- 更多网络例句与成纤维细胞的相关的网络例句 [注:此内容来源于网络,仅供参考]
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4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.
结果:(1)在无血清条件下单独培养的DRG,背根神经节的轴突数目众多,外形纤细弯曲,不成束,并且施万细胞和成纤维细胞稀少,所以可以排除两者对轴突生长的影响,为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件;(2)在无血清条件下DRG和变性坐骨神经段联合培养,①先单独培养DRG,4天后待神经元轴突长出,再与坐骨神经段联合培养,观察到神经元的轴突数目减少,外形挺直,部分轴突之间相互粘附成束;②变性坐骨神经段和DRG同时联合培养,神经元的轴突数目明显减少,外形粗壮,轴突之间相互粘附成束;(3)有血清条件下单独培养DRG,轴突数目较多,外形挺直,长短不一,部分神经元的轴突之间相互粘附,施万细胞和成纤维细胞数目众多,观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。
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Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.
以小鼠成纤维细胞株(NIH3T3)为研究对象,采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响,采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。
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In order to investigate the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scartricial fibroblast, the hypertrophic scar tissue of chest was chosen for culture of fibroblasts, and the influence of SM and TP on fibroblasts was observed, The effect of the drugs on the growth of fibroblasts, on DNA synthesis of fibroblasts and on mitosis index of fibroblasts were all determined quantitatively.
摘 要 增生性瘢痕是临床治疗难题之一,目前尚无有效的治疗方法。为了探讨丹参和川芎嗪对瘢痕成纤维细胞的抑制作用,取12例患者胸部增生性瘢痕组织,通过体外成纤维细胞培养,探讨不同药物浓度、不同作用时间对成纤维细胞生长的影响。
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The alveolitis that produces fibroblast proliferation and collagen deposition is progressive over time.
肺间质发生成纤维细胞的增生,成纤维细胞可以分泌大量的胶原纤维,最终导致了肺间质纤维化。
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When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.
当用电融合法进行核移植时,成体耳部成纤维细胞的融合率(46.0%),卵裂率(53.9%)和囊胚发育率(10.9%)均显著低于胎儿成纤维细胞(64.5%,70.1%和21.6%),胎儿皮肤细胞(71.5%,70.8%和22.1%),以及卵巢颗粒细胞(88.2%,79.1%和25.5%);另外三种细胞间的卵裂率,囊胚发育率无显著差异,但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞(88.2%vs 64.4%,71.5%,P<0.05)。
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Rat dermal fibrobalasts and human neofetus dermal fibroblasts were cultured in vitro to evaluate the biocompatibility of the chitosan films.
为考察壳聚糖对皮肤成纤维细胞的相容性,采用流延法制备了纯壳聚糖膜和含甘油的壳聚糖膜,并在所制备的壳聚糖膜上进行了大鼠皮肤成纤维细胞和人胎儿皮肤成纤维细胞的体外培养。
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Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.
应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养,细胞铺满培养瓶底后首次传代,通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化;通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况,对细胞进行鉴定。
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Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.
中文题名尿道瘢痕基础研究副题名胶原酶活性,TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase,expression of TIMP-1,and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的:研究尿道瘢痕的细胞外基质的组成,尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响;比较胶原酶活性,金属蛋白酶组织抑制因子-1(TIMP-1)以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异;研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。
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Methods: Electrets Teflon PTFE,±300 V,±1 000 V were used to treat 3T3 cells for 24, 48 and 72 h. Then the influences of electrets on cell cycle and surface charge of 3T3 cells were studied by flow cytometry and electrophoresis, respectively.
选用±300 V和±1 000 V驻极体分别作用成纤维细胞24、48和72 h,用流式细胞术和细胞电泳技术测定成纤维细胞的生长周期和细胞表面电荷。
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Results: Compared with control group, apoptosis cells increased from 0.5%to 10%(some even to 15%) after 24,48 and 72 h action of -300,-500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles.
结果:-300、-500和-1 000 V驻极体作用成纤维细胞24、48和72 h以后,与对照组相比,成纤维细胞的凋亡量从0.5%增至10%(部分可达15%);驻极体作用成纤维细胞48~72 h,出现细胞凋亡特有的形态学特征,即:细胞异染色质边集,细胞裂解,可见凋亡小体。
- 更多网络解释与成纤维细胞的相关的网络解释 [注:此内容来源于网络,仅供参考]
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fibroblast:成纤维细胞
1 成纤维细胞的来源及其生物学特性 成纤维细胞(fibroblast)是结缔组织中最常见的细胞,由胚胎时期的间充质细胞(mesenchymal cell)分化而来. 在结缔组织中,成纤维细胞还以其成熟状态-纤维细胞(fibrocyte)......
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cell senescence:细胞衰老
与细胞增殖、分化、凋亡一样,细胞衰老(cell senescence)也是细胞的基本活动之一. 细胞衰老现象最初为Hayflick在正常成纤维细胞的体外培养中观察到,发现正常细胞在体外条件下增殖分裂50~70代即进入一种衰老的状态,细胞丧失继续增殖的能力无法进一步传代培养,
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desmocyte:皮纤维细胞
desmocranium 颅胚(中胚层的) | desmocyte 皮纤维细胞 | desmocytoma 成纤维细胞瘤
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fibrinous:含纤维(素)的;由纤维素形成的
fibrinoplastin 副球蛋白 | fibrinous 含纤维(素)的;由纤维素形成的 | fibroblast 成纤维细胞
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fibroblastic:成纤维细胞的
fibroblastic 纤状变晶状 | fibroblastic 成纤维细胞的 | fibrocaseous 纤维干酪性的
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fibroblastic:成纤维细胞的/纤状变晶状
fibroblast /纤维母细胞/成纤维细胞/ | fibroblastic /成纤维细胞的/纤状变晶状/ | fibroblastoma /成纤维细胞瘤/
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fibrocaseous:纤维干酪性的
fibroblastic 成纤维细胞的 | fibrocaseous 纤维干酪性的 | fibrochondromainochondroma 纤维软骨瘤
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mesenchymal cell:间(充)质细胞
是结缔组织中最常见的细胞,由胚胎时期的间充质细胞(mesenchymal cell)分化而来. 在结缔组织中,成纤维细胞还以其成熟状态-纤维细胞(fibrocyte)的形式存在,二者在一定条件下可以互相转变. 不同类型的结缔组织含成纤维细胞的数量不同. 通常,
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fibroblasts:成纤维细胞
成纤维细胞(Fibroblasts) 成纤维细胞是人体中广泛存在的重要的结缔组织细胞,正常情况下,真皮结缔组织中只有散在分布的少量成纤维细胞,它们处于代谢和功能的静息状态,只有在创伤或某些疾病过程中才可能活跃地显现出其生物学特性,
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cardiac fibroblasts:心脏成纤维细胞
心脏成纤维细胞(cardiac fibroblasts)以其细胞数量而言,在心脏中是一个庞大的细胞群体.它们形成了心肌层的结构、生物化学、机械-电等方面的特性.即便如此,它们却经常在心脏功能的研究中被人们所忽略.本综述扼要阐述了成纤维细胞起源及鉴定,