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Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.
检测了132株，没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA，作PCR检测。D1代获得5株阳性转化植株，分别是90035的4株，85—593的1株，转化率为3.8％。6、将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。
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BA and GA3 in proper concentrations were the effective hormones to induce the germination of germ tube and the germinating rate was 3.3%. When treated with 6-BA + GA3 + IBA, the germinating rate doubled compared with single hormone treatments. However, the germ tube didn't form haustorium afterwards.
结果如下： 1、适当浓度的6-BA和GA3能够诱导肉苁蓉种子萌发形成芽管，芽管萌发率仅为3.3%，6-BA + GA3 + IBA组合使种子芽管萌发率提高2倍，激素只能诱导肉苁蓉种子萌发芽管，不能进一步诱导肉苁蓉种子形成吸器。
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About 12 months earlier, the pollen grain germinated, producing a pollen tube that slowly digested its way through the tissues of the nucellus toward the developing megagametophyte.
大约 12 年之前，花粉谷粒发芽，产生慢慢地经过向发展中的 megagametophyte 的 nucellus 的薄的纱织品消化了它的方法的花粉管。
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Ten days post meiosis, megaspore gave rise to the one nucleate embryo sac.
花粉粒在授粉后三天发芽长出花粉管。
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Shortly after the pollen grain comes in contact with the nucellus, it germinates to form a pollen tube.
在不久之后，花粉谷粒用 nucellus 进来连络，它发芽形成花粉管。
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appressor：附著器
"伏毛","appressed hair ; adpressed hair" | "附著器","appressor" | "附著器;菌丝或发芽管的膨大末端","appressoriurn"
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stomatal conductance：气孔导度
蜡有时积聚在针叶树气孔的口孔,以减低气孔导度(stomatal conductance). 角质可以阻碍发芽真菌孢子的入侵. 但是真菌也会产生角质分解酵素(cutinase)分解角质形成通道,供真菌孢子发芽管穿透之用. 同化作用(assimilation)意指转变碳水化合物及氨基酸成为新的组织.