- 更多网络例句与大的厚皮动物相关的网络例句 [注:此内容来源于网络,仅供参考]
-
And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.
1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠,雌雄不限(中国医科大学实验动物中心提供),腹腔注射10%水合氯醛0.4ml/100g,无菌条件下取出气管,无菌PBS反复冲洗,洗净血液和粘液,置于DMEM/F12培养液中(含10%胎牛血清),于培养液中加入终浓度为100mg/ml的5-Fu,37℃,5%CO2孵育12小时,弃去上述培养液,换成新鲜DMEM/F12液(含10%胎牛血清),同时加入终浓度为200nM的TSA继续培养,于换液后3、6、12、24、48小时分别10%中性福尔马林固定,石蜡包埋,制成4μm厚的组织切片。
- 更多网络解释与大的厚皮动物相关的网络解释 [注:此内容来源于网络,仅供参考]
-
Dumbo:小飞象
他以更平凡的风格提供给我们"小飞象"(Dumbo),一个关于有着一对大耳朵的厚皮动物之感伤的杂记英国电影以莱斯利.霍华德(Leslie Howard)表演的两个故事表现了专制和自由之间的鸿沟,他成为朴素的英国理想的象征.