抑菌测定

基本解释 (translations)

bia

与抑菌测定相关的网络例句 [注：此内容来源于网络，仅供参考]

The bacteriostatic effects, action mechanisms and antimicrobial spectrum of the strains were tested. After 5 days of culture, a bacteriostatic belt of 6.9-9.67 mm in width was observed on the culture plate, and the inhibitory activity maintained for 10 d. Prefiltration fermentation of the strains P1 and P5 could significantly inhibit conidial germination of the pathogen, with inhibitive rate ranging from 97.51% to 98.12%. The action might be realized by inducing hyphal deformity, bioplasm concentration, conidial germination inhibition and distorting germ tubes of conidia.

通过对其抑菌效果、机制和抗真菌谱进行测定，结果表明，P1、P5和葡萄灰霉病菌对峙培养5 d后，均产生明显的抑菌带，抑菌带宽度为6.90～9.67 mm，培养10 d后，仍然保持稳定的抑菌效果；P1、P5的发酵原液对葡萄灰霉病菌孢子萌发具有显著的抑制作用，抑制率为97.51%～98.12%；P1、P5对病菌的作用机制表现为使病菌菌丝畸形、原生质浓缩、菌丝断裂、抑制病菌分生孢子的萌发、导致孢子芽管扭曲。

The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.

测定了它们对自由基的清除率和对四种细菌的抑菌效果，研究结果表明，总色素和褐藻黄素的浓度与自由基清除率成正比，当总色素浓度为0.52mg.ml~(-1)时，清除率达到65.819%，高于陆生植物黑米色素和紫甘薯色素；当褐藻黄素浓度为0.27mg.ml~(-1)时，自由基清除率达到19.85%，低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素；对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示，海带总色素对金黄葡萄球菌的抑菌活性最强，抑菌圈直径为18.9mm；褐藻黄素对八叠球菌的抑菌活性相对较强，抑菌圈直径为10.2mm。

Utilization ofantiseptic would not be affected by temperature or pH.The antiseptics had a longperiod of validity,the effect is much better than most other antiseptics,could notarose skin hypersusceptibility,resist to metabolization,and had a wide utilizationrange.

研究中采用了本实验室自行合成的抗菌剂反丁烯二酸桂醇甲酯，测定了反丁烯二酸桂醇甲酯在不同的pH值、不同温度、不同浓度条件下的抑菌活性、抑菌谱，并与其它常用抗菌剂的抑菌效果做了比较。

S extract had an inhibition effect against Sphaerotheca fuligenea,The inhibition rate was 100% when extract concentration was 20mg/mL and the inhibition rate got to 67.66% even at the concentration of 2.5mg/mL. The extract of E. humifusa had different preventive effects against four strains of S. fuliginea from four districts in Shanghai. The preventive effects on the Qibao and Baoshan were better than those of Nanhui and Qingpu. The microorganism inhibition experiment by filter papers indicated that the extract of E. humifusa had higher inhibition effect against bacteria than on pathogenic fungus. Diameters of the zone of inhibition of salmonellas, Echerichia coli and Staphylococcus aureus were 15.77mm, 12.27mm and 10.53mm respectively. The extract of E. humifusa had a certain effect against Colletotrichum lagenarium, Botrytis cinerea, Rhizoctomia solan and Pyricularia oryza but no effect against Helminthosporium maydis and Gibberella zea. The 4 cation exchange resins and ethanol deposition were used to isolate the active fractions from supernate after alkali deposition.

提取液对黄瓜白粉病菌体外分生孢子萌发抑制实验表明地锦提取液对黄瓜白粉病菌分生孢子的萌发有很好的抑制作用，抑制率随着地锦提取液浓度的增大而增高，当浓度为20mg/mL时，抑制率达到100%，即使在浓度为2.5mg/mL时，抑制率也达到了67.66%；室内盆栽苗期预防性实验表明了地锦提取液对上海4个不同地区的黄瓜白粉病菌均具有不同程度的抑制效果，对七宝和宝山地区的菌株抑制效果优于南汇和青浦地区的菌株；用滤纸片法测定了地锦提取液的抑菌谱，实验结果表明地锦提取液对供试的4种植物病原真菌：黄瓜炭疽病菌、黄瓜灰霉病菌、水稻纹枯病菌和水稻稻瘟病菌均具有一定的抑制效果，而对玉米小斑病菌和小麦赤霉病菌没有抑制效果，对供试的3种人类致病细菌沙门氏菌、大肠杆菌和金黄色葡萄球菌均具有很好抑菌效果，抑菌圈直径分别为：15.77mm， 12.27mm和10.53mm。

The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.

