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Nov.. The main symptoms of the bacterial disease on Vigna angularis were canker of stalk.The strains were straight or curved rods; polar flagellum one; Gram-negative; could infect Vigna angularis、 Glycine max、 Vigna radata、 Vigna umbellate、 Phaseolus vulgaris、 Pisum sativum、 Pisum sativum var.humlie; oxidase negative; nitrate reduction negative; 5% glucose agar mucilage positive; esculin hydrolysis positive; starch negative; milk hydrolysis positive; H_2S positive; acid from L-arabinose, D-mannose, D-galactose, cellobiose, trehalose and fructose; 5%NaCl positive; G+Cmol%-69%.
红小豆溃疡病主要症状为茎部溃疡；各菌株菌体均杆状；极生鞭毛1根；革兰氏染色阴性；人工接种能侵染多种豆科植物；好氧；氧化酶阴性；硝酸盐反应阴性；5％葡萄糖营养琼脂黏液生长阳性；七叶灵水解阳性；淀粉水解阴性；牛奶分解产碱；产生硫化氢；能从阿拉伯糖等产酸；最大耐盐量为5％；G+Cmol％为69％等特征，根据《手册》9th ed相关部分，确定该病由野油菜黄单胞菌苜蓿致病变种Xanthomonas campestris pv.alfalfae(Riker，Jones et Davis 1935)Dye 19781引起。
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TLC and HPLC proved that emodin and rhein were existed in the hairy root with free and combinative forms.
TLC和HPLC证明何首乌毛状根中含有大黄索和大黄酸成分且均以游离和结合两种形式存在。
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Results: The hairy root was successfully induced and the hairy root culture system was set up; The opines assay was positive for mannopine and agropine; The result of determination showed that there were a lot of anthroquinone constituents in the rhubarb hairy root and the contents of rhein, aloe-emodin, emodin or 8-O-methylchrysophanol were respectively higher than those in wild plant root.
结果：诱导出了天山大黄发根，并建立了发根离体培养系；从发根中检测到甘露碱、农杆碱的存在；定量分析发现，天山大黄发根中含有多种蒽醌类成分，其中大黄酸、芦荟大黄素、大黄素、大黄酚-8-甲醚的含量分别高于原植物根中的含量。
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Methods: The medicinal plant Rheum wittrochii Lundstr., was genetically with Ri plasmid harbouring in Agrobacterium rhizogenes 9402 strain to induce hairy roots; transformed the opines from the hairy roots were assayed by paper electrophoresis; the contents of free anthroquinones such as aloe-emodin, rhein, chrysophanol, emodin, physcion, 8-O-methylchrysophanol from the hairy roots were quantitatively determined by HPLC technique.
用发根农杆菌Agrobacterium rhizogenes 9402 所含Ri质粒遗传转化药用植物天山大黄以诱导发根用纸电泳法检测发根中冠瘿碱的存在；用高效液相色谱法测定发根中芦荟大黄素、大黄酸、大黄酚、大黄素、大黄素甲醚、大黄酚-8-甲醚等游离蒽醌化合物的含量。
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Aloe-emodin and rhein were predominant in five anthraquinones after being treated with rare-earth element Eu(superscript 3+).
经Eu(上标 3+)处理的3种类型大黄根中芦荟大黄素和大黄酸高于大黄素、大黄酚和大黄素甲醚。
- 更多网络解释与大黄根酸相关的网络解释 [注：此内容来源于网络，仅供参考]
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alizarin：茜草素
[化学成分]根含多种羟基蒽醌衍生物,如茜草素(alizarin)异茜草素(purpuro-xanthin)羟基茜草素(purpurin)伪羟基茜草素(pseudopurpurin)茜草酸(munjistin)茜草甙(rubia,ruberythric acid)大黄素甲醚等,又分离得升白活性成分茜草萘酸甙Ⅰ及Ⅱ,
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Purpurin：羟基茜草素
[化学成分]根含多种羟基蒽醌衍生物,如茜草素(alizarin)异茜草素(purpuro-xanthin)羟基茜草素(purpurin)伪羟基茜草素(pseudopurpurin)茜草酸(munjistin)茜草甙(rubia,ruberythric acid)大黄素甲醚等,又分离得升白活性成分茜草萘酸甙Ⅰ及Ⅱ,
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munjistin：茜草酸
[化学成分]根含多种羟基蒽醌衍生物,如茜草素(alizarin)异茜草素(purpuro-xanthin)羟基茜草素(purpurin)伪羟基茜草素(pseudopurpurin)茜草酸(munjistin)茜草甙(rubia,ruberythric acid)大黄素甲醚等,又分离得升白活性成分茜草萘酸甙Ⅰ及Ⅱ,
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Protocatechuic acid：原儿茶酸
亦含鞣质和多糖.从根中分离出的酚性成分有: 迷人醇(Fallacinol)、6-羟基芦桧大黄素(Citreorosein)、大黄素-8-单甲醚(Questin)、6-羟基芦桧大黄素-8-单甲醚(Questinol)、原儿茶酸(Protocatechuic acid)等.还分离鉴定了槲皮素(Quercetin)、槲皮素-