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2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.
根据dystrophin基因5个多态位点(44、45、49和50号内含子以及5'端启动子区的序列)设计引物,在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾:CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身,经高分辨率的变性聚丙烯酰胺凝胶电泳后,利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置,进行扫描分析,确定产物片段的大小。
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The method is :there are two perpendicular axes whose distance are controlled ,one is the axis of work table, the other is fixed with a microscope for aiming.
该测量方法是:两个相互垂直且间距可调的数控回转轴,一轴与工件的回转中心重合,另一轴上固定可移动的瞄准显微镜,当显微镜瞄准被测孔时,两轴的转角由各自的圆光栅测出,两轴之间的角度位置确定曲面壳体零件上小孔的孔位置,同时采用CCD摄像头,利用图象处理技术实现小孔的尺寸测量。
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On the safe side, we should use active soil pressure and passive soil pressure to calculate the support strength before T increased. That is RA=EA-EP Among them: RA ………… holding strength EA ………… composite force of active soil pressure EP ………… composite force of passive soil pressure Immediately, flexural torque distribution of enclosure wall can be counted. Mmax is the location of shear force zero. Equivalence beam method abridges. No matter which kind of supporting structure form, the key problem lies in the establishment depth of catch point, severity of support and enclosure wall, penetration depth and pit bottom quality of soil property.
单道锚杆支护结构计算分别采用静力平衡法及等值梁法,根据试算法插入深度t值需先进行假定墙的插入深度确定后来计算支撑力,为了安全起见,可按插入深度增大前的主动与被动土压力合力计算支撑力即 RA=EA-EP 式中:RA …………支撑力 EA …………主动土压力合力 EP …………被动土压力合力随之可求围护墙的弯矩分布,Mmax为剪力为零点的位置,等值梁法的计算略述。
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IMRT is a new technology that is different from CRT. It uses multiple radiation beams that are broken up into many smaller beamlets. This allows radiation oncologists to precisely target the radiation to the tumor and spare the adjacent normal structures from high doses of radiation.
调强适形放疗是一种新型技术,它使用已经被分成众多细小光束的放射线,这可使放疗医生精确地确定肿瘤位置而临近正常组织不受到照射。
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relocatable:可重定位的
主要 存储器访问却可以在存储器的任何位置中且与一个不确定的起始位置相关.这样的代码被称为 是可重定位的(relocatable) ,而装入程序可处理所有的与指定的基地址或起始地址有关的可重 定位的地址.装入程序使得可执行代码更加灵活,
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placeable:可被确定位置的
placeability 可灌筑性 | placeable 可被确定位置的 | placebo 为死者所诵的晚祷歌