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- 皮内成骨
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Results Osteoblasts from human bone marrow were successfully induced and differentiated; the cuttlebones were reshaped in cuttlebone hydroxyapatite for the first time; and some tissue engineering bones, such as the osteoblasts/...
结果 人骨髓细胞培养中诱导、分化出成骨细胞;首次将乌贼骨改性为乌贼骨羟基磷灰石;构建出成骨细胞/ CHA5 0 0 R、成骨细胞/乌贼骨羟基磷灰石、成骨细胞/聚羟基丁酸酯等组织工程骨。
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Ore-binding factor alpha 1 plays an essential role in bone formation and osteoblast differentiation and serves as a master gene regulating osteoblast-specific gene expression. The gene is expressed in cells of the osteoblast lineage only, and when expressed in non-skeletal cells, the cells assume many of the characteristics of an osteoblast.
bfal在骨形成和成骨细胞分化过程中起着重要的作用,是调控成骨细胞特异性基因表达的主控基因,该基因只在骨形成相关细胞中表达,如果使其在非成骨细胞中表达,该细胞将出现成骨细胞的特征。
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The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.
1、取人胚胎颅骨骨膜,采有用酶消化法和组织培养法获取成骨细胞体外培养并传代,观察细胞形态,生物特点及成骨特性,并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径,将不同浓度的钛合金颗粒(1mg/ml,0.1mg/ml,0.01mg/ml)与成骨细胞共同培养,分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml,0.1mg/ml,0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。
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The cells in the cambial layer of the periostlum showed low or negative signal in the immediate injury response period. The osteoblasts differentiated from the periosteum cells stained strongly in the intramembranous ossification period, and the differentiated chondrocytes stained most strongly in the chondrogenesis period.
结果 在急性损伤反应期,血肿延伸范围内的骨膜内层细胞有少量表达;在膜内成骨期,成骨细胞表达量较高,骨膜细胞表达量少;在软骨形成期,成熟软骨细胞表达量最高;在软骨内成骨期,肥大软骨细胞不表达,成骨细胞表达量较高。
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In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.
上述结果证实:SMC内固定可对骨折端施加主动的、持续的轴向应力刺激,形成沿长骨骨干轴向的成骨导向力值,配合环抱翼的把持作用,形成了区别于CP、IN的独特的稳定、有效的机械内环境,骨折端的相对稳定减少了不必要的损伤再刺激,同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展,加快了骨折愈合的修复与重建。
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Results In all the animals injected with TGF-β, condrogenesis and osteogenesis were found under the periosteum and both intramembranous and endochondral osteogenesis were observed. The effect of impure TGF-β on osteogenesis took place at an earlier phase than that of pure TGF-β.
结果 注射TGF-β组动物股骨骨膜下皆可见新生软骨和骨组织,存在膜内成骨和软骨内成骨两种成骨方式,TGF-β粗提品组在成骨过程的时相上早于TGF-β纯品组。
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Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.
结果:(1)CBC具有天然的多孔网状结构,最大程度地保持了骨矿的微结构特征,可以作为骨组织工程的支架材料;(2)CBC细胞亲和性好,不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动;(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显,兼有膜内成骨和软骨内成骨的特点,成骨效果明显优于单纯CBC植入组;(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显,血管化充分,成骨效果明显优于单纯CBC植入组;(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好,明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组,可以作为骨移植替代材料用于骨缺损畸形修复。
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The osteogenous ability on the surface of UMCHA: MC3T3-E1 osteoblast was cultured on the surface of UMCHA to observe early stage of adhesion and proliferation, the osteogenous ability and configuration, BCBC and blank groups being controls.
UMCHA表面培养成骨细胞的活性:在UMCHA表面培养MC3T3-E1成骨细胞,并以空白和BCBC作为对照,观察成骨细胞的粘附率、增殖率、成骨活性和细胞形态。
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Effects of sinusoidally varying magnetic fields with one frequency of 2HZ and static magnetic fields with different strength of 10GS、200GS and 560GS on proliferation of osteoblasts had been observed. It had been found that PEMFs promoted proliferation of osteoblasts, static magnetic fields with intensities of 10GS and 200GS did not affect its proliferation whereas that of 560GS inhibited the proliferation.
