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The results show that this strain was a typical acidophile with optimal growth pH being 2.0 for hypha and pH 3.0-4.0 for the spore germination. Peptone was the most suitable nitrogen source which helped produce xylanase induced by xylan during stationary phase. The resultant xylanase reacted best at 50℃-55℃ and pH 3.5, and had activities 70% after 60 min maintenance under optimum reaction conditions.
结果表明:实验菌株嗜酸,菌丝生长最适pH为2.0,孢子萌发生长适宜pH为3.0-4.0;木聚糖诱导菌体在生长稳定期大量产生木聚糖酶,蛋白胨是菌体产酶的适宜氮源;菌株所产木聚糖酶属于酸性木聚糖酶,反应最适pH3.5、最适温度50℃-55℃,在最适反应条件下保温60min,残余酶活仍接近70%,适用于较强酸性的高温加工环境。
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The number of EOS in peripheral blood were counted by counting plate and the morphological changes in bronchia and EOS infiltration in lung tissue were observed. The content of NO and ET-1 in blood serum and BALF were measured by biochemistry and radioimmunity methods.
用计数板计数外周血嗜酸性细胞数量,观察小支气管形态学改变和肺组织中EOS浸润情况;生化法和放免法测定血清和肺泡灌洗液中一氧化氮和内皮素-1(ET-1)的含量。
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The highest enzyme producer alkalophilic Bacillus sp SHY8-1 among the inductive strains was mutagenized several times, by adding enzyme production repression aegents into screen medium, combined with natural seperation and pressure selection, finally a CMCase-hyperproductive mutant SHY8-1-25 (partitally resistant to catabolite) was obtained, with the yield of CMCase raised to 13-16u/ml, maximally reached 18-20u/ml, while its parent strain 0.654u/ml.
对诱导型产酶菌-嗜碱芽孢杆菌SHY8-1进行了多次诱变改良,采用添加产酶阻遏剂的平板作为筛子,结合反复的自然分离、压力选择,最终成功地获得了高产变异株SHY8-1-25(已部分解除分解代谢阻遏),产酶量由0.654u/ml提高到13-16u/ml,最高达到18-20u/ml,讨论了诱变机理策略,考察了碳、氮源等对产酶的影响,对培养基和摇瓶培养条件进行了优化。
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The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.
分别于缺血4h末,再灌注1、2、4、8、12h,测定各组血浆中的MDA、骨骼肌组织中的MPO,血清嗜中性粒细胞化学趋化因子(CINC/IL-8),血清一氧化氮,用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b/CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。
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After feeding of 20 d, 40 d and 60 d, the activities of nitric oxide synthase, bacteriolytic, superoxide dismutase, alkaline phosphatase and acid phosphatase in the serum were determined and the relativities between them were also analyzed.
在饲料中添加不同剂量的嗜酸小球菌投喂凡纳滨对虾,并在投喂后的20、40和60d,分别测定凡纳滨对虾血清中的一氧化氮合酶、溶菌活力、总超氧化物歧化酶、酸性磷酸酶、碱性磷酸酶,并分析了这5种酶之间的相关性。
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There was a plus relationship between NOS and bacteriolytic activity and a minus relationship between NOS and SOD as well as a minus relationship between bacteriolytic activity and AKP, which indicated that feeding Pediococcus acidilactici could significantly increase the activities of the immune factors in the shrimp body.
表明在饲料中添加适量的嗜酸小球菌(剂量10mg/kg)可使凡纳滨对虾体液免疫因子活力到达较高的水平;凡纳滨对虾血清中一氧化氮合酶对于嗜酸小球菌的添加量较为敏感,可作为评价嗜酸小球菌使用效果的指标之一。
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Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.
YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群,序列同源性大于99%,与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6,6~18,18~66和66 h;培养12 h后,YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56,此后逐渐上升,至发酵结束时其pH值分别为7.74和8.07;培养0~18 h时2菌株发酵液中还原糖含量迅速降低,此后保持稳定;12 h时氨基氮含量达到最低,此后开始缓慢上升;YL001菌株培养54 h后及YL002菌株培养42和66 h后,其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。
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85 And 119486/79. They are a novel type of antibiotic having both the wide antibacterial spectrum and high safety of penicillin and cephem antibiotics belonging to beta -lactam antibiotics, as well as the potent antibacterial activity and high beta -lactamase stability of carbapenem antibiotics.Sodium-(5R, 6S)-6-[-1-hydroxyethyl]-7-oxo-3- [-2-tetrahydrofuryl]-4-thia-1-azabicyclo [3.2.0] hept-2-ene-2-carboxylate 5/2 hydrate (faropenem sodium, hereinafter referred to as compound 1) is currently used as an oral drug for various infectious diseases and is reported to show potent antibacterial activity against not only methicillin-sensitive Staphylococcus aureus, Streptococcus pyrogenes and Streptococcus pneumoniae but also gram-positive bacteria for which conventional beta -lactam drugs have proved ineffective such as penicillin-resistant pneumococci, oral staphylococci and enterococci, also showing a wide antibacterial spectrum covering gram-negative bacteria such as Haemophilus influenzae and anaerobic bacteria such as the genus Bacteroides, which activity is due to its novel skeleton penem ring (Kagaku Ryoho no Ryoiki The Field of Chemotherapy, Vol.
他们是一种新型的抗生素都具有广泛的抗菌谱和高度的安全青霉素和cephem抗生素,属于β-内酰胺类抗生素,以及作为强大的抗菌活性和高β-内酰胺酶稳定的碳青霉烯类antibiotics.sodium -( 5 R , 6 S )-6 -[ - 1 -羟乙基] - 7 -氧- 3 - [ - 2 - t etrahydrofuryl] - 4 -硫杂- 1 -氮杂双环[ 3 。2.0]庚- 2 -节能- 2 -羧酸5 / 2水合物(法罗培南钠,以下简称为化合物1 )是目前用来作为口服药物,为各种传染病和报道,以显示强大的抗菌活性,不仅对甲氧敏感的金黄色葡萄球菌,链球菌pyrogenes和肺炎链球菌,但也克阳性菌为常规β-内酰胺类药物已证明无效,如青霉素耐药pneumococci ,口腔葡萄球菌和肠球菌,也显示出广泛的抗菌谱,包括革兰阴性菌如流感嗜血杆菌和厌氧菌如属杆菌,这活动是由于其新颖的骨架青霉烯环((化学ryoho没有ryoiki领域的化疗),第13卷,第10号,第74-80 , 1997年)。
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azoturia:氮尿
azotorrhea 氮溢 | azoturia 氮尿 | azurophil 嗜苯胺蓝的
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Heterotroph:异养菌
自养菌(autotroph)异养菌(heterotroph) 腐生菌 寄生菌 大部分病原菌 细菌的营养物质水碳源氮源无机盐生长因子growth factor:细菌生长必需,而自身不能合成的化合物 如维生素、某些氨基酸、嘌呤、嘧啶等 流感嗜血杆...
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nival:雪的
nitrophyte 嗜氮植被 | nival 雪的 | nival belt 恒雪帶
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nitrophilous:嗜氮的
nitrogeterdetector氮量计检测器 | nitrophilous嗜氮的 | nitroprotein硝基蛋的
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nitrophyte:嗜氮植被
nitrophilous vegetation 嗜氮性植被 | nitrophyte 嗜氮植被 | nival 雪的