复性

基本解释 (translations)

renature

与复性相关的网络例句 [注：此内容来源于网络，仅供参考]

It was founded that the renaturation yield was improved in the presence of arginine, thiourea, acetone, acetamide and glycerol compared with no additives.

分析其作用机制异同点；并基于海藻糖和甜菜碱对保护蛋白质的作用，考察了海藻糖和甜菜碱在不同浓度盐酸胍和酶浓度条件下对溶菌酶辅助复性和复性动力学特性，分析了它们作用机理。

This chapter is divided into three sections, section I, for the analysis of "Lao Zi" annotator in Qing Dynasty in the impact of three religions, it was agreed that "eliminate emotion and regress to humanity" idea. Section II to illustrate mainly the traditional "Lao Zi" annotation in Qing Dynasty, the impact in Western countries began to accept the idea of Western Ideas, output the first "Lao Zi" annotation base on Western Ideas –Yan Fu "Ping Dian Lao Zi"; Section III of "freedom, democracy, equality", to discuss Yan Fu think "Lao Zi" already have many of the modern social values:"free","equality","teamwork".

本章共分为三节，第一节为「复性化情」，析论清代《老子》注家在三教思想的濡染下，一致提出「复性化情」的主张，作为体道、得道的路径；第二节为「会通之道」，说明以传统守成态度为主的清代《老子》注，在西方国家的冲击下，开始接纳西学的理念，开出了第一本以西学解老的著作──严复《评点老子》，此书反映了他中西学术兼容并蓄的思想，他不仅融合儒、释、道的概念，更将西方的科学、哲学纳入《老子》的思考当中；第三节为「自由、民主、平等」，旨在讨论严复认为老子书中已经具备许多近现代的社会价值：「自由」、「平等」「合群」的概念。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

The PCR amplification process was 1 cycle initial denaturalization at 94 ℃ for 5 min, followed by 35 cycles, which included denaturalization at 94 ℃ for 30 s, annealing for 1min, extend at 72 ℃ for 2 min.

PCR扩增程序为：94 ℃预变性5 min；94 ℃变性1 min，35 ℃复性1 min，72 ℃延伸90 s，5个循环；94 ℃变性1 min，50 ℃复性1 min，72 ℃延伸90 s，35个循环；72 ℃延伸8 min.4 ℃保存。

The role of arginine to assist protein refolding was similar to that of acetone, both of them were able to combine with the denature lysozyme and improve the refolding yield.

结果表明，与自发复性收率(40%)相比，复性液中添加精氨酸、甘油、乙酰胺、丙酮、硫脲、海藻糖和甜菜碱均能有效提高变性溶菌酶的复性收率。

The recombinant proteins form inclusionbody in cytoplasm. Dissolve the inclusionbody with urea and refold it with metal affinity chromatography. The refold recombinant protein has certain antibacterial activity.

对表达产物进行复性，结果显示无论是融合表达的VHb-Fetidin还是非融合表达的Fetidin都无法用稀释法和尿素浓度梯度透析法成功复性，只有VHb-Fetidin可以通过金属亲和层析柱成功复性。

The effect of guanidine hydrochloride concentration on the oxidative refolding of denatured-reduced lysozyme of high concentration in the presence of artificial chaperone , that is, cetyl trimethyl ammonium bromide and β-cyclodextrin,was studied.

研究了表面活性剂十六烷基三甲基溴化铵加β-环糊精和盐酸胍对高浓度变性-还原溶菌酶氧化复性的影响，利用基于二态复性模型的表观动力学模型分析了溶菌酶的复性过程。

After collecting the bacterial cells and breaking the cell by means of ultrasonic, the inclusion bodieswere extracted. The extracted products were denatured with 8 mol/L urea and purified through Ni-NTAcolumn. The purified products were renatured by dialyzation, and then were analyzed by SDS-PAGE.

在优化条件下诱导重组菌表达，收集菌体经超声波破碎和裂解后提取不溶性包涵体，以8mol／L尿素处理包涵体提取物并进行Ni-NTA亲和层析纯化，纯化表达产物经透析复性后以SDS-PAGE分析其复性效果。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

The purity of the refolded rhIFN-γ was up to 90%, and high urea gradient length and moderate final urea concentration were favorable for the refolding process. Under optimum conditions, the specific activity of rhIFN-γ was up to 9.75×10^6IU/mg and protein recovery was up to 53.7%.

复性后的重组人γ-干扰素的纯度达90%；较长的脲梯度长度和合适的脲终浓度有利于蛋白质的复性；在较优的实验条件下，复性后的重组人γ-干扰素收率为53.7%，比活达9.75×10^6IU/mg。

与复性相关的网络解释 [注：此内容来源于网络，仅供参考]

heterogeneity：不均一性

(三)DNA结构的不均一性(heterogeneity)1.DNA变性(denaturation)2.DNA复性(renaturation)不同来源的核酸变性后,合并在一处进行复性,这时,只要这些核酸分子的核苷酸序列含有可以形成碱基互补配对的片段,复性也会发生于不同来源的核酸链之间,

plural society：多元社会,复性社会

Pleasure Principle 快乐原则530 | Plural Society 多元社会,复性社会156 | Poetique 诗论,诗学820

redox potential：氧化还原电位

蛋白复性是一个复杂的过程,诸如pH、离子强度、低分子添加剂、氧化还原电位(redox potential)和温度等物化因子,影响体外蛋白复性的效率. 通常体外复性的方法包括稀释法和缓冲交换法. 前者通过化学处理,

compound hyperopic astigmatism：复性远视散光

compound astigmatism 复性散光 | compound hyperopic astigmatism 复性远视散光 | compound myopic astigmatism 复性近视散光

involatile substance：不挥发性物质

diplomellituria 复性糖尿, 双糖尿 | involatile substance 不挥发性物质 | transfer pump 传送泵, 输送泵

polygenic：多基因性

(三)HLA 复合体的遗传特征(多基因性,多态性,单元型遗传,共显性遗传,连 锁蒸平衡) 多基因性(polygenic)即在同一个体中,HLA 复合体基因座位在数量 和结构上具有多样性;多态性(polymorphism)是指在一随机婚配的群体中,

renature：复性

适当调整温度或pH值,分开的两条互补链又可通过碱基配对重新形成双螺旋,这一过程称为复性(renature)或退火(annealing )(图2-8).DNA变性和复性的特点是分子生物学重要技术核酸分子杂交的基础.寡糖链(主要是其中的中性糖,

On-column refolding：柱内复性

推进剂:solid propulsive agent column | 柱内复性:On-column refolding | 柱色谱分离:column chromatography

Dilution refolding：稀释复性

反淡化理论:Dilution | 稀释复性:Dilution refolding | 淡化行为:The dilution behavior

in vitro refolding：体外复性

体外获能:in vitro capaicitation | 体外复性:in vitro refolding | 冷冻:isolation and culturation in vitro