通过观察频率为2HZ的正弦波电磁场及强度分别为10GS、200GS、560GS的静磁场对成骨细胞增殖的影响,结果显示:脉冲电磁场能显著促进成骨细胞的增殖;强度为10GS、200GS的静磁场对成骨细胞的增殖无明显影响,而强度为560GS的静磁场抑制成骨细胞的增殖。
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The tibiae of the rats were kept for the samples of decalcification, IGF-1 in bone tissue was measured by immunohistochemistry and Enzyme linked-immuno-sorbent assay was used to detect the TGF- P 1, IL-6, IL-1 P and TNF- a in serum.
结果 成骨细胞呈线状排列于骨小梁的边缘,细胞因子IGF-1表达于成骨细胞胞浆,骨细胞、软骨细胞及骨基质中也存在IGF-1。不同部位的成骨细胞IGF-1的表达量并不一致,卵巢切除及PTH注射对不同部位成骨细胞IGF-1表达的影响也不一样。
- 更多网络解释与成骨相关的网络解释 [注:此内容来源于网络,仅供参考]
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ossification:骨化,成骨
osseoalbuminoid 骨硬蛋白 | ossification 骨化,成骨 | osteoblast 成骨细胞
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ossification:成骨作用
ossification center 骨化中心 | ossification 成骨作用 | osteoblast 成骨细胞
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osteogenesis:骨发生 骨生成 成骨
osteofibrosis 骨纤维变性 | osteogenesis 骨发生 骨生成 成骨 | osteogenesisfamilialisimperfecta 家族性成骨不全
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distraction osteogenesis:牵张成骨
牵张成骨(distraction osteogenesis)最早应用于延长四肢的矫形外科,80年代末,这一技术逐步应用于各种先天性颌面发育不良和后天颌面缺损的治疗,疗效显著,但疗程一般较长,患者必须长期忍受牵张成骨所带来的诸多不便.
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chondrification center:软骨成骨中心
软骨粘液样纤维瘤 chondromyxoid fibroma | 软骨成骨中心 chondrification center | 软骨内成骨 endochondral ossification
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endochondral ossification:软骨内成骨
骨小梁形成.成骨细胞在骨小梁表面不断增长加粗(图4-12),逐渐形成为松质骨.松质骨的外侧部分逐步改建为密质骨,成骨区周围的结缔组织相应地转变为骨膜.2,软骨内成骨(endochondral ossification)软骨内成骨是在软骨雏珙发育的基础上逐步形成,
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intramembranous ossification:膜内成骨
并为骨的生长和修复提供成骨细胞.骨膜中的骨祖细胞具有成骨和成软骨的双重潜能,临床上利用骨膜移植治疗骨和软骨缺损等疾病.1,膜内成骨(intramembranous ossification)膜内成骨是在骨膜原始的结缔组织内直接成骨.少数骨以此方式发生,
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osteoblastoma:成骨细胞瘤
成骨细胞瘤(osteoblastoma)较骨样骨瘤更为少见. 其特点是在肿瘤内有较多的骨母细胞,血管丰富,可形成骨样组织和原始骨组织. 其同骨样骨瘤的关系密切,从显微镜下观察,两者病灶十分相似,唯骨母细胞瘤较大,一般直径大于2cm,X线片上骨膜反应不明显,
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osteoblastoma:成骨细胞瘤 骨母细胞瘤
osteoblastoclastoma 破成骨细胞瘤 | osteoblastoma 成骨细胞瘤 骨母细胞瘤 | osteoblasts 格根包尔氏细胞
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osteogenetic process:成骨过程
osteoepiphyseal portion 骨骺部 | osteogenetic process 成骨过程 | osteogenic action 成骨作用