测癫痫小鼠脑组织中MDA的含量及SOD、ATPase的活力。
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In this thesis, drug distribution in local tissues after topical administration of the ATPs was investigated with HPLC technique, concentration of ACF in skin and muscle remained high during 12hours. Pharmacodynamic of ATPs was evaluated with diclofenac gel as a control, and it was found that ATPs had stronger anti-inflammatory activity than diclofenac gel.The results of rabbit skin irritation test and Guinea pig skin allergia test make it certain that ATPs is safe for topical administration.
本文利用局部组织活检法考查了大鼠局部应用ATPs后的局部组织分布情况，结果表明药物能在皮肤和肌肉组织中12h维持较高的药物浓度；将所制ATPs与市售双氯芬酸钾凝胶进行了药效学比较，结果表明ATPs的抗炎效果优于英太青；家兔皮肤刺激性试验结果表明，ATPs对完好皮肤无刺激性，对破损皮肤具有轻度刺激；豚鼠过敏性试验结果显示ATPs无致敏性。
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The results demonstrated that supernatant IL-6(181±22U/ml) may be found after cultivation of unstimulated HUVEC for 24 hours.
HUVEC原代培养，HUVEC释放IL-6、IL-8观察，MTT比色法作IL-6活性测定，双抗夹心ELISA法测IL-8，t检验进行统计学处理。结果未刺激的HUVEC培养24小时上清IL-6活性为 181±22U/ml。
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Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.
(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。
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contrastimulation：抗刺激法 抗兴奋疗法
contrastimulant 抗兴奋剂 抗兴奋药 | contrastimulation 抗刺激法 抗兴奋疗法 | contrastimulism 抗刺激法 抗兴奋疗法