台盼蓝

词组短语

trypan blue

与台盼蓝相关的网络例句 [注：此内容来源于网络，仅供参考]

Abstract］ Objective To explore the effect of radiotherapy on the auxesis of Hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.Methods After radiotherapy with different doses,the Hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.

目的 探讨放射对宫颈癌Hela细胞系再增殖的影响，宫颈癌是否有必要实行非常规分割放疗提供定的理论基础方法在不同剂量的放射作用下，以台盼蓝染色细胞计法分析Hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

Abstract］ objective to explore the effect of radiotherapy on the auxesis of hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.methods after radiotherapy with different doses,the hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.results （1）tpot shortening was observed in hela cell after radiotherapy.

目的 探讨放射对宫颈癌hela细胞系再增殖的影响，为宫颈癌是否有必要实行非常规分割放疗提供一定的理论基础。方法在不同剂量的放射作用下，以台盼蓝染色细胞计数法分析hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

Methods the rabbit arterial smooth muscle cells were cultured ex vivo, cells of 4-7 generation were divided into groups interfered with ang-（1-7）, positive control groupsinterfered with ang-ii(10-6mol/l) and blank control group .cell counting with trypanblau dying and mtt were used to evaluate the growth of cells in different groups and results were compared .

体外培养兔肺动脉平滑肌细胞，鉴定后传代培养，取4～7代细胞，应用不同浓度ang-（1-7）干预，并与血管紧张素-ii(ang-ii，浓度10-6mol/l)、空白组对照。通过台盼蓝染色法细胞计数、四甲基偶氮唑蓝比色法，确定肺动脉平滑肌细胞的生长趋势变化。

METHODS: MTT calorimetric method, trypin blue exclusion,colony formation and H22-bearing mouse model.

噻唑蓝法、台盼蓝拒染法、集落形成以及H22荷瘤小鼠模型。

Cell of Ela of 鳫 of fleeing Yu Piao, the stage longs for blue refus to catch a law to detect wither of cell of revulsive Hela of EVn-50 of observation of microscope of fluorescence of coloring of cellular vigor;AO/EB dies electrophoresis of gel of morphology change;DNA is corroborant wither of cell of EVn-50 revulsive Hela dies action;PI coloring sheds type cell appearance to detect wither of cell of EVn-50 revulsive Hela dies rate.

体外培养Hela细胞，台盼蓝拒染法检测细胞活力；AO/EB染色荧光显微镜观察EVn-50诱导Hela细胞凋亡形态学改变；DNA凝胶电泳确证EVn-50诱导Hela细胞凋亡功能；PI染色流式细胞仪检测EVn-50诱导Hela细胞凋亡率。

Stained with 0.4% trypanblau for 4 min, then counted the stained cells and unstained cells.

用0.4%台盼蓝直接染色4 min，显微镜观察，计数被染色细胞和拒染细胞。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

Trypan blue dye exclusion method and MTT assay were used to determine the uncytoxic dose ranges of PEA.

采用台盼蓝染色排除法及四甲基偶氮唑蓝法确定PFA对淋巴细胞的非毒性剂量范围。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

The tissue maintained the secretion activity under 72 h in roller tube culture, which was indicated by the MTT3-(4,5-dimethylthiazol-z-y1-2, 5-diphenyltetrazolium bro-mide value and oligonucleosomal fraction of cellular DNA.

通过台盼蓝染色观察、培养组织观察、测定 MTT 二甲基噻唑二苯基四唑嗅蓝活性和基因组 DNA 降解成为 1 000 bp 以下核苷酸片段量来比较两种方法的优缺点。

与台盼蓝相关的网络解释 [注：此内容来源于网络，仅供参考]

evans blue：埃文斯蓝

[摘要]HarpinXoo是水稻黄单胞细菌(Xanthomonas oryzae pv.oryzae)产生的蛋白类激发子,用harpinXoo注射烟草最早在处理后20h出现肉眼可见的细胞死亡(macro-HR),分别经埃文斯蓝(Evans blue)和台盼兰(Trypan blue)染色观察,

electron microscopy：电(子显微)镜术

2.不同浓度台盼蓝染色剂对晶状体前囊膜染色能力和效果的评价 3.动物实验 4.兔眼眼压测定 5.兔眼角膜内皮细胞计数及角膜厚度的检查 6.兔眼角膜、虹膜及前房角组织病理标本制作与观察 7.电子显微镜术(electron microscopy)观察角膜内皮细胞超

Triton X-100：曲拉通 X-100

TRIS-HCl 三羟甲基氨基甲烷盐酸盐 | Triton X-100 曲拉通 X-100 | Trypan Blue 台盼蓝

trying plane：大刨; 平刨; 细刨

trying contract 结卖合同 | trying plane 大刨,平刨,细刨 | trypan blue 锥虫蓝,台盼蓝

trypan blue：台盼蓝

(3) 材料:培养细胞.方 台盼蓝(Trypan Blue)法.法 (1) 试剂:用生理盐水配成 0.4%台盼蓝染液. (2) 染色制片:取 0.5ml 细胞悬液放入干净试管中,加入约 0.1ml(1~2滴) 染液, 混合, 2min 后立即制成临时装片,镜检. (3) 结果:死细胞染成蓝色,

trypan blue：台盼蓝,锥虫蓝

truffles 块菌[一类真菌] | trypan blue 台盼蓝,锥虫蓝 | trypanosome 锥虫

trypan red：台盼红

trypan blue 锥虫蓝,台盼蓝 | trypan red 台盼红 | trypan-blue injection method 台盼蓝流射法

trypanocidal：杀锥虫的

trypanblue 锥虫蓝 台盼蓝 | trypanocidal 杀锥虫的 | trypanocide 杀锥虫的

truffles：块菌[一类真菌]

troponin 肌钙蛋白 | truffles 块菌[一类真菌] | trypan blue 台盼蓝,锥虫蓝

trypan red：台盼红

trypan blue ==> 锥虫蓝,台盼蓝 | trypan red ==> 台盼红 | trypan-blue injection method ==> 台盼蓝流射法