基因文库

词组短语

gene bank · gene library

与基因文库相关的网络例句 [注：此内容来源于网络，仅供参考]

TRP1 gene of Candida glycerinogenes (CgTRP1), encoding phosphoribosyl anthranilate isomerase(PRA1) was cloned by complementation of the trp1 mutation of Saccharomyces cerevisiae W303-1A.

本文采用遗传互补的方法从产甘油假丝酵母基因文库中克隆了TRP1基因(CgTRP1)。

MRNAs were obtained from 7 stages of appressorium formation in Magnaporthe grisea. Through SSH strategy, a 250 unique ESTs library was construted, among which 177 sequences were loaded in the gene bank.

从稻瘟病菌7个附着胞形成时期分离了mRNAs，通过SSH的方法获得了该病菌成熟附着胞（24小时）的独有的ESTs序列250条，其中基因文库中登录177条。

Carcinoma ; hepatocellular ; Gene library ; Augmenter of liver regeneration ; Interacting protein

癌； 肝细胞；基因文库；肝再生增强因子；相互作用蛋白

Then the recombinant plasmid transformed E.coli.JM109. One positive clone was obtained from the libraries with casein flat and ELISA sieve method.

用酪蛋白平板法和酶联免疫吸附的活性筛选方法从基因文库中筛选到一株产海洋低温碱性酶的阳性克隆（命名为pHH1）。

Result:The subtractive cDNA libraries related with apoptosis of NB4 cells were successfully constructed.

结果： 成功构建了分别代表在NB4细胞中表达上调和下调的基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis ×L. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

Arteries ; Heart ; Gene library ; Expressed sequence tags ; DNA ; comple mentary

动脉；心脏；基因文库；表达序列标签； DNA ；互补

It was reported that the pHZ1392 isolated from a genomic cosmid library of 10-22 might contain the complete gene cluster for 5102-Ⅱ bio-synthesis, which can inhibit some pathogenetic fungi, such as Cochliobolus heterostrophus and Piricularia oryzae, etc.

在过去的研究中曾报道10-22基因文库中的一个粘粒-pHZ1392可能含有完整的5102-Ⅱ抗生素合成基因簇，如果以pHZ1392转化变铅青链霉菌ZX64，可以使该宿主菌获得对玉米小斑病菌的抗性。

与基因文库相关的网络解释 [注：此内容来源于网络，仅供参考]

Bias：偏性

但由于"cDNA计划"当初是为鉴定新基因而设计,鉴于文库组成的"偏性"(bias),由该方法收集到的EST并不能作为特定细胞或组织表达基因的量化分析的数据来源.

positional cloning：位置克隆

功能性基因组计划的根本目标是发现占基因组3%左右有表达功能的区域,为此将构建大片段DNA文库作位置克隆(positional cloning)和作大规模的表达序列目标(ESTs)分析,以期经济快捷地抢占基因资源.

DNA sequencing：测序

[摘要]分子诊断技术在遗传性疾病、肿瘤和感染性疾病的诊断领域内占有重要的位置,DNA测序(DNA sequencing)为其中最精确的一种方法,主要用于鉴定基因,检测突变,分析噬菌体文库等方面.

gene transfer：基因导入,转基因

gene library 基因文库 | gene transfer 基因导入,转基因 | genetic code 遗传密码

subtracted library：扣除文库

差示文库(differential library)或扣除文库(subtracted library), 是反映不同组织细胞或同一种细胞在不同功能状态下基因表达差异的cDNA文库. 用于研究细胞的发育、分化、细胞的分裂周期、细胞对药物和生长因子的诱导反应以及肿瘤等疾病的分子基础.

Major Ampullate Gland：主壶腹腺

文章摘要:首次通过反转录-置换法和使用pUC18质粒成功构建大腹圆蛛(Araneus ventricosus)主壶腹腺(major ampullate gland)cDNA基因文库,并以鸟枪法从中筛选出具有典型重复结构的大腹圆蛛主壶腹丝蛋白cDNA基因AvF1,大小为1744bp,编码区为1572bp,

genomic library：基因组文库

7、基因组文库:(genomic library)整个生物体基 因组随机产生的重叠DNA片段的克隆的总和8、基因库:gene bank 某一生物群体中全部个体 所共有的全部基因 9、蛋白质组:proteome 在某种细胞或组织中整个 基因组所产生的各种蛋白质的组合.由基 因组编码的全部蛋白质总合 10、蛋白质组学:proteomics 研究一个基因组中基 因编码的

genomic library：基因文库

的筛选和鉴定 目的基因(target DNA)的制备 目的基因 目的基因(外源基因) 目的基因(外源基因) 制备基因组DNA 制备基因组 - 基因文库(genomic library)-存在于转化细菌 基因文库 存在于转化细菌 克隆载体携带的所有基因组DNA的集合中,

probes：探针

首先需要制备探针(probes). 探针是根据所需基因的核苷酸顺序制成一段与之互补的核苷酸短链,并用同位素标记. 下图就是用分子杂交的方法从基因文库中钓取目的基因. 图10-3-7 用分子杂交的方法从基因文库中钓取目的基因4、PCR扩增 在获取目的基因后,