培养液

词组短语

culture fluid · nutrient fluid

与培养液相关的网络例句 [注：此内容来源于网络，仅供参考]

An experiment was performed to compare effect of different cell media on embryonic germ cells derived from primordial germ ridge of Kunming albinotic strain mouse.

以昆明白品系小鼠胎儿生殖嵴为材料，以不同的培养液分离培养胚胎生殖细胞，发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子的基础培养液，而差于添加了1 000 IU/mL LIF的基础培养液。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group cnpared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early cnpared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越多的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越多的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

The tissue was dissociated by digestion and triturating and made into single cell suspension, which was cultured in DMEM/F12 media containing 10% FBS, DMEM/F12 media containing 1% N2 supplement (N2 group), DMEM/F12 media containing 1%B27 supplement (B27 group) and DMEM/F12 media containing 1%N2 and 1% B27 supplements (N2+B27 group), the growth and purity of OECs were investigated.

方法分离新生2天SD大鼠的嗅球，制成单细胞悬液，在含10％FBS的DMEM／F12培养液、含1％N2的DMEM／F12培养液(N2组)、含1％B27的DMEM／F12培养液(B27组)以及含1％N2和1％B27的DMEM／F12培养液(N2+B27组)中进行培养，观察OECs的生长状态。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

The results showed that the most suitable time to isolating the Larva from the experiment animals, which were experimentally infected with canine Trichinella was on the fiftith day; the most suitable time of degesting was at 37℃ for 17 hours; 38℃ for 14 hours; 39℃for 19 hours; 40℃ for 10 hours; the most suitable inquantity to inoculate per millilitre was 6000 strips; the best temperature was at 37℃; the best gas condition was in the CO2gas incubator and the concentration of CO2 was 8 per cent; the most suitable pH was at 7.0; the most suitable medium was RPMI-1640; the best nourishment component was peptone; the most appropriate time interval of changing medium was about 24 hours; the best time to observe was at 24 hours.

试验结果表明，从人工感染犬旋毛虫的实验动物中分离肌幼虫的最适日龄为50天，最适虫体消化时间为37℃时17h、38℃时14h、39℃时12h、40℃时10h，每ml培养液最适虫体接种量为6000条，最佳培养温度为37℃，最适培养气体条件为CO_2二氧化碳培养箱、最适培养液pH值为7.0，最适培养基为RPMI—1640，最佳培养液附加营养成分为蛋白胨，最佳培养液更换时间间隔为24h，最适观察虫体时间间隔为24h。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

与培养液相关的网络解释 [注：此内容来源于网络，仅供参考]

culture：培养

无菌组织块经培养液洗液洗涤后制成10~20%悬液离心后,取上清接种;咽洗液,粪便,尿,感染组织或昆虫等污染本在接种前先用抗生素处理,杀死杂菌.1.细胞培养用分散的活细胞培养称细胞培养(culture).所用培养液是含血清(通常为胎牛血清),

culture medium：培养液,培养基 培養基

culture 栽培,培养,培养物 培養; 養殖; 文化 Y | culture medium 培养液,培养基 培養基 Y | current annual uptake 当年吸收量 當年吸收量 Y

defined medium：确定成分培养基,已知成分培养液

defined 确定的 | defined medium 确定成分培养基,已知成分培养液 | defintion 定义

defined medium：确定成分培养基,己知成分培养液

defined 确定的 | defined medium 确定成分培养基,己知成分培养液 | definition 定义

feeding：饲养

24h后倾去培养皿内种植培养液,改用饲养(Feeding)培养液培养. 接种第3d, 在培养皿中分别加入细胞分裂抑制剂5-氟-2'-脱氧尿苷15μg/ml和尿苷35μg/ml, 作用48 h后更换新鲜饲养培养液,以后每周换液两次, 每次更换一半新鲜饲养培养液.

nutrient solution：培养液

nutrient 营养,培养基 | nutrient solution 培养液 | nutriment 营养,营养品

tank farming：培养液植物

tank engine 蒸气火车头 | tank farming 培养液植物 | tank ship 油船

Phytophthora capsici：辣椒疫霉

辣椒疫霉(Phytophthora capsici)是黑龙江省南瓜疫病的病原菌,其在培养液中培养时可分泌毒素,该毒素能引起南瓜叶片产生水渍状褐腐斑,及幼苗的萎蔫,类似病原菌侵染后产生的症状.Plich's培养液有利于P.capsici的生长和产毒,而胡萝卜汁培养液只是有利于其生长,

culture fluid：培养液

culture by rice hull 稻糠培育 | culture fluid 培养液 | culture in vitro 体外培养

protein-free medium：无蛋白培养基,无蛋白培养液

protein folding funnel 蛋白质折叠漏斗 | protein-free medium 无蛋白培养基,无蛋白培养液 | protein functional domain 蛋白质功能域