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Result: It has been found out that the fat-soluble nonsaponified extract from Temminck et Schlegel and the alcoholic extract from Syngnathus acus have cytotoxic activities.The nonsaponified extract from Temminck et Schlegel can inhibit the grouth of cancer cell strains KB,Hela,PAA,K562,and Bcap37,and the alcoholic extract from Syngnathus acus can inhibit the growth of cancer cell strin KB,But Bloch shows no apparent anticancer activity.
结果:尖海龙的95%乙醇提取物对KB细胞的生长具有抑制作用,并呈量效关系;粗吻海龙的脂溶性非皂化物具有不同程度的抑制人癌细胞株KB,Hela,Bcap37,K562,PAA细胞的作用,并有明显的量效关系;拟海龙的各提取物未见有明显的抑制肿瘤细胞生长的作用。
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Results: The extracts of ethanol, petroleum ether and ethyl acetate of Rhizoma Amorphophalli have inhibitory effect on the proliferation of cell SGC-7901, and the extract of petroleum ether has much more evident effect, showed a dose-effect curve.
结果:蛇六谷醇提取物、石油醚萃取物和乙酸乙酯萃取物均具有一定的抑制人胃癌SGC-7901细胞增殖的作用,以石油醚萃取物的抑制作用最为明显,呈现较好的剂量-效应曲线。
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Parthenolide is extract from feverfew, it's main component is sesquiterpene, it is commanly used in west country and is mainly used to therapy skin infection, rheumatic disorder, migraine. But recent research indicate that parthenolide can regulate many pathway of signal in the cell, inhibit the cyclooxygenase, TNF-α, interleukin-1 expression induced by lipopolysaccharide in macrophage, inhibit the iNOS expression in human monocyt, inhibit the activity of NF-κB in Hela cell, inhibit the growth of tumor cell and can relieve the myocardial ischemical reperfusion injury and so on. But the invention research about the effection of parthenolide on the VSMC is very rare in the world, so we do some research about the effection of inhibition of parthenpolide on the proliferation of VSMC and the signal transduction mechanism.
小白菊内酯(parthenolide,又名Sesquiterpene lactone)是艾叶菊属黑叶母菊的提取物,主要成分是倍半萜烯内酯,西方国家较为常用,主要用于治疗皮肤感染、风湿病和偏头痛,但近年来发现小白菊内酯能调控细胞内多种信号途径,如抑制巨噬细胞中脂多糖诱导的Cox,肿瘤坏死因子α和白细胞介素-1的表达,能抑制人单核细胞中iNOS的表达,能抑制Hela细胞中NF-κB的活性,抑制肿瘤细胞的生长,同时能减轻心肌缺血再灌注损伤等,但小白菊内酯在VSMC中的干预研究目前国内外非常罕见,为了确切评价小白菊内酯对血管平滑肌细胞增殖的抑制作用及相关的信号转导机制,我们进行了一些研究。
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Porphyrin nitrogen mustard has distinctive effect on the synthesis of DNA and photodynamic anti-tumor activity.
卟啉氮芥可显著抑制人肝癌细胞SMMC7721DNA的合成;对人肝癌细胞SMMC7721有显著的光动力抑制作用,可破坏肿瘤细胞的细胞膜,抑制肿瘤细胞DNA的合成。
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Preliminary study revealed the devascular effect of PYM correlated with the inhibitory of vascular endothelial cells.
一、PYM抑制人脐静脉内皮细胞生长的体外研究目的通过体外实验观察PYM对人脐静脉内皮细胞株一一一石CV304细胞生长、增殖和凋亡的影响,以证实PYM对血管内皮细胞的抑制作用及了解相关的影响因素。
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In conclusion ,the results of this study showed that the inducing agent HMBA could effectively inhibit the proliferation activity of the human ostosarcoma MG-63 cells,cause the arrest of G0/G1 phase, reverse the malignant phenotype characters,upregulate expression of the osteoblat-like phenotype markers including collagen,osteocalcin,osteonectin , facilitated the formation of bone nodule .
本研究的结果表明HMBA能够有效抑制人成骨肉瘤MG-63细胞增殖活动,改变肿瘤增殖核抗原表达,将细胞周期阻滞在G0/G1期,逆转人成骨肉瘤细胞恶性表型特征,在促进终末分化标志物Ⅰ型胶原纤维蛋白、骨钙蛋白、骨粘素的表达同时,促进骨结节生成,从而说明HMBA能够诱导人成骨肉瘤MG-63细胞终末分化,并且Rg1、BPO-L、Rg1+BPO-L以及Rg1+HMBA等均具有终末诱导分化能力。
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DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.
诱导出的DC共同表达CD83和CD14分子,CD83分子表达明显高于国内报道;肝癌患者DC培养上清中TNF水平高于健康人,提示肝癌患者DC仍具一定的抗原呈递能力,适当调控可使其行使APC功能,以期在肝癌个体化疫苗中发挥作用;DC负载肝癌可溶性抗原后,抗原呈递能力降低,BCG HSP70可促进DC成熟,增加其抗原呈递能力,预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子;肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞,活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主,同时分泌较高水平Th1型细胞因子IL-12和TNF,并抑制4种肝癌细胞生长;冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤,提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用;用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽,在体外诱导肝癌特异性淋巴细胞,活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF,并具较强杀伤活性,此CTL同时具备NK细胞特征;DC对肿瘤细胞的抑制作用可能是通过吞噬实现的,Fas-L在DC抑制中也起一定作用;DC对人肝癌裸鼠皮下移植瘤的抑制率为97%;在肝癌DC疫苗的作用中,至少联合3种以上机制,即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。
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Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.
