- 更多网络例句与带氧酶相关的网络例句 [注:此内容来源于网络,仅供参考]
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Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.
从自然感染的病羊脑内采集多头蚴原头节,提取总RNA,采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段,PCR产物连接到pMD18-T载体,转化至大肠杆菌DH5α后测序,发现克隆到的TmTPx基因片段开放阅读框为453bp,编码150个氨基酸。
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RESULTS: Image analysis of two-dimensional map revealed 72 differentially expressed proteins between MHCC97-H and MHCC97-L cells, and 9 protein peaks (including M2 pyruvate kinase,α-subunit of ATP synthase, heat shock protein 60, Toll-like receptor 9, flavoprotein oxygenase, pro-calreticulin, manganese superoxide dismutase, nm23-H1, and G-protein-coupled receptor kinase 5) were further identified by tryptic digestion, peptide mass fingerprinting and mass spectrometry.
结果: 2D图谱显示共有72个差异蛋白条带,共鉴定出了9个差异蛋白,分别为M2型丙酮酸激酶、ATP合成酶α亚单位、热休克蛋白60、Toll样受体9、含黄素单加氧酶、钙网硬蛋白前体、锰超氧化物岐化酶、nm23-H1、G-蛋白偶连受体激酶5;其中4个蛋白在高转移细胞株MHCC97-H中表达升高, 5个蛋白在低转移细胞株MHCC97-L表达升高。