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Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.
方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平,并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素,全自动血液凝固仪检测凝血酶原活动度,同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。
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Methods The concentrations of serum IMA and sICAM1 in 120 CHD patients were determined by ELISA and compared with 40 controls.
采用酶联免疫吸附测定法检测120例CHD病人血清IMA和sICAM1浓度的变化,并与40例对照者进行比较。
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There has been significant speculation that Navy dolphins are trained in attack missions, such as direct attacks against swimmers in the water or attaching limpet mines to enemy ships in harbor.
曾有大量猜测称,海军的这些海豚是受训来完成攻击任务的,如对水下游泳者的直接攻击或是将吸附式水雷贴附到港口中的敌军船只上。
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61Healthy persons were selected as healthy group. The serum concentration of TGF-β1 was measured by ELISA method; nitre oxide was measured by nitrit reductase method, plasma Ang II was measured by radioimmunoassay.
对高血压病人和61例健康对照者应用酶联免疫吸附法测定血清TGF-β_1浓度,硝酸还原酶法测定NO的浓度,放射免疫分析法测定Ang Ⅱ水平。
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ACA was detected in the sera of 176 patients and 98 members in control group by enzyme linked immunosorbent assay.
用酶联免疫吸附试验对176例急性脑血管病患者和98例健康对照者进行ACA检测。
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Methods The serum SIL-2R level in syphilitic patients of different stages and after therapy were detected using the ELISA technique.
采用酶联免疫吸附法测定 70例梅毒患者及其治疗后SIL - 2R水平,并与 2 0名健康体检者进行比较。
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Methods Monoclonal antibody turbidimetry and ELISA methods were used to detect the plasma Lp and CEA in 56 patients with large intestinal cancer and 87 healthy persons.
采用单克隆抗体浊度法和酶联免疫吸附试验法测定了 5 6例大肠癌患者和 87例健康者的血浆脂蛋白和 CEA的含量。
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Methods: With enzyme linked immunosorbent assay and micronize strengthening immunofixation, The serum OX-LDL and HS-CRP level of 215 patients with ACI was measured on the second day after hospitalization. In addition, 30 healthy volunteers were taken as the control group.
采用酶联免疫吸附试验、微粒化增强免疫固定试验测定215例ACI患者入院第2天血清中OX-LDL、HS-CRP含量水平,并与30例健康者作对照。
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Methods: 63 patients with ACVD and 26 age matched healthy volunteers were enroued. Plasmatic levels of TF and TFPI were examined with ELISA technique.
选择脑血管病急性期患者63例和健康体检者26例;采用双夹心酶联免疫吸附抗原测定法测定患者血浆中TF和TFPI的含量,与正常对照组及组间进行比较。
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Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V-Ⅲ by ELISA. Four patients ( 22.2%) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS.
对浙江省1982~1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者,用酶联免疫吸附法检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体,发现4例阳性,并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。
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paper chromatography:纸层析
利用各组分在凝胶上受阻滞的程度不同而进行分离.(5)亲和层析(Affinity Chromatography)根据生物特异性吸附进行分离,固定相只能和一种待分离组分有高度特异性的亲和能力者结合,而与无结合能力的其他组分分离.(1)纸层析(Paper Chromatography)以滤纸作为液体的载体,
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GIT:生长抑制试验
长出的菌落没有明显周边. 多次传代后生长加快,菌落成典型荷包蛋样. 分离的支原体可经形态、血细胞吸附、生化反应以及特异性抗血清作生长抑制试验(GIT)进行鉴定. 培养阳性率差,血清学诊断阳性者培养仅64%阳性.
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PCL:浆细胞白血病
方法 应用酶联免疫吸附试验定量测定38例浆细胞增生性疾病患者[浆细胞白血病(PCL)患者4例,多发性骨髓瘤(MM)患者24例,Waldenstrom巨球蛋白血症(WM)患者4例,6例意义未明单克隆免疫球蛋白血症(MGUS)患者]和健康体检者20例血清NDPK-A含量.