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Methods Adult mosquitoes were captured by mosquito?net trap, light trap and mosq?
成蚊调查采用帐诱法、诱蚊灯法和诱蚊诱卵器法。
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When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.
当用电融合法进行核移植时,成体耳部成纤维细胞的融合率(46.0%),卵裂率(53.9%)和囊胚发育率(10.9%)均显著低于胎儿成纤维细胞(64.5%,70.1%和21.6%),胎儿皮肤细胞(71.5%,70.8%和22.1%),以及卵巢颗粒细胞(88.2%,79.1%和25.5%);另外三种细胞间的卵裂率,囊胚发育率无显著差异,但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞(88.2%vs 64.4%,71.5%,P<0.05)。
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In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV. The results showed that WSSV positive cases were detected in copepoda from egg to adult, and the positive copepoda appeared earlier than cultural shrimp in ponds.
1。本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵,以及由这些休眠卵孵化出的幼体携带病毒情况,发现桡足类由卵到成体各阶段中均有阳性个体,而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。
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Methods About 142 pairs of monozygotic twins and 86 pairs of dizygotic twins aged 5-19 were studied.
选取 5~ 1 9岁双生子 2 2 8对,其中MZ 1 4 2对,DZ 86对,在DNA卵性鉴定基础上,以成对法计算同病一致率、调整BMI前后组内相关系数及遗传度。