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Otocyst cells ; stem cells ; cell proliferation ; differentiation ; fetal rats
听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠
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Keywords otocyst cells; stem cells; cell proliferation; differentiation; fetal rats
听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠
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In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.
胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。
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CONCLUSION摘要: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.
结论摘要：胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。
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Month culture. CONCLUSION: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.
胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。
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The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.
本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。
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METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.
取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。
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METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.
方法摘要：取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。
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In 4 cases, the cavities were full of perilymph. We could find that the cavity was irregularly round-shaped, about 6.5 mm in diameter, with thin mucosa adhere to the cavity wall. In the center area, around the internal acoustic meatus fundus, there was a horseshoe- shaped cystic structure about 3 mm3, which was a combined structure of the saccule, utricle and scala media. In 1 case, there was little perilymph in the cavity, with about 1.5 mm thick soft tissue and granulation adhere to the wall. The saccule, utricle and scala media were not recognizable.
结果 对5例患者进行共同腔内耳蜗内镜检查，见4例腔内充满液体，吸出后腔壁有扉薄黏膜附着，腔呈不规则圆形，直径平均约为6.5 mm，中央区贴近内听道底部位见马蹄形或肾形囊腔，约 3 mm3大小，为内耳球囊、椭圆囊和中阶畸形融合的结构；1例腔内淋巴液较少，共同腔壁附着软组织和肉芽，约1.5 mm厚，无法将腔壁上的软组织与中阶、球囊、椭圆囊的结构区分。
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Results Five cases with common cavity underwent micro-endoscopy during cochlear implantation. In 4 cases, the cavities were full of perilymph. We could find that the cavity was irregularly round-shaped, about 6.5 mm in diameter, with thin mucosa adhere to the cavity wall. In the center area, around the internal acoustic meatus fundus, there was a horseshoe- shaped cystic structure about 3 mm3, which was a combined structure of the saccule, utricle and scala media.
结果 对5例患者进行共同腔内耳蜗内镜检查，见4例腔内充满液体，吸出后腔壁有扉薄黏膜附着，腔呈不规则圆形，直径平均约为6.5 mm，中央区贴近内听道底部位见马蹄形或肾形囊腔，约 3 mm3大小，为内耳球囊、椭圆囊和中阶畸形融合的结构；1例腔内淋巴液较少，共同腔壁附着软组织和肉芽，约1.5 mm厚，无法将腔壁上的软组织与中阶、球囊、椭圆囊的结构区分。
- 更多网络解释与听囊相关的网络解释 [注：此内容来源于网络，仅供参考]
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auditory tube：听管
\\"听骨或听石\\",\\"auditory ossicle\\" | \\"听管\\",\\"auditory tube\\" | \\"听囊\\",\\"auditory vesicle\\"
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auditory capsule：听泡, 听囊
parakite (可作降落伞用的)降落风筝 | monostyle [建]周围有一圈单列柱子的殿堂 | auditory capsule 听泡, 听囊
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auditory capsule：听囊
auditory acuity 听敏度 | auditory capsule 听囊 | auditory cell 听细胞
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otocyst：听泡
otic vesicle 听囊 | otocyst 听泡 | otolith 平衡石
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otocyst：听泡,听囊
\\"耳道闭合\\",\\"otocleisis\\" | \\"听泡,听囊\\",\\"otocyst\\" | \\"耳痛\\",\\"otodynia\\"
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utriculus：椭圆囊
2.听平衡觉器官(auditory organ)鱼类的听平衡觉器官是一对内耳,因其结构复杂而 称膜迷路(membrane labryinth),包藏于脑颅听囊内的外淋巴液中,膜迷路里充满着内淋 巴液.每侧的内耳都包括上,下二部分:上部是椭圆囊(utriculus)及与其连通的 3 个半 规管,
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collagenic fiber：胶原纤维
即管平衡听泡形成后其四周的间质组织 (mesenchymal tissue) 即变成听囊迷路,其与膜性迷路之间形成外淋巴间隙(perilymphatic space) 并蜗部分的外淋巴间隙分成二个部分,即鼓阶(scala tympani)及前庭成,其中间的胶原纤维(collagenic fiber)则为第一第二鳃弓的
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otic vesicle：听囊
otic capsule 听囊 | otic vesicle 听囊 | otocyst 听泡
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otic capsule：听囊
ostrone 雌酮 | otic capsule 听囊 | otic vesicle 听囊
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otic ve：听囊
otic capsule 听囊 | otic ve 听囊 | otter 水獭