- 更多网络例句与可表达的相关的网络例句 [注:此内容来源于网络,仅供参考]
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The eukaryotic expression vector PBI121-CRP was constructed, then transformed into peanut (Arachis hypogaea L.) Heiba920 by agrobacterium-mediated transformation for expression. After the molecular detecting, the transformed plants were obtained.
构建真核表达载体PBI121-CRP,利用根癌农杆菌介导法导入黑花生,使其在花生中表达,经分子生物学检测后,获得可表达CRP的转基因花生植株。
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Modifier: is used to specify optionally expressible properties of heads.
详细说明了中心语的具有选择性的可表达的特征。
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Expressing the scepticism involves thinking oneself to have sensations; and the argument shows that if this thought is expressible, then it is also false.
表达怀疑论包含了思者自己拥有感觉;论证指明如果这个思是可表达的,那么它是错的。
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The results showed that human transferrin could be expressed in these cell lines and mouse or goat mammary gland following transfection, and its expression level could be improved dramatically when it was driven by rabbit enhancer and promotor as compared with goat beta lactoglobulin promotor. Transgenic mice were generated by either microinjection or lentivirus infection.
结果显示所有表达载体均可指导人转铁蛋白在细胞以及小鼠和山羊乳腺中表达,兔转铁蛋白增强子与启动子组合可显著性增加人转铁蛋白的表达水平,且其指导表达的效率明显高于山羊β乳球蛋白启动子。
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With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.
随着诱导时间的延长,表达的融合蛋白产量逐渐增加,表达蛋白总量最高可达到菌体总蛋白的39.14%;在15℃、25℃和37℃三个不同的诱导温度下,均能诱导产生融合蛋白,而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加;在菌体的各个部位中,融合蛋白主要在细胞质中以可溶的形式进行表达,有少量在外周质中表达或以包含体形式在细胞质中表达。
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The ability to modulate the expression of multiple genes by tetracycline and its derivatives is likely to regulate the timing and level of gene expression, quantitatively induce the expression of toxic protein, increase therapeutic efficacy and safety, and provide a safe and regulatable way for gene therapy.
CHO-Tet-On细胞株的建立,可利用四环素及其衍生物调节多种外源基因的表达,有效调控基因表达的时间和水平,定量诱导毒性蛋白的表达,增加治疗的疗效和安全性,有望为基因治疗提供一条可控的安全途径。
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The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.
研究结论:(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化;(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠,使外源AT2R基因的表达处于有效的主动控制下;(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生;(4)由MSC介导的AT2R可调控表达受到Dox的良好调控,可有效抑制球囊损伤后大鼠颈动脉新生内膜增生;(5)AT2R影响血管损伤后修复时ECM成分的表达,并促进细胞凋亡的发生,可能是AT2R介导抑制新生内膜形成的机制。
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①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.
研究方法:(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC,细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构;(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定;(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC,并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况,根据各个细胞克隆受Dox调控表达的程度,选择低背景、高诱导表达AT2R的细胞系,作为双重稳定MSC细胞系;蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况;(4)建立大鼠颈动脉球囊损伤动物模型,将双重稳定MSC在术中导入血管,分别于14 d、28 d进行病理切片,检测可调控AT2R对新生内膜增生的影响;采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变,TUNEL法检测血管组织中细胞凋亡的变化情况。
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(1)raising of PCNA protein expression after pre-operative radiotherapy of esophageal carcinoma may signify the repopulation of tumor cells.(2) The prognosis differed from different PCNA expression before or after radiation, it may be beneficial to select the best treatment plan and as the evaluating criteria.
(1)食管癌放疗后PCNA蛋白表达升高,可能存在加速再增殖;(2)疗前、疗后PCNA蛋白表达的预测性不同,可指导临床选择最有效治疗方案,可作为评价预后指标。
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Once guests step into the yard, delicious odor, glorious leaves and pink or snowy white flowers greet them softly 这句是够简练的了,可表达不出原文的意思. It warms the cockle of their heart as if being home.
客人一走进大门,扑鼻的是一阵幽香,入目的是绿蜡似的叶子和红霞或白雪似的花朵,立刻就感觉到仿佛走进自己的家门口,大有宾至如归之感了。
- 更多网络解释与可表达的相关的网络解释 [注:此内容来源于网络,仅供参考]
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comprehensible:可理解的
此外,确定评分标准时还应吸收交际法测试的两个特征:对语法要求宽松;可理解的(comprehensible). 即在确定评分标准时以语法正确错误作为评分的唯一依据,应以达意和理解放在首位,以利于学生敢于表达,积极交流. 所谓"可理解的"原则,
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conceivable:可想像的
conceit 自负;意念 | conceivable 可想像的 | conceive 构想;表达
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effaceable:可抹掉
effable 可表达的 | effaceable 可抹掉 | effaceeffacementerasion 抹杀
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expresser:表达者
expressage 快递 | expresser 表达者 | expressible 可表现的
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expressible:可表达性:可以口语化表达
..Consistent.一致性:规则不能有逻辑上冲突 | ..Expressible.可表达性:可以口语化表达 | ..Business-Oriented:企业可以理解的,重要的参与感
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get involved in doing sth:参与到做某事中, 骗人的玩意儿 (很好表达出一些专家的不屑)
star-gazing parties观星派对,美好D未来 | get involved in doing sth 参与到做某事中, 骗人的玩意儿 (很好表达出一些专家的不屑) | sustainable future 可持续的,
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get involved in doing sth:参与到做某事中 玩意儿 (很好表达出一些专家的不屑)
sustainable future 可持续的,美好D未来 | get involved in doing sth 参与到做某事中 玩意儿 (很好表达出一些专家的不屑) | sustainable future 可持续的,美好D未来
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revokable:可废除的
revoice 重新表达 | revokable 可废除的 | revoke 撤销
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effable:可表达的
efedrina 麻黄碱 | effable 可表达的 | effaceable 可抹掉
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revoice:重新表达
revocatory 撤销的 | revoice 重新表达 | revokable 可废除的