实验部分采用以石油醚脱脂，甲醇为溶剂的回流提取法来提取植物多酚，用显色反应检识酚羟基；采用紫外分光光度法对多酚粗体物进行总多酚含量的测定；选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌，采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验，并测定其最低抑菌浓度；采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析，根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

The inhibitory tests in vitro were performed against Escherichia coli, Salmonella pullorum with filter paper method and tube dilution method. And the diameters of inhibition zone, minimal inhibitory concentrations of levofloxacin combined with polymyxin E were measured.

方法］应用纸片法和试管稀释法进行体外抑菌试验，测定左氧氟沙星与多黏菌素联合对鸡大肠杆菌、鸡白痢沙门氏菌的抑菌圈大小和最小抑菌浓度。

The results showed that the three disparate treated substances have no visible antibacterial effect to E. coli , but a obvious effect to Staphylococcus aureus with inhibition of about 10 bacteriostasis units; have dissimilar effects to 10 kinds of fungi among the total kinds, and have a good inhibitory effect to Cytospora.coccodesHughes with fungistasis rate above 65%. After the gel filtration and activity detection, 50mM ammonium acetate was screened out as the more suitable eluents, the crudes were separated into strikingly unlike parts with obviously different antimicrobial activity.

结果表明，大蜡螟抗菌物质对大肠杆菌没有明显的抑制作用，而对金黄葡萄球菌抑制作用明显，抑菌活性单位在10左右；对10种植物病原真菌均有不同的抑菌活性，其中对苹果树皮腐烂病菌的抑制率达到65%以上；经过凝胶过滤分离，及对分离组分的抑菌活性测定，筛选出较合适的洗脱液为50mM乙酸铵，各分离组分抑菌活性差异明显，粗提物被分成了两个差异显著的部分。

Through measuring MIC and bacteriostasis diameter, the inhibited abilityof antimicrobials to bacterium and funguses is assessed. Through measuring optical density, inhibited ability of single antimicrobials to algaes si assessed. And, the variation law of antibacterical effect of antimicrobials is researched with variation of time and pH.

通过测定最低抑菌浓度MIC值和抑菌圈直径考察单体抗菌剂对菌类的抑制能力，通过对水样光密度的测定考察单体抗菌剂对藻类的抑制能力，并研究抗菌剂抗菌效果随时间及pH值的变化规律。

MethodsMinimum inhibitory concentrations of NAC,piperacillin,ciprofloxacin,and amikacinagainst P.aeruginosa were determined by the broth microdilution test.Interactions of NAC and above antibiotics were determined using chequerboard MIC assay.

方法应用微量肉汤稀释法测定NAC、哌拉西林、环丙沙星、阿米卡星对铜绿假单胞菌的最低抑菌浓度：应用棋盘法测定NAC与上述三种抗菌药物的联合抑菌效应。

与抑菌测定相关的网络解释 [注：此内容来源于网络，仅供参考]

xanthene：吨

文章摘要:目的测定六种呫吨(xanthene)化合物的光敏抗菌活性以及它们的物理化学性质,评价两者之间的联系. 方法通过测量最低致死浓度(MLC)和Daniel抑菌圈法测定六种呫吨化合物的光敏抗菌活性;采用对-亚硝基二甲基苯胺(RNO)脱色反应检测单线态氧产率.

dyes：色素

我们会在培养基中加一些色素(dyes)或(及)受质(substrates)以确定其代谢状况或分解能力.有时我们也会在培养基上涂下大量的特定菌种来分离具有抑菌或杀菌能力的菌株.为了测定突变剂或致癌物质的存在,

aflatoxin：黄曲霉素

研究这两种精油的抗真菌活性是专门针对抑制寄生曲霉(Aspergillus para siticus)的生长和黄曲霉素(aflatoxin)的产生而言的. 对精油的最低抑菌浓度(MIC)和最低杀真菌浓度(MFC)进行了测定. 上述精油对寄生曲霉的静态作用是250ppm,

Candida krusei：克柔念珠菌

目的 应用美国国家临床试验标准化委员会(NCCLS)推荐的微量法测定山苍子油(Lisea cubeba oil)对5种医学重要念珠菌的最小抑菌浓度,并在电镜下观察山苍子油作用后,耐唑类药物的克柔念珠菌(Candida krusei)超微结构的改变,探讨其作用机制,