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Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.
首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I,并成功地在原核细胞E.coli进行了重组表达,筛选出稳定表达的工程菌株DH5α,摸索了稳定表达的条件,摸索了包涵体的洗涤、变性和复性条件,摸索出了复性蛋白Src I的纯化工艺,纯化过程简单,经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化,就可获得了高纯度的重组蛋白样品(纯度达99%以上),该纯化方法适合重组蛋白的大量纯化,为Src I的性质、结构和功能研究奠定了良好的基础。
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Preparation of the cathode includes:shaping under the press of 40Mpa, sintering at 550℃for 1 hour and at 900℃for 8 hours and threading with molybdenum bar; Considering the literatures we choose CaCl2 as salt for preparation titanium. Pretreatment of salt is for 1 hour at 100℃and for 2 hours at 300℃. Partial pressure of oxygen which need lower than 5.11×10-7Pa to reduct titanium oxides and hygroscopic property of salt need a sealed equipment to electrolyse. And finally successfully designed a satisfied one and the results show that the equipment can be satisfied the requirment of the experiment. Flow of the inert gas is 1.5L/min, the voltage is 2.8 V, temperature is 850℃and time is 2 hours during pre-electrolysis. Flow of the inert gas is 0.2L/min, the voltage is 3.1 V, temperature is 900℃and time changes with the mass of TiO2 during electrolysis, namely the greater need the longer time; To eliminate influence of salt and other impurities, the products need to wash with distilled water and dilute chlorhydric acid , then wash with dilute hydrochloric acid under supersonic wave assistant. Finally, electrometical properties of the electrolysis of TiO2 is researched by cyclic voltammetry and chronoamperometry, and results show that there are two main reodox steps, namely from TiO2 to TiO and from TiO to Ti.
阴极制备主要包括40MPa压力下模压成型、两段式烧结(1小时内升至550℃保温1小时,再1小时升温至900℃保温8小时)及烧结后TiO2块打孔用钼棒串接三个主要环节;实验中选用CaCl2作为电解熔盐,并对其进行预处理(100℃,保温1小时; 300℃,保温2小时);经热力学计算,还原钛氧化物的氧分压至少要低于5.11×10-7Pa,结合电解过程中所用熔盐CaCl2有极强的吸水性的特点,电解装置应有较高的密封性,自行设计了一套密封性可靠的电解装置,便于实验过程中熔盐预处理和氧分压的控制;通过干燥处理预电解过程中Ar流量大约为1.5L/min、电压为2.8 V、温度为850℃、时间为2小时,电解过程中Ar流量大约为0.2L/min、电压为3.1V、温度为900℃,实验结果表明电解时间与TiO2质量密切相关,质量越大需要电解的时间越长;通过自来水冲洗—稀盐酸浸泡、洗涤—在超声波辅助作用下稀盐酸洗涤,可减少熔盐及其它杂质对电解产物检测结果的影响;最后,通过循环伏安法、计时电流法对电解机理的研究,确定电解还原TiO2制备金属钛主要经历了TiO2-TiO-Ti的过程。
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Analysis on main points for determination of chlorine dioxide by iodometric method ;2. The experiment results show: To measure sulfide in wastewater by iodometric method may have errors because the interferent can not be removed.
用碘量法测定废水中的硫化物,可能因无法消除干扰而使测定结果存在较大误差;对于同一废水样品,当碘量法和亚甲基蓝分光光度法的测定结果存在显著性差异时,可用Pb2半定量法进行判定;对于某些化纤企业的废水,用氢氧化钾-乙醇溶液洗涤沉淀,可消除碘量法测定的干扰。
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Sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine is often applied to producing bifunctional-soluble reactive dyes and is an important dye intermediate. It plays an important role in the synthesis of KN style reactive dye M style double reactive dye and modified dissimilar double reactive dye. Used 2-sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine as raw material can improve the uniformly dying performance and scourablility of dyestuff molecules and can improve the firm degree of colour staining.
2-磺酸-4-苯胺是制备水溶性双活性染料的重要中间体,在KN型活性染料、M型双活性染料以及改性异种双活性染料的合成中起着重要的作用,用2-磺酸-4-苯胺作原料合成活性染料可增加染料分子的匀染性和易洗涤性,提高染料的粘色牢度。
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Using hydrotreated gas oil as solvent, under the conditions of a reaction temperature of 400 C, a hydrogen partial pressure of 10 MPa and a reaction time of 1 h, a portion of coke deposits could be removed yet without the function of hydrogenating coke.
采用溶剂萃取法除去工业失活催化剂上沉积的焦炭和金属的研究结果表明,用四氢呋喃、吡啶和喹啉这3种含杂原子的有机强溶剂常温萃取催化剂,其去除焦炭能力很弱,而且会因这些溶剂的强吸附作用污染催化剂;盐酸洗涤能有效去除催化剂表面的钙、铁沉积层,但不能去除焦炭;低芳香性的加氢瓦斯油在400 C、H2压力为10MPa、反应1h的条件下可部分去除催化剂上的焦炭,但不具备强的氢化焦炭作用;1,2,3,4-四氢化萘因具有供给原子氢的能力,可以有效地氢化和去除催化剂上的焦炭。
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Excellent properties in lime soap dispersing, washing, penetrating, foaming, emulsification, leveling and softening
具有优良的钙皂分散、洗涤、渗透、起泡性、乳化、匀染、柔软等性能。◆可改善提高织物的水洗牢度。
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Fabrics are manipulated: tweeds are given a thicker, more open weave, as if knitted by hand. Lived in cottons are treated with heavy washes for a worn look, while soft jersey features in long fitted wool coats, wonderfully comfortable to wear. Even leather is imbued with a new "stretch" quality for maximum lightness and practicality.
布料的发挥亦游刃有余:崭新的厚身寛松花呢仿如全人手编织;棉质则预先经过多重洗涤处理,营造出二手的效果;至于柔软的针织以长身的修身羊毛衣形式登场,舒适感无可比拟;即使是一贯线条硬朗的皮革,今季亦注入了温柔的「弹性」元素,带来高度轻身效果,同时增加了可穿性。
- 更多网络解释与可洗涤性相关的网络解释 [注:此内容来源于网络,仅供参考]
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cleavability:可劈性
clearing device 洗涤器 | cleavability 可劈性 | cleavage 劈理
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synthetic leather:合成革
摘要: 聚氨酯合成革(Synthetic Leather)基本知识 聚氨酯合成革属于聚氨酯弹性体的一类,具有光泽柔和、自然,手感柔软,真皮感强的外观,具有与基材粘接性能优异、抗磨损、耐挠曲、抗老化等优异的机械性能,同时还具备耐寒性好、透气、可洗涤、加工方便、价格优廉等优点,
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washability:可洗涤性;洗净程度;洗涤能力
wash-wear 耐洗的 | washability 可洗涤性;洗净程度;洗涤能力 | washbox 洗涤箱