结果:酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间,给药剂量为80μg/kg/d时疗效最显著,达到69.24%,在20μg/kg/d-80μg/kg/d剂量范围内生命延长率为50-70%,给药剂量与荷瘤鼠生存时间呈现一定量效关系;酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长,给药剂量为160μg/kg/d时疗效最显著,抑制率为44.03%,并且在40-320μg/kg/d剂量范围内抑制率为25-50%,给药剂量与肿瘤抑制率呈现一定量效关系;酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用,在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显,其中浓度为100μg/ml时抑制率达19.36%;酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤:体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强,与效应细胞对照组相比有显著性差异(P<0.05)杀伤率分别达到35.58%、61.2%;体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强,与生理盐水对照组相比有显著性差异(P 。05),杀伤率分别达到21.39%、47.63%;裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强,与生理盐水对照组相比有显著性差异(P.05),最高杀伤率分别达到32.86%、73.07%;酪丝亮肤能增强单核巨噬细胞系统的吞噬功能,吞噬指数与生理盐水组比较有显著性差异(P.05);酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO,与效应细胞对照组相比有显著性差异(P.05);酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO,与生理盐水对照组相比有显著性差异(P.05);酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO,IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰,酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能,而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。
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Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.
研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。
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The recombinant TFPI-2 inhibits the invasive ability of human ovarian tumor cells in vitro, but has no effect on the migration, Which may provide a target basis for treating human ovarian tumor with TFPI-2 protein therapy.
重组TFPI-2对人卵巢癌细胞体外自身运动能力无影响,但可显著抑制人卵巢癌细胞的体外浸润能力,为将来卵巢癌用蛋白酶抑制剂治疗提供一可能的靶向依据。
- 更多网络解释与抑制人相关的网络解释 [注:此内容来源于网络,仅供参考]
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chalone:抑制素
challenge suggestion 查问暗示 | chalone 抑制素 | chameleon 反复无常的人
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containment:抑制
"颠覆"(subversion)和"抑制"(containment)是其文学社会功能论的两个非常重要的概念,"协合"就是在颠覆与抑制两种社会功能的动态关系中得以实现. 从一定意义上说,"颠覆"实际上是对人的社会性的颠覆,而"抑制"则是对人的自然性的抑制.
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Dam up sth:抑制某事
Dally with sth. 不认真考虑某事 * | Dam up sth. 抑制某事 * | Hail-fellow-well-met with sb. 对人十分友好 *
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inhibited:抑制的
尤其是到了18世纪(当时的政府规模三、四百万人,其构成有官员、有衙役、有士兵,还有获得许可证的垄断商人,而这个政府要面对的是三、四亿人民),是一个"受到抑制的"(inhibited)的政治中心,无法将其组织化的能力扩展到有限的空间以外,
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inhibited:抑制
内倾人格特征是:抑制(inhibited)、羞怯(shy)、缄默(reserved)和严肃(sober). 有重要的一点需要认识到,只有在极端的情况下我们才能谈及人格类型. 事实上,荣格曾提出过一种称作外倾-内倾的双极人格尺度. 大多数人不是处于两极的一个极端或另一个极端之上,
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Human retinoblastoma tumor suppressor protein,pRB ELISA Kit:人视网膜母细胞瘤抑制蛋白
Human Chromogranin,CgA ELISA Kit 人嗜铬蛋... | Human retinoblastoma tumor suppressor protein,pRB ELISA Kit 人视网膜母细胞瘤抑制蛋白(pRB)ELISA Kit | Human maturation promoting factor,MPF ELISA Kit 人成熟...
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Human retinoblastoma tumor suppressor protein,pRB ELISA Kit:人视网膜母细胞瘤抑制蛋白(pRB)ELISA试剂盒
人肿瘤血管生长因子(TAF)ELISA试剂盒 Human tumor ... | 人视网膜母细胞瘤抑制蛋白(pRB)ELISA试剂盒 Human retinoblastoma tumor suppressor protein,pRB ELISA Kit | 人成熟促进因子(MPF)ELISA试剂盒 Human maturati...
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suppress:抑制
当你完成这一任务时,达索克.快刀会对你说:"非常感谢你帮助我们成功抑制(suppress)了鹰身人的漫延. "而对疱疹病毒的治疗也可以称之为"抑制"(suppression). 在电影>中,有一句非常有名的台词"我看到了死去的人". 同时,
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zero sum two person game:零和二人竞赛
零和二人竞赛 zero sum two-person game | 消零,零抑制 zero suppression | 零抑制编辑,消零编辑 zero suppression editing
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put a damper on , damper:的原意是"起抑制作用的因素",这里比喻"令人扫兴的人或事",[扫兴]
82. put all one's eggs in one basket [孤注一掷] | 83. put a damper on , damper 的原意是"起抑制作用的因素",这里比喻"令人扫兴的人或事",[扫兴] | 84. put around 把人推来推去,比喻随意使唤