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Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.
以同一母体来源的白念珠菌CA-1~A-17(共16株)作为研究对象,采用RPMI1640和DMEM培养基,37℃培养24h诱导其假菌丝形成,记录不同观察时间时的菌丝相和酵母相细胞数,计算菌丝相白念珠菌形成率;以RPMI1640培养基,37℃连续传代培养7天(转种12次)后,分离菌丝相白念珠菌并提取其基因组DNA;以EcoRⅠ和BglⅡ消化菌丝相基因组DNA,消化产物进行琼脂糖凝胶电泳并拍照;用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA,采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。
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anacrusis:行首额外音节
anacrobictank 加水分解池 | anacrusis 行首额外音节 | anaculture 变性培养物
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anaculture:变性培养物
anacrusis 行首额外音节 | anaculture 变性培养物 | anademchapletcoronal 